Contributions to the taxonomy of the genus Flavobacterium.
Explore the source record for details and available documents.
Antigens, Bacterial↗
Biomedical subjects
B Bruun
Publications and source records attributed to B Bruun.
Kinetics of cefuroxime in the groin wound after vascular prosthetic implantation.
The kinetics of cefuroxime in serum and wound fluid were investigated in the period after vascular prosthetic implantation. Cefuroxime was administered as intravenous bolus injections in doses of 0.75 gm (five patients) or 1.5 gm (five patients). The concentration of cefuroxime in wound fluid increased after the injection and reached a maximal level corresponding to serum concentration levels with 1.5 hours. The subsequent elimination of cefuroxime from the wound fluid closely paralleled the elimination from the serum. The wound fluid concentrations were found to be greater than the minimum inhibitory concentration for Staphylococcus aureus and Escherichia coli in 8 and 5 hours, respectively, after injection of 0.75 gm of cefuroxime, and in 11 and 7 hours, respectively, after injection of 1.5 gm of cefuroxime.
Aged↗
[Health visitors' dilemma: she is best when she is "invisible". Interview by Lars Peter Bergqvist].
Explore the source record for details and available documents.
Community Health Nursing↗
Genetic heterogeneity of Flavobacterium meningosepticum demonstrated by DNA-DNA hybridization.
DNA-DNA reassociation studies on 52 strains of Flavobacterium meningosepticum showed two main hybridization groups about 40-55% interrelated and comprising 4 and 48 strains respectively. The larger group could be further divided into four subgroups if differences in thermal stability of the reassociated duplexes were taken into consideration. Of a total of 20 strains isolated from cerebrospinal fluid, 18 were found in one subgroup of 28 strains, which also contained seven blood isolates. The results indicate that genetically defined subgroups within the species might differ with regard to pathogenic significance.
DNA, Bacterial↗
Phenotypic characterization of Flavobacterium meningosepticum strains identified by DNA-DNA hybridization.
Fifty-two strains found to belong to Flavobacterium meningosepticum on the basis of DNA-DNA hybridization analyses were characterized and found to be phenotypically homogeneous. All strains were oxidase, catalase, indole, gelatinase and beta-galactosidase positive, and produced acid from glucose, mannose, fructose, maltose and mannitol; nitrate was not reduced. F. meningosepticum could be differentiated from Flavobacterium group IIb by its ability to produce beta-galactosidase, and by the latter taxon's ability to produce a bright yellow pigment in contrast to the weak or non-existing pigmentation of F. meningosepticum. Phenotypic characteristics that could differentiate between the two main DNA relatedness groups of F. meningosepticum were not discovered, wherefore a subdivision of the present species into two species cannot be recommended. Strains from the DNA relatedness groups comprising 19 of 20 CSF isolates were found to be able to grow at 40 degrees C and to produce a weak yellow pigment; in contrast, strains from the three other DNA relatedness groups were unable to grow at 40 degrees C and produced no pigment.
DNA, Bacterial↗
Antimicrobial susceptibility of Flavobacterium meningosepticum strains identified by DNA-DNA hybridization.
Antimicrobial susceptibilities of 52 strains of Flavobacterium meningosepticum were determined by an agar plate dilution technique and by a tablet diffusion method. No useful susceptibility differences between the two main DNA relatedness groups within F. meningosepticum were found. All strains produced beta-lactamase and were resistant to most beta-lactam antibiotics. Ciprofloxacin, rifampicin and clindamycin appear to be the most promising for treating infections caused by F. meningosepticum.
Anti-Bacterial Agents↗
Crossed immunoelectrophoretic analysis of Flavobacterium meningosepticum and allied Flavobacterium taxa.
A total of 55 different antigens were demonstrated in pooled lysate of six Flavobacterium meningosepticum strains by crossed immunoelectrophoresis against homologous rabbit antibody. The six strains used represented the two DNA relatedness groups within this species. All 52 strains of F. meningosepticum investigated were found to cross-react with 53 to 55 of these F. meningosepticum reference antigens. Using this F. meningosepticum reference system, cross-reactions between the F. meningosepticum reference antigen and antigens from representatives of other Flavobacterium taxa were also investigated. A positive correlation was demonstrated between the number of cross-reacting antigens and the taxonomic relatedness of the investigated taxa as determined by other classification methods, including DNA-DNA hybridization. The crossed immunoelectrophoretic techniques were not useful in differentiating the two main DNA groups of F. meningosepticum.
Animals↗
Branhamella catarrhalis: significance in pulmonary infections and bacteriological features.
A three-month survey revealed 29 patients at our hospital with symptoms of acute pulmonary infection, from whom Branhamella catarrhalis was isolated from lower respiratory tract specimens, in 18 cases in pure culture. Approximately 2% of all respiratory tract specimens examined during the period yielded growth of B. catarrhalis. All except one patient suffered from chronic pulmonary disease, notably chronic bronchitis. A phenotypic comparison was made between 55 strains of B. catarrhalis, of which 50 were recent isolates from lower respiratory tract specimens, and 23 Neisseria strains representing Neisseria meningitidis, Neisseria gonorrhoeae, Neisseria cinerea, Neisseria flavescens, Neisseria mucosa, Neisseria pharyngis, and Neisseria lactamica. The morphology of B. catarrhalis colonies is very characteristic, and when the diagnosis is suspected, testing for the ability to hydrolyze tributyrin may confirm it within hours. Ability to produce deoxyribonuclease is another property which differentiates B. catarrhalis from the Neisseria species. Otherwise, the combination of nitrate reduction and failure to produce acid from glucose, maltose, and sucrose establishes the diagnosis.
Aged↗
Value of CSF lactate in the differential diagnosis between bacterial meningitis and other diseases with meningeal involvement.
Lactate concentrations in the cerebrospinal fluid of 104 patients were determined by the Monotest Lactate Kit. Lactate values were found higher in cases of bacterial meningitis than in patients not suffering from acute CNS disorders. Elevated lactate levels were also found in patients suffering from aseptic meningitis, septicemia, CNS trauma and cerebrovascular accidents, seizures and diabetes mellitus. The highest levels were found in cases of bacterial meningitis, but there was considerable overlapping between the groups. CSF lactate thus appears to have limited diagnostic value in the differential diagnosis between bacterial meningitis and other diseases with meningeal involvement.
Adolescent↗
Bacteremia caused by a beta-lactamase producing strain of Branhamella catarrhalis. A case report.
A case of Branhamella catarrhalis bacteremia without known infectious focus in a granulocytopenic patient is reported. There were no unusual clinical features of bacteremia, such as purpura, in this case which distinguished it from bacteremia due to other bacteria. The isolated strain was beta-lactamase producing. The patient recovered uneventfully on antibiotics; at the same time, however, the bone marrow was also normalized.
Aged↗
Actinomycotic infections of the central nervous system. Two case reports.
Two cases of CNS infections caused by Actinomyces israelii one a fatal case of brain abscess and the other a case of subdural empyema with complete recovery, are reported. One of the patients suffered from a congenital ventricular septal defect, and in both patients a pulmonary focus was found. When slow-growing bacteria such as Actinomyces may be suspected, it is important to cultivate anaerobically for at least five days.
Actinomyces↗
Phenotypic differentiation of Cardiobacterium hominis, Kingella indologenes and CDC group EF-4.
Eleven strains of Cardiobacterium hominis, two strains of Kingella indologenes and six strains of CDC group EF-4 were characterized. Since all three taxa are oxidase-positive, fastidious gram-negative rods with relatively few positive reactions, they may be easily confused in the microbiological laboratory. Common characteristics are acid production from glucose, aerobic growth in semi-solid agar and very slow anaerobic growth. Group EF-4 is distinguished from the other two species by its capacity to produce catalase and its lack of indole production. Cardiobacterium hominis can be distinguished from Kingella indologenes by its ability to ferment mannitol and sorbitol and by differences in enzyme production. The three taxa also differ considerably with regard to morphological and cultural characteristics.
Aerobiosis↗
Value of pleural lactate in the differential diagnosis between empyema and non-bacterial pleural effusions.
Lactate concentrations in the pleural fluid of 50 patients were determined by the Monotest Lactate Kit. Lactate values were found higher in bacterial pleural infections than in cases of hydrothorax. Elevated levels were also found in most cases of histologically confirmed cases of pleural neoplasms and in some cases of non-bacterial pleuritis exsudativa . The highest levels were found in cases of empyema, but there was considerable overlapping between the groups. Pleural lactate thus appears to have little diagnostic value in the differential diagnosis between empyema and non-bacterial pleural effusions.
Adult↗
Recognition of coagulase-negative Staphylococcus aureus strains by primary polymyxin susceptibility testing.
A prospective study evaluating the usefulness of polymyxin susceptibility testing in unveiling coagulase-negative S. aureus strains was undertaken. During a six months study period, 14 staphylococcal isolates from four patients were initially found to lack coagulase activity and to be polymyxin resistant; in comparison, approximately 1500 ordinary, coagulase-positive, polymyxin resistant S. aureus isolates were found during the same period. One isolate from each of the four patients together with a previously isolated coagulase-negative S. aureus strain from our own collection were further characterized. Two of the strains turned out to show delayed coagulase activity on retesting, while the other three strains failed to produce clotting in any coagulase assay. Judged by thermostable nuclease activity, phage typing and biochemical profiles these three coagulase-negative strains are bona fide S. aureus strains. The polymyxin test thus appears to be useful in disclosing S. aureus variants not identified as S. aureus by routinely performed coagulase assays.
Animals↗
Bacteremia caused by a beta-lactamase producing Haemophilus parainfluenzae strain of a new biotype. A case report.
A case of Haemophilus parainfluenzae bacteremia without known infectious focus is reported. Phenotypically, the isolated strain is a typical H. parainfluenzae except for its ability to produce indole and beta-lactamase. Beta-lactamase producing H. parainfluenzae organisms are encountered occasionally, but to the best of our knowledge this is the first reported blood culture isolate with this ability. We propose a new biotype (IV) of H. parainfluenzae to accommodate strains that are indole, urease and ornithine decarboxylase positive.
Aged↗
[Bacteremia in 1977-78. Bacterial diagnoses and antibiotic sensitivity].
Explore the source record for details and available documents.
Anti-Bacterial Agents↗
Studies on a collection of strains of the genus Flavobacterium. 2. Nutritional studies.
An attempt has been made to further characterize the Flavobacterium groups found in an earlier study by determining utilizable carbon-energy sources. As a preliminary, it was initially tried to define minimal growth factor requirements. It was found likely that most of the Flavobacterium groups have amino acid requirements, but a requirement for vitamins was also established for some strains. Testing Flavobacteria for carbon-energy sources by the replica plating method was found sufficiently technically difficult as to result in dubious readings at times. However, the overall results of the carbon-energy tests are in good agreement with the subdivision resulting from the previously performed biochemical tests, and the validity of the groups delimited in the earlier study is confirmed.
Amino Acids↗
Polymyxin susceptibility in staphylococci differentiating coagulase-positive and coagulase-negative strains.
47 staphylococcal reference strains representing 13 species were tested for polymyxin sensitivity using tablet and disc diffusion methods. Corresponding MIC and IC50 values were determined with a plate dilution assay. Coagulase-positive strains were found to be more resistant towards polymyxin, and could thereby be separated from coagulase-negative strains. Furthermore, 100 recently isolated staphylococci from clinical specimens were tested, and again the coagulase-positive strains could be identified by their smaller tablet inhibition zones. We conclude that the polymyxin test is an aid in the fast differentiation of staphylococci in laboratories performing primary sensitivity tests.
Coagulase↗