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Biomedical subjects

B Brinkmann

Publications and source records attributed to B Brinkmann.

At least 307 records · Page 17Linked to original sources
PubMed1979

[Typing ACP isoenzymes in blood stains: test limits and temperature dependancy (author's transl)].

Two series of test blood stains of known red-cell acid phosphatase types A, AB, AC, B, BC, and C are applied to two different types of stain carriers (glass and cotton) and stored at different temperature ranges: (a) 37 degrees C at high air humidity, (b) 22 degrees C, (c) 4 degrees C, and (d) - 20 degrees C. Electrophoresis is carried out either on cellulose-acetate-foils or on thin agarose layers. Visualisation of the isoenzyme sites is achieved using umbelliferyl phosphate as a substrate. The most important results are: Electrophoresis on "Cellogel"-foils turns out advantageous compared to agarose since (a) less stain material is required, (b) reproducibility is rather reliable, and (c) electrophoretic separation is clearer. Blood stains on glass are identified after longer periods of storage than stains on cotton. If stains are stored at room temperature the time limits of demonstration are in the range of 3 to 6 weeks. The size of the sample necessary for demonstrating varies widely. Approximately twenty to twenty-five per cent of a blood drop are needed. The method described is recommended as practicable and reliable.

Acid Phosphatase↗
PubMed1978

Heat injuries to the respiratory system.

A steam-tube of the main boiler exploded on a ship lying in the harbour of Hamburg. The steam temperature was 283 degrees C. Cutaneous and severe inhalational scalding occured in the 27 fatalities, the men dying after different intervals. This paper deals with the pathological findings in the respiratory passages and the lung, describing the topographical extent of direct thermal injury and the temporal course of tissue reactions. In cases of instantaneous death coagulation necrosis of the tracheal and bronchial wall was found to extend to alveolar ducts in central parts of the lung. The lung parenchyma showed marked congestion, alveolar edema and desquamation of alveolar epithelial cells. Death occured due to acute pulmonary dysfunction and shock. Lethal complications following the period of primary shock consisted of fulminant confluent bronchopneumonia, the hyaline membrane syndrome or the onset of desquamative interstitial pneumonia. These changes rendered it difficult to evaluate the effects of the heavy cutaneous scalding on the pathological course of inhalational injuries in those surviving for longer periods.

Adult↗
PubMed1978

[About the polymorphisms of esterase D and glyoxalase I and their forensic application (author's transl)].

Phenotypes of esterase D and glyoxalase I were determined electrophoretically in samples from Northern Germany. Gene frequencies: EsD1 = 0,889 (n = 1430), GLO1 = 0,415 (n = 865). The data of 295 (EsD) and 153 (GLO) mother/child combinations were in full accordance with the genetic model of two codominant alleles at an autosomal locus. Esterase D typing has proved successful in bloodstains up to three weeks old. The forensic scope of serological systems is discussed intensively. According to our own data and the results presented in the literature esterase D and glyoxalase I have proved to be useful additional markers for use in cases of disputed paternity.

Blood Group Antigens↗
PubMed1978

[Malignant hyperthermia during the 13th general anaesthesia (author's transl)].

We report a case of malignant hyperthermia in a man of 41 years during his 13th general anaesthesia. All previous anaesthetics were quite normal. Musculoskeletal abnormalities and increased CPK-levels are to be found in some members of the patient's family. The combined use of suxamethonium and halothane might have caused the development of malignant hyperthermia. As a concept of the aetiology of the syndrome the case history indicates that it may be stress-related.

Adult↗
PubMed1978

[Epidemiological aspects of malignant hyperthermia (author's transl)].

A statistical review is given on cases of malignant hyperthermia in Hamburg. The total number of general anesthetics from 1970--1976 was 960 000. 10 cases of malignant hyperthermia have been observed so that a crude estimate of the incidence would be about 1 : 100 000. Some epidemiological aspects of the patients in Hamburg are discussed.

Adolescent↗
PubMed1976

[Forensic usefulness of coronarometric studies (author's transl)].

Authors examine the forensic usefulness and scope of angiometric studies in cases of sudden death that are presumably caused by coronary heart disease (CHD). Hearts of 35 men aged between 40 and 59 years who died of CHD are studied angiographically, morphologically and microscopically. The control group consists of 34 men of the same range of age who died of unnatural causes. Following to a schematic model with special regard to hemodynamic aspects and the degree of the stenosis a stenosis score is made up for each heart and correlated on a diagram with the corresponding heart weight. It appears that the cases of both groups are different areas but are showing a certain degree of overlap. The variation within both groups is discussed. With regard to the CHD group it is presumed that the factors causing death are too variable as to be defined by the deviced methods exclusively. The causal role of factors other than atherosclerosis in the occurrence of CHD is discussed. Authors conclude that the methods examined could be useful in special forensic problems.

Adult↗
PubMed1976

[Post mortem temperature equilibration of the structures of the head. I. Thermometric techniques and principal investigations (author's transl)].

Special thin and flexible thermometric probes showing a diameter of 1 mm and a sharp end were used for post mortem (p.m.) thermometric studies in several tissues. Brain temperatures were measured by inserting a double probe through the superior orbital fissura thus allowing to record the central and the peripheral brain regions separately. Another probe was inserted into the galea and a fourth into the liver. Temperature changes were recorded simultaneously. Many variables of the human head were measured. Sixteen corpses were investigated. The results were as follows: 1. Of all temperature curves registered those of the central brain regions showed the smallest variance. 2. The p.m. temperature curve of the brain shows a sigmoid shape with a rather short "plateau" in the beginning. 3. In the early p.m. phase there is an increasing difference of temperatures between central and peripheral brain regions amounting to 2-4, 6 degrees C in the time period between 78th and 128th minute. 4. The insertion of the thin probes does not cause visible damages. Thus it should be considered for use in forensic practice. 5. Some artificial "head models" were constructed and temperature decrease recorded after warming. The curves showed the same type of sigmoid shape as those obtained from the corpses. 6. Of the possible variables measured that could influence the temperature decrease only the density of the hair seems to be of interest.

Brain↗
PubMed1976

[Fat embolism of the lungs as the cause of death. Etiology, pathogenesis and reasoning (author's transl)].

A comprehensive survey is given of the most important theories of etiology, pathogenesis and pathophysiology of pulmonary fat embolism. Autopsy and microscopic examinations were performed on 26 corpses with different causes of death. The total contents of fat of the lungs was analyzed by chloroform extraction. Death due to pulmonary fat embolism was diagnosed in three cases. In those with whole fat contents of about 20 g or more the histological degree was equivalent to occlusion of half of all vessels. The histological feature resembled that of shock; hemorrhage and microthrombosis were regular findings. The functional and morphological relationships with shock are discussed. It is concluded that, at least in the initial stage, fat embolism is due to mechanical injury. This mechanism is possibly altered or enhanced by biochemical changes in later phases. Pulmonary fat embolism is a disease which may cause death if mechanical, toxic and shock-inducing effects lead to insufficiency of the cardio-respiratory system. In forensic cases death due to fat embolism should be diagnosed only after exclusion of other lethal factors. Posttraumatic cases often show competition with shock due to hemorrhage. It is important to relate the degree of fat embolism to preexisting diseases.

Adult↗