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Biomedical subjects

A Tsukise

Publications and source records attributed to A Tsukise.

50 records · Page 3Linked to original sources
PubMed1987

Histochemical observations on the apocrine glands of the scrotal skin of the cat and dog.

Enzyme and carbohydrate histochemical methods were used to study the secretory activity of the apocrine glands of the scrotal skin of the cat and dog. The typical activity spectra of the different oxidative and hydrolytic enzymes investigated indicated high metabolic rates within the secretory cells. The carbohydrate histochemical differentiation revealed mostly neutral and partly acidic glycoproteins, with only small amounts of sialic acid, in the secretory cells and the luminal secretion of the glands. The PO-lectin-DAB procedures applied demonstrated that the following saccharide residues were dominant within the neutral glycoproteins present: alpha-D-glucose, alpha-D-mannose, beta-D-galactose, N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine.

Animals↗
PubMed1984

Complex carbohydrates in the secretory epithelium of the goat prostate.

The complex carbohydrates in the epithelium of the goat prostate were studied with a battery of light microscopical histochemical methods that included peroxidase-labelled lectin-diaminobenzidine (PO-LT-DAB) procedures. The secretory epithelial cells were found to be of three types: (a) mucus-producing cells, (b) protein-secreting cells, and (c) cells intermediate between (a) and (b). All these cell types contained variable amounts of neutral and acidic complex carbohydrates. The histophysiological significances of these substances in the glandular cells are discussed with special reference to the function of the accessory sex glands.

3,3'-Diaminobenzidine↗
PubMed1983

Histochemistry of complex carbohydrates in the hairy skin of the domestic pig.

The hairy skin from eleven body areas of the domestic pig has been studied by means of a series of selected methods for the detection of complex carbohydrates, including peroxidase-labelled lectin-diaminobenzidine procedures. The results obtained were most distinctive in the areas where the skin accessories were involved. The complex carbohydrates in the epidermis appear to be acidic and neutral glycoproteins containing a small amount of sialic acid residues. The dermal connective tissue fibres revealed positive reactions for neutral and acidic glycoproteins with notable amounts of sialic acid residues, and for proteoglycans. In different parts of the hair follicle, neutral and acidic glycoproteins were also present, most being restricted to the connective tissue sheath and the dermal papilla. In the lower regions of the outer epithelial root sheath the epithelium contained a large amount of glycogen. The sebaceous glands reacted very weakly for neutral and acidic glycoproteins. The apocrine glands exhibited mostly neutral but also acidic glycoproteins in the secretory cells and the lumenal secretion, with substantial amounts of various saccharide residues.

Animals↗
PubMed1983

Histochemistry of complex carbohydrates in the skin of the pig snout, with special reference to eccrine glands.

The histochemistry of carbohydrates has been studied in the skin of the pig snout with selected methods of light microscopy including peroxidase-labelled lectin-diaminobenzidine (PO-LT-DAB) procedures. In the snout skin the dark secretory cells and the luminal secretion of the eccrine glands contained considerable amounts of neutral glycoproteins, but only a very small amount of acidic ones. It was possible to demonstrate glycogen in the secretory cells of these glands. The other skin structures of the snout also showed positive reactions for complex carbohydrates. Most remarkable were stronger reactions of intercellular substances among the spinosum cells, particularly following the PO-LT-DAB procedures, which demonstrated such saccharide residues as beta-D-galactose and N-acetyl-D-glucosamine.

Animals↗
PubMed1982

Carbohydrates in the epithelium lining the seminal vesicle of the rat as studied by histochemical methods of light and electron microscopy.

In the epithelium lining the seminal vesicle of the rat, carbohydrates have been studied by means of histochemical methods of light and electron microscopy. In light microscopy, the free surface and granules of different sizes in the distal cytoplasm of the epithelial cells and lumenal masses of secretions were shown to exhibit positive reactions for carbohydrates with 1,2-glycol and acidic groups and sialic acid residues. In electron microscopy, the surface coat of the plasma membrane, certain elements of the Golgi complexes, dense cores of secretory granules and glycogen particles in the epithelial cells were found to exhibit positive reactions for carbohydrates with 1,2-glycol groupings. The correlated structures and functions of the carbohydrate-containing components have been discussed in the light of the known physiology of the seminal vesicle in the rat.

Animals↗
PubMed1981

The histochemistry of complex carbohydrates in the scrotum of the boar.

In the scrotal skin of the boar, the histochemistry of complex carbohydrates has been studied by means of a series of selected methods of light microscopy. The epidermis of the scrotal skin was found to contain neutral and acidic complex carbohydrates with different saccharide residues. The secretory epithelial cells and secretory substances of the saccular apocrine sweat glands contained sulfated, other acidic and neutral complex carbohydrates, whereas the secretory epithelial cells and secretory substances of the tubular apocrine sweat glands involved largely neutral complex carbohydrates. The two types of complex carbohydrates from the both glands were shown to contain commonly substantial amounts of various saccharide residues but were devoid of notable amounts of sialic acid residues. In addition, complex carbohydrates in the smooth muscle cells were reacted for relatively small amounts of saccharide residues. From the present results, the histophysiological significance of complex carbohydrates in the particular histologic structures of the scrotum have been discussed with special reference to the functions of the skin in the boar.

Animals↗
PubMed1981

The histochemistry of complex carbohydrates in the epithelium lining the ventral prostate of the rat.

In the epithelium lining the ventral prostate of the rat, complex carbohydrate-containing structures have been studied by means of both light and electron microscopic histochemical methods. According to light microscopy, the free surface and granules of different sizes in the distal cytoplasm of the epithelial cells were found to exhibit positive reactions for complex carbohydrates with 1,2-glycol and acidic groups and sialic acid residues. In addition, secretory substances within the glandular lumen were shown to exhibit positive reactions for similar groups and saccharide residues of complex carbohydrates. In electron microscopy, the surface coat of the plasma membrane, certain elements of the Golgi apparatus and lysosomal dense bodies were found to exhibit positive reactions for glycoproteins with 1,2-glycol groupings. The histophysiological significances of the carbohydrate-containing structures have been discussed with special reference to the known physiological functions of the prostate in the rat.

Animals↗
PubMed1979

Histochemistry of carbohydrates in the epithelium lining the bulbourethral gland of the rat.

The histochemistry of carbohydrates has been studied in the epithelium lining the bulbourethral gland of the rat by means of light- and electron-microscopic methods. The results obtained show that the cytoplasms of the epithelial cells contain neutral and acidic carbohydrates. The neutral carbohydrates exhibited positive reactions with periodic acid-Schiff and periodic acid-thiosemicarbazide-silver proteinate, whereas the acidic carbohydrates reacted positively with alcian blue (AB; pH 1.0 and 2.5) and dialyzed iron. Most neutral carbohydrates were found to be glycoproteins localized within the secretory granules. The acidic carbohydrates consist of at least two types, AB (pH 1.0)-reactive sulfated and AB (pH 2.5)-reactive nonsulfated carbohydrates; most nonsulfated carbohydrates were determined to be sialic acid. The acidic carbohydrates were also localized within the secretory granules.

Animals↗
PubMedDate not supplied

Secretory glycoconjugates in the epithelium of the goat prostate.

Secretory glycoconjugates in the epithelium of the goat prostate were investigated with various electron microscopical histochemical methods that involved periodic acid-thiocarbohydrazide-silver proteinate, colloidal gold-labelled lectin and related procedures. The three types of cells in the epithelium previously differentiated with the light microscope were substantiated: mucus-producing cells, protein-secreting cells and cells intermediate between both types. These three cell types contained varying amounts of neutral glycoconjugates; the histochemical nature of these carbohydrates was determined with particular emphasis upon the histophysiological functions of the accessory sex glands.

Animals↗
PubMedDate not supplied

Histochemical analysis of glycoconjugates in the eccrine glands of the raccoon digital pads.

The distribution and selectivity of complex carbohydrates in the eccrine glands of the digital pads in the North American raccoon (Procyon lotor) were studied using light and electron microscopic histochemical methods, particularly lectin histochemistry. In the eccrine glands, the dark cells exhibited neutral and acidic glycoconjugates with different saccharide residues (alpha-L-fucose, beta-D-galactose, beta-N-acetyl-D-glucosamine and N-acetyl-neuraminic acid); the clear cells contained numerous glycogen particles and showed a distinct reaction of alpha-L-fucose. The presence of complex carbohydrates with various terminal sugars was evident in the excretory duct cells. In addition, beta-D-galactose and N-acetyl-neuraminic acid residues were mainly observed in the luminal secretion. The glycoconjugates produced by the eccrine glands of the raccoon digital pads may protect the epidermis against physical damage or microbial contamination. In this way, the normal functioning of the sensory apparatus of the foot pads is ensured.

Animals↗
PubMedDate not supplied

Morphological, histochemical and immunohistochemical characterization of secretory production of the ciliary glands in the porcine eyelid.

In addition to performing general histology and cytology of the ciliary glands of the miniature pig, we studied the localization of glycoconjugates and beta-defensins in these glands with the use of carbohydrate histochemical and immunohistochemical methods. The secretory cells of the glands were equipped with non-homogeneous secretory granules, a well-developed Golgi apparatus and rough endoplasmic reticulum. The secretory epithelium and luminal secretion of the glands contained large amounts of neutral and acidic glycoconjugates with various saccharide residues (alpha-L-Fuc, beta-D-Gal, alpha-D-GalNAc and sialic acid). The sebaceous glands and tarsal glands also exhibited positive reactions to most of the histochemical methods. Additionally, the antimicrobial peptide group of beta-defensins was demonstrated to be products of the ciliary glands, as well as the sebaceous glands and tarsal glands. The results obtained are discussed with regard to the specific function of the ciliary glandular secretions. These secretory products may be related to the moistening and general protection of the skin surface of the eyelid and ocular surface.

Animals↗