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Biomedical subjects

B Anderson

Publications and source records attributed to B Anderson.

At least 397 records · Page 22Linked to original sources
PubMed1979

Development of DES-associated clear-cell carcinoma: the importance of regular screening.

Because the rate of malignancies in young women exposed in utero to diethylstilbesterol (DES) is low, appropriate population screening methods have not been established. A case is presented that is believed to represent the first reported instance of a DES-exposed daughter who developed clear cell adenocarcinoma of the vagina after initially negative examinations. The patient was followed with Papanicolaou smears, pelvic examinations, and colposcopy every 6 months for 2 years prior to the discovery of malignancy. Initially negative, Papanicolaou smears successfully predicted the presence of an early adenocarcinoma. Palpation aided by colposcopy allowed directed biopsy of the small asymptomatic lesion. This case underscores the necessity for frequent vaginal cytologic smears and pelvic examinations at intervals no greater than 6 months. Colposcopy is indicated to direct biopsies when an abnormal cytologic smears is reported or when abnormal bleeding or discharge occurs. Biopsy of any palpable lesion is mandatory.

Adenocarcinoma↗
PubMed1978

Bruising in cattle transported over long distances.

The relative susceptibility of cattle of different sexes to bruising was examined in five trials. In each trial there were three groups of cattle--bullocks, cows and mixed (bullocks and cows). The weight of bruised tissue trimmed from the carcases of the cows was significantly greater than that trimmed from the carcases of bullocks. The amount of bruising in cows, but not in bullocks, increased with increase in duration of journey. There was no evidence to suggest that differences in carcase weight or temperament, or "riding" of cows in oestrus were major contributing factors to the different levels of bruising.

Animals↗
PubMed1978

Improved susceptibility disk assay method employing an agar overlay technique.

Susceptibility disks impregnated with 35 different antibiotics were assayed by the current Code of Federal Regulations method and by a new method employing an agar overlay. The new method, a simplified analytical procedure, exhibited improvement in zone definition and an increase in dose response. Assays performed by the new method were accurate and precise. The agar overlay technique appears to be superior to the current Code of Federal Regulations method.

Culture Media↗
PubMed1978

Stereospecific antibodies to methadone. II. Synthesis of d- and 1-methadone antigens.

Diastereomeric methadols were prepared by reduction of the methadone enantiomers. Resulting methadols, after purification and characterization by mass spectrometry, were succinylated. The hemisuccinyl derivatives were conjugated with thyroglobulin via carbodiimide condensation. The conjugates of d- and of 1-methadol hemisuccinyl-thyroglobulin were employed as the antigens for immunization of white New Zealand rabbits in order to obtain specific anti d- and anti 1-methadone antisera.

Animals↗
PubMed1978

Hyperoxic myopia.

We have reported the development of 1.6 diopters of myopia in a group of patients exposed to reptitive oxygen breathing at two atmospheres absolute pressure. No significant change in corneal curvature accompanied this refractive change. During the three months following termination of the hyperoxic exposure, the myopia gradually disappeared. It is speculated that hyperoxygenation alters the metabolism of the adult lens producing an increase in its dioptric power.

Aged↗
PubMed1977

Purification and characterization of an extracellular ATPase from oviductal secretions.

Oviductal secretions include an ATPase (EC 3.6.1.3) that is transferred from the outer surface of the secretory cells to the surface of the ovulated oocyte. The enzyme has been purified and is a highly labile, very high molecular weight lipoprotein complex (greater than 4-10(6)). It consists of 47% protein and 53% lipid. Lipid composition is limited to phosphatidylcholine, phosphatidylethanolamine and sphingomyelin. The basic protein subunit has a molecular weight of 170 000. The enzyme exhibits many of the characteristics of ectoenzyme ATPase. The enzyme is Mg2+ or Ca2+ dependent; the Mg2+-ATPase has pH optima at 6.0 and 7.8 and the Ca2+-ATPase at 9.0. Substrate specificity is limited to ATP with lesser activity towards GTP, CTP, UPT and ADP. Km for ATP is 0.88 mM and the enzyme is inhibited at substrate concentrations greater than 3 mM ATP.

Adenosine Triphosphatases↗
PubMed1977

Separation of cells with different histocompatibility (H-2) antigens by passage through cellular immunoadsorbent columns.

The anti-Ig column technique of Wigzell et al. (Scand. J. Immunol. 1972. 1:75) was adapted to the separation of mouse cells with different H-2 antigens. Fractionation was achieved by a two-step procedure: first the cells were treated with anti-H-2 sera, and thereafter passed over anti-mouse Ig columns. Antibody-coated cells were selectively retained in the anti-Ig column. The method could be applied to the separation of normal mouse lymphocytes or lymphoma cells with different H-2 antigen complexes and for the selective isolation of H-2 loss variants from a mouse carcinoma/fibroblast hybrid.

Animals↗
PubMed1977

Demonstration of immunoglobulin in tumor and marginal tissues of squamous cell carcinomas of the head and neck.

Immunofluorescence (IF) techniques on cryostatcut sections of tumor tissues demonstrated that immunoglobulin was associated with cells of squamous cell carcinomas of the head and neck. The immunoglobulin was found consistently to be of the IgG class; IgM and IgA were detected also but in only one tumor sample each. The C3 component of complement was also in most tumor tissues. The immunoglobulin could be removed from the tissues by being washed with low pH glycine buffer but not with pH 7 buffer, indicating that the immunoglobulin may be in antibody-antigen complexes. All the tissues obtained from the histologically normal margins of the surgical specimens were also positive for the presence of bound immunoglobulin, whereas head and neck epithelial tissues from tumor-free control patients were negative in the IF assays. Preliminary experiments showed that IgG from patients' diluted serum and the IgG fraction isolated from patients' serum would bind to glycine buffer-eluted tumor tissue.

Antibodies, Neoplasm↗
PubMed1977

Canine kidney preservation: comparison of "intracellular", "extracellular," and high molecular weight dextran flushing solutions.

The effects of modified Collins-2 solution, adjusted lactated Ringer's solution, and a solution containing dextran 70 on kidney preservation and red blood cell (RBC) washout in dogs were evaluated. Excised kidneys were stored on ice for 24 hours and then reimplanted, at which time the contralateral kidney was removed. RBC washout from the preserved kidneys was measured at the time of initial cold flush. For 20 days after transplantation, serial measurements were made of serum creatinine, blood urea nitrogen, creatinine clearance, serum and urine osmolality, blood pH, and dog weight. No one solution cleared RBCs from the kidneys better than the other solutions. Renal function was significantly better in transplanted kidneys flushed and preserved with the Collins-2 solution than that in kidneys flushed and preserved with the other two solutions. There was generally no significant difference in function between kidneys preserved with dextran and those preserved with lactated Ringer's solution. Our findings suggest that the electrolyte composition of the flush solution may be more important than maintaining a high osmolality in the flush solution in the preservation of renal function during 24 hours' cold storage.

Animals↗
PubMed1977

Surfer's elbow.

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Adult↗