Search PubMed⌕ Search

Biomedical subjects

A Zimmermann

Publications and source records attributed to A Zimmermann.

At least 343 records · Page 19Linked to original sources
PubMed1984

[Clinical aspects and pathology of chondromatous tumors of the larynx].

Two cases of chondroma of the larynx are presented: After the first laryngofissure and excision both tumours recurred so that several operations became necessary. A transformation from chondroma to chondrosarcoma was observed based on repeated histological examinations. In the first patient pulmonary metastases and a recurrence at the tracheostomy appeared 11 years after presentation and 41/2 years after laryngectomy. In the other female patient no obvious signs of the neoplasm are present 9 years after presentation, and 4 months after the last operation for recurrence, but the prognosis remains undetermined. 101 cases of chondrosarcoma from the literature plus our 2 observations are listed and compared with 130 chondrosarcomas of the skeleton. Contrary to previous opinions, we conclude that chondrosarcoma of the larynx behaves in the same malignant manner as those of the skeleton. Some develop metastases quickly, others only after years and after at least one operation for recurrence. We could demonstrate that a detailed histological examination allows the prognosis to be assessed. Additional risk factors were also investigated. These two cases enable a better and more reliable judgement to be made of the prospective biological behaviour of chondromatous tumours of the larynx and at an earlier period of time of the process.

Aged↗
PubMed1984

Impaired neutrophil locomotion associated with hyperadhesiveness in a patient with Chédiak-Higashi syndrome.

Neutrophils from a patient with Chédiak-Higashi (CH) syndrome exhibited defective directional locomotion in a gradient of activated plasma. Further analysis of the nature of this defect showed that CH neutrophils could respond normally to stimulation with f-Met-Leu-Phe, by increasing both their motility and polarization, provided the cells were kept in suspension. Contact with the substratum resulted in the loss of both motility and polarity in the majority of cells. CH neutrophils, in contrast to normal cells, did not respond chemokinetically to f-Met-Leu-Phe. Unstimulated random locomotion of CH neutrophils was also depressed, and this correlated with increased spreading on the substratum. Our results indicate that motility, locomotion and polarisation of CH neutrophils on the substratum are depressed because of excessive adhesion.

Cell Adhesion↗
PubMed1983

beta-Nerve growth factor (beta NGF) receptors on glial cells. Cell-cell interaction between neurones and Schwann cells in cultures of chick sensory ganglia.

Receptors for beta-nerve growth factor (beta NGF), so far regarded as specific cell surface markers of certain peripheral neurones, were found to be expressed on cultured non-neuronal cells of chick embryo dorsal root ganglia (drg) (Kd beta NGF = 2 X 10(-9) M). Autoradiography revealed that binding of [125I] beta NGF was restricted to a subpopulation of the non-neuronal drg cells. Cultured embryonic skin fibroblasts, liver cells, gut cells, muscle fibroblasts, myoblasts, and myotubes, as well as macrophages and the cell lines 3T3, 3T3SV40, BHK, BHK Py, PCC3 and ND1, did not express receptors for beta NGF. Non-neuronal drg cells obtained by a procedure designed for the preparation of pure Schwann cells, as well as RN6 Schwannoma cells, were beta NGF receptor positive. The beta NGF receptor-positive non-neuronal drg cells displayed behaviour typical of Schwann cells in their interaction with drg neurones in single cell, as well as explant cultures. Three stages of neurone-Schwann cell interaction were discernible: (1) association--neurites preferentially grew over beta NGF receptor-positive non-neuronal cells; (2) cell division/alignment--beta NGF receptor-positive non-neuronal cells were induced to proliferate and aligned and elongated along neurites; (3) ensheathment--the outline of beta NGF receptor-positive non-neuronal cells and neurites merged. In drg cell cultures prepared from embryonic stages E6-E10, 25-40% of the non-neuronal cells were beta NGF receptor-positive. Later in development, from E12 onward, less than or equal to 1% of the cultured non-neuronal cells expressed beta NGF receptors.

Animals↗
PubMed1983

Liver pathology in transient neonatal hyperammonemia.

Ultrastructural investigations have been performed on two cases of transient neonatal hyperammonaemia (TNH). This newly recognized metabolic disorder is chiefly characterized by severe hyperammonaemia in the postnatal period, a comatous state, absence of abnormal organic aciduria, normal activity of urea cycle enzymes and, usually, complete recovery. The aetiology is presently unknown. Electron microscopy uncovered rather congruent alterations of hepatocyte structure, with a wide spectrum of mitochondrial lesions, an increase of autophagous bodies with organelle remnants, and changes in the excretory apparatus. Thus, in contrast to some of the hereditary disorders of the urea cycle, no specific structural changes could be found in TNH. This finding is in line with the observation of normal activities of main urea enzymes in these cases, and indicates that a different biochemical system may be pathogenetically involved in TNH.

Ammonia↗
PubMed1983

Flow fluorescence cytometry of deoxyribonucleic acid in meningiomas: studies on surgically removed tumor specimens compared with their cells in primary tissue cultures.

Flow cytometric deoxyribonucleic acid analysis was carried out in 15 meningiomas obtained surgically: two were fibroblastic; one was transitional; four were syncytial with few or no mitoses; three were angioblastic; three were syncytial containing many mitoses and focal micronecroses; one was polymorphic; and one was the papillary type. The histograms of nuclei in the surgical specimens were compared with those obtained from primary tissue culture cells prepared from tumor samples. Five diploid meningiomas had abnormal values for their 4C peaks that were also manifested by their tissue culture cells showing either slightly decreased or increased values during a 7- to 14-day period. Strong variations of the histograms were seen in the original material and in the primary tissue culture of the polymorphic tumor and the aneuploid meningioma of the papilary type. It is suspicious that in vitro proliferating meningioma tissue may be an index for a high risk of recurrence. The results of these seven cases are in contrast to eight further meningiomas of this series with more or less "indifferent" diploid histograms, thus suggesting that there could also be a scale of proliferative potentials of the different meningiomas rapidly demonstrable by means of flow fluorescence cytometry.

Cells, Cultured↗
PubMed1983

Renal tumours other than Wilms' tumour in the paediatric age group.

The non-Wilms' renal tumours in the paediatric age group may be classified according to age of onset, clinical-biological behaviour and morphology. This distinction is of practical importance because treatment and prognosis are different from those of Wilms' tumours. We report on clinical features, histological patterns, treatment and outcome of 9 cases seen between 1959 and 1981: - renal cell carcinoma (3 patients) - congenital mesoblastic nephroma (2 patients) - bone metastasizing renal tumour (3 patients) - angioleiomyolipoma (1 patient) Our experience is compared with the few reports of the same conditions in the literature.

Age Factors↗
PubMed1983

Formation of mast cell granules in cell cycle mutants of an undifferentiated mastocytoma line: evidence for two different states of reversible proliferative quiescence.

A heat-sensitive (hs, arrested at 39.5 degrees C, multiplying at 33 degrees C) and a cold-sensitive (cs, arrested at 33 degrees C, multiplying at 39.5 degrees C) cell cycle variant were isolated from an undifferentiated P-815 murine mastocytoma line. At the respective nonpermissive temperature, both the hs and the cs variant cells were reversibly arrested with a DNA content, typical of G1 phase. The cells of two cs variant subclones, when exposed to the nonpermissive temperature of 33 degrees C, formed metachromatically staining granules with an ultrastructure resembling that of mature mast cells. In addition, the cellular 5-hydroxytryptamine content underwent a marked increase, and the cells responded to compound 48/80 by degranulation as described for normal mast cells. On the other hand, in cells of two hs variant subclones, essentially no mast cell granules were detectable at either 33 or 39.5 degrees C. As previously reported, the cs cell cycle variant phenotype is expressed dominantly in heterokaryons obtained by fusing cs with wild-type cells, whereas hs cell cycle variant cells, similar to other hs mutants, were found to behave recessively under these conditions. Thus the state of proliferative quiescence induced in the cs cells at 33 degrees C is qualitatively different from the state of cell cycle arrest observed in hs cells at 39.5 degrees C and may represent a model for proliferative quiescence of differentiated cells in the intact organism.

Animals↗
PubMed1983

Extrinsic enteroureteroplasty: a piggyback technique for mid-ureteric replacement.

Replacement of the ureter was performed in 10 piglets using a serosa-lined intestinal tube. It was constructed by plication of the intestine and closing it at the antemesenteric border. The tube was then interposed to bridge a ureteral defect of variable length (4-6 cm). Information from IVU and macroscopic and microscopic results showed that the function of the interposed segments was achieved in all cases, however, with significant stenosis in the absence of adequate postoperative splinting in 5 cases, and neomucosa - similar to uroepithelium in the beginning and a complete urothelium after 3 months - covered the interpositioned tube.

Animals↗
PubMed1983

Crawling-like movements, adhesion to solid substrata and chemokinesis of neutrophil granulocytes.

We analysed the chemokinetic actions of fMet-Leu-Phe (FMLP) and human serum albumin (HSA) on preparations of human neutrophils. Our work focused on the extent to which these agents, individually or in combination, affected locomotion by inducing changes in motile activity and/or the degree of cellular adhesion to the substratum. Because polymorphonuclear leucocyte (PMN) preparations derived from different individuals were found to exhibit broad variations in their basal motility and level of adhesiveness to glass substrata, it became of major interest to clarify mechanisms responsible for the reported variations in neutrophil responses to FMLP. We performed detailed studies on three separate PMN preparations, which differed in terms of their basal motility and adhesiveness. The unequivocal findings that emerged from this study were as follows: (1) HSA did not stimulate or modify motility of cells in suspension. Its orthokinetic effect was exclusively due to its capacity to decrease adhesion to the substratum. It prevented immobilization of motile cells that made contact with the substratum. (2) The orthokinetic effect of FMLP was primarily due to its effects on motility, but there were also secondary effects on spreading and adhesion. The peak chemokinetic activity of FMLP occurred at 10(-8)M, when measured by the two-filter count method and this was correlated with the percentage of polarized neutrophils in suspension. (3) The response to HSA and/or FMLP depended on the basal activity of the cells. If the cells were non-motile and spherical, HSA had no chemokinetic effect. Such an effect was only observed with cells that were initially motile. In contrast, the stimulatory effect of FMLP on motility and locomotion was marked in cases where basal motile activity of the neutrophils was low. If the basal motile activity was high, motility and locomotion could not be stimulated further. Therefore, the chemokinetic effects of HSA and FMLP can be predicted when one knows the basal motile activity of the neutrophils. The variable effect of FMLP on adhesion may also contribute to the regulation of locomotion. These findings help to explain earlier conflicting reports on the chemokinetic effect of FMLP. (4) FMLP can elicit simultaneous changes in motility and adhesiveness, which may have synergistic or antagonistic effects on locomotion. (5) The average speed of the whole neutrophil population is essentially regulated by changes in the proportion of locomoting cells and not, or to a lesser extent, by changes in the speed of the locomoting subset.(ABSTRACT TRUNCATED AT 250 WORDS)

Cell Adhesion↗
PubMed1983

[Importance of impulse cytophotometry in the diagnosis and prognosis of prostate cancer].

In 102 cases with hypertrophy and 73 patients with carcinoma of the prostate, all histologically proven, fine-needle biopsies were done and the obtained material investigated by conventional cytology as well as by flow-through cytophotometric determination of DNA content of the nuclei. In prostatic cancer the DNA determinations showed less diagnostic precision than conventional cytology. However as an additional diagnostic procedure the flow-through cytophotometry was able to improve the results of cytologic diagnostic for 10% in all cases with histologically proven cancer of all grades. In hypertrophy of prostate, the exclusion of carcinoma could be done by DNA determination with higher security than by conventional cytology. Flow-through cytophotometry is therefore considered a useful additional test in primary diagnostic and grading of prostatic cancer. In addition it is a method to evaluate the efficacy of drug therapy.

Flow Cytometry↗
PubMed1983

[Unusual forms of retroperitoneal fibrosis].

The numerous forms of secondary retroperitoneal fibrosis which do not belong to Ormond's disease are discussed and some rare iatrogenic clinical pictures described. For the first time polyvinylpyrrolidone granulomas, causing obstructive renal failure are reported.

Aged↗
PubMed1983

Congenital nephrotic syndrome: clinico-pathological heterogeneity and prenatal diagnosis.

During the last five years, we have observed four families in which siblings were affected with the congenital nephrotic syndrome (CN). Clinically, all patients, with one exception, could barely be differentiated from classical examples of CN of the Finnish Type (CNF). Morphologically, however, each family showed a different type of CN, although the appearance in siblings was identical. This morphological heterogeneity, makes it likely that CN in a population outside Finland is the result of an unknown number of autosomal recessive mutations at different gene loci. In an attempt to diagnose CN prenatally by means of measurements of the concentration of alpha-fetoprotein in amniotic fluid, we found two false normal results, one in a patient with histologically confirmed CNF, another in a patient with CN, histologically non Finnish Type. Thus the results of prenatal diagnostic procedures for these disorders have to be reevaluated.

Child, Preschool↗
PubMed1982

[Development of small intestinal neomucosa].

Under certain conditions epithelium grows on the peritoneal surface. By folding the colon of the rat the authors constructed a seromuscular tunnel of which the two ends were sutured to the transsected ileum, making normal small bowel passage possible. 6-12 weeks after the operation a pouch was found on which a one-layer cylindric epithelium had developed showing evidence of disaccharidase activity. Clinically the model could be used to enlarge the mucosal surface of the remaining small bowel following extensive resection.

Animals↗
PubMed1982

Unusual pulmonary vascular lesions after intravenous injections of microcrystalline cellulose. A complication of pentazocine tablet abuse.

We present the morphological features of a case of fatal pulmonary granulomatosis from illicit intravenous injections of microcrystalline cellulose derived from pentazocine tablets. Extensive foreign body granulomas were found in the lumena and walls of pulmonary vessels and in the pulmonary interstitium. Previously unreported gaps containing foreign material were found in the walls of medium-sized muscular pulmonary arteries. This peculiar finding is discussed in the light of the possible mechanisms involved in the removal of embolized foreign material.

Adult↗
PubMed1982

Congenital "histiocytoid" cardiomyopathy: evidence suggesting a developmental disorder of the Purkinje cell system of the heart.

The so-called "histiocytoid cardiomyopathy" is an unusual cardiac disorder of infancy and childhood, characterized by the presence of numerous foamy, lipid-containing cells between the endocardium and the striated myocardial cells of the left ventricle and the interventricular septum. The disease usually affects females, the clinical picture being dominated by severe disturbances of conduction. The original designations of the disorder stem from the morphological resemblance of the foamy cells to lipid-laden histiocytes. However, subsequent investigations have shown these cells to contain myofibrils interposed with Z lines. It has, therefore, been suspected that the leading cell population might be related to the myocardium. Using a histochemical method for the demonstration of cholinesterase activity in the foamy cells, we present evidence that "histiocytoid" cardiomyopathy may in fact correspond to a maldevelopment of the Purkinje cell system of the heart.

Cardiomyopathies↗