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Biomedical subjects

A Tsukise

Publications and source records attributed to A Tsukise.

At least 37 records · Page 2Linked to original sources
PubMed1995

Effects of splenectomy on luteolysis in pseudopregnant rabbits.

The effects of splenectomy on luteolysis in pseudopregnant rabbits were observed. Pseudopregnancy was induced in the rabbits by mating with vasoligated mature males and injection of human chorionic gonadotrophin (D0). In these rabbits, the concentration of serum progesterone increased for 7 days after the induction of pseudopregnancy. In the control group (sham-operation), the concentration of serum progesterone returned to the level of the pre-ovulatory levels by 14 days of pseudopregnancy. On the other hand, in the splenectomized group, in which splenectomy was performed at D7, the serum progesterone concentration was maintained at the level of the functional luteal phase (D7) at least until 21 days of pseudopregnancy. These findings indicate that the spleen is concerned with luteolysis of the rabbit.

Animals↗
PubMed1995

Effects of TNF-alpha injection into the ovarian parenchyma on luteal blood vessels in rabbits.

It has been reported that tumor necrosis factor-alpha (TNF-alpha) derived from luteal macrophage is concerned with luteolysis. In the present study, to evaluate the correlation between TNF-alpha and regression of luteal blood vessels, recombinant human TNF-alpha (rh-TNF) was injected into the parenchyma of pseudopregnant rabbit ovaries. These injections were performed on day 7 of pseudopregnancy (functional luteal phase). Only Mg++ and Ca++ free phosphate buffer saline (PBS-) as the solvent was injected in the control group. Estimations of conditions in the luteal vessels after the injections was performed by observations of luteal vascular corrosion casts under a scanning electron microscope. Concentrations of serum progesterone before or after the injection were also assayed. In the control group, no change in the structure of luteal vessels was observed after PBS-injection, but regressing blood vessels with strictures, obstructions and rugged surfaces on the vessels were observed, and also concentrations of serum progesterone decreased noticeably after rh-TNF injection. These findings suggest that TNF-alpha plays a role in angiolysis through luteolysis in the rabbit corpus luteum.

Animals↗
PubMed1994

Immunohistochemical characterization of a stage-specific antigen during oogenesis and spermatogenesis recognized with monoclonal antibody.

In order to detect and characterize novel molecules which function in oogenesis, immunohistochemical study using a monoclonal antibody (MAb) raised against oocytes of Xenopus laevis was carried out. The distribution of the detectable molecule with MAb X-80, which specifically reacts with Xenopus previtellogenic oocytes; stages I and II (Dumont, 1972), in particular was analyzed. The hamster, mouse, chick, quail, Caenorhabditis elegans and Lilium longiflorum were also examined. Interestingly MAb X-80 bound not only in Xenopus oocytes but in its secondary spermatocytes and spermatids. Furthermore, MAb X-80 gave a similar staining pattern in the Lilium and other, although the stage when the positive reaction is detectable is different in the male and female germ cells.

Animals↗
PubMed1994

Localization of ZP3 mRNA in mouse ovary by non-radioactive in situ hybridization with digoxigenin-labelled cDNA.

In situ hybridization using digoxigenin-labelled cDNA has dual advantages of having high structural resolution and high sensitivity and avoiding problems associated with the handling of radioactive materials. We have used this technique to detect and locate mRNA for mouse ZP3 protein in paraffin sections of fixed ovaries of newborn and adult mice. Mouse ZP3 mRNA was restricted to the cytoplasm of oocytes. After day 16, ZP3 mRNA expression was increasingly restricted to the cytoplasm of the oocyte. Previous work on the distribution of ZP3 mRNA was confirmed and we were able to show that using non-isotopic in situ hybridization, sensitive and accurate cellular localization was feasible in paraffin embedded mouse ovary.

Alkaline Phosphatase↗
PubMed1992

Histochemical studies on the dependence of secretory function of the major vestibular gland (Bartholin's gland) on ovarian steroid hormones in the cat.

The dependency of the secretory function of the feline major vestibular gland (Bartholin's gland) on the ovarian steroid hormones was studied histochemically. After estrogen (estradiol-17 beta or diethylstilbestrol), progesterone or a combination of these was administered to cats which were previously ovariohysterectomized, the major vestibular glands were removed, embedded in paraffin, sectioned, and stained by alcian blue, periodic acid-Schiff or peroxidase-labeled lectin (peanut agglutinin and wheat germ agglutinin). The vividly positive reactions to the stainings applied were observed in the secretory epithelial cells of the major vestibular glands in the estrogen treated animals. The dependency of the secretory function of the major vestibular glands on the estrogen was demonstrated in this study.

Animals↗
PubMed1991

Appearance of lectin binding affinity to the zona pellucida during hamster oocyte maturation.

In our previous study, a drastic change in terminal saccharides of glycoconjugates of the hamster zona pellucida associated with oocyte maturation was observed using light microscopic methods of lectin cytochemistry. To understand the mechanism of this change, in the present study, the correlation between the cytochemical appearance of saccharide residues in the zona pellucida and nuclear maturation was examined. Immature hamsters were treated with PMSG and hCG to induce follicular development and ovulation. The animals were euthanized 0 to 26 hrs. after the injection of PMSG or 0,1,2,3,4,5,7,9 or 11 hrs. after the injection of hCG, and ovaries were dissected out, fixed, paraffin embedded and sectioned serially. Every other paraffin section was stained with hematoxylin to observe the status of nuclei and to classify follicular growth and only the fully developed preovulatory follicles were examined in experiments. The peroxidase-labelled lectin-diaminobenzidine procedure was applied to sections. The lectins employed were WGA, SBA, MPA, UEA-I, LotusA and AAA. Germinal vesicle breakdown was observed within 3 hrs. after the administration of hCG. A positive reaction of WGA, SBA or MPA for zonae pellucidae in the fully developed preovulatory follicles appeared 1 hr. after hCG injection, and remained so for the next 10 hrs. UEA-I, Lotus A and AAA reactions were negative for all of the zonae pellucidae observed. The data indicate that the synthesis of saccharide residues such as GlcNAc and GalNAc forming zona components in the follicles is not triggered by germinal vesicle breakdown.

Animals↗
PubMed1990

An efficient histochemical method for deoxyribonucleic acids using a silver enhancement procedure.

An efficient histochemical method has been worked out for the demonstration of deoxyribonucleic acids (DNA) with light microscopy. This involves a hot hydrochloric acid-thiocarbohydrazide-silver proteinate sequence followed by a physical development procedure. When applied to tissue sections from rabbit organs such as the spleen, cerebellum and testis, the new method has given excellent discrimination of nuclear DNA in all cells examined. Thus, the method is higher in efficiency and visibility of reaction products than the traditional hot HCl-Schiff (Feulgen) method used hitherto. Its specificity is also high.

Animals↗
PubMed1990

Glycoconjugate histochemistry of the prostate gland in the pig.

In the porcine prostate, glycoconjugates elaborated and released by the glandular cells have been studied by means of a series of histochemical methods of light microscopy. The employed methods included several routine procedures in combination with digestion by carbohydrate-degrading enzymes and peroxidase-labelled lectin diaminobenzidine procedures. In the glandular tissues, the particular glycoconjugates were shown to be secretory glycoproteins which contained vicinal diol, sulphate, and carboxyl groups and were provided with different saccharide residues such as alpha-D-mannose, alpha-D-glucose, lactose, N-acetyl-D-galactosamine, beta-D-galactose, alpha-L-fucose, N-acetyl-D-glucosamine, and N-acetyl-neuraminic acid. These glycoconjugates were, likewise, found to exhibit positive reaction for proteins. The present histochemical results have been discussed with special reference to the histophysiological functions performed by the prostate gland in the pig.

Animals↗
PubMed1989

Histochemistry of glycoconjugates in the skin of the bovine muzzle, with special reference to glandular structures.

The distribution of glycoconjugates in the muzzle of young adult Holstein cows has been studied by means of selected light-microscopic histochemical methods, including lectin histochemistry. In the skin layers, strong reactions were confined to intercellular substances in between the cells of the vital epidermis, exhibiting neutral glycoconjugates mainly with alpha-D-galactosyl and N-acetyl-D-galactosaminyl residues. In the nasolabial glands, distinctly positive staining for neutral glycoproteins with various saccharide residues (alpha-D-galactose, alpha-N-acetylgalactosamine, D-galactose-beta(1----3)D-N-acetylgalactosamine, beta-D-galactose), and for smaller amounts of acidic glycoconjugates, was found in the secretory cells and the luminal secretion. The cells of the excretory duct system showed weak to moderate reactions (alpha-D-galactose, beta-D-galactose), only the collecting ducts reacted positively for acidic glycoproteins with sialyl residues. The results obtained are discussed in view of muzzle function, with special reference to the salivary nature of the secretion of bovine nasolabial glands.

Animals↗
PubMed1989

Lectin histochemical aspects of vascular development in fetal mammalian skin.

The study describes lectin reactivity of the developing vascular network in fetal mammalian skin. Skin samples were taken from fetuses of different gestational age of the pig, the goat and the cat. Clearly positive, endothelial lectin staining of capillaries and smaller vessels could only be obtained during mid gestation. The sequence of lectin reactivity, demonstrating: 1st alpha-D-N-acetylglucosamine and N-acetyl-alpha-D-galactosamine, 2nd alpha-D-galactose and sialic acid, and 3rd alpha-L-fucose, sialic acid and probably alpha-D-mannose/alpha-D-glucose residues, emphasizes different stages of cell surface coat as well as of cell membrane development. Thus, the typical outgrowth period of developing integumental blood vessels is reflected histochemically.

Animals↗
PubMed1988

Histochemical demonstration of zinc in rat epididymis using a sulphide-silver method.

We studied the histochemical distribution of zinc in rat epididymis using a sulphide-silver method. In the supranuclear cytoplasm of the principal cells that line the epididymis of rats, varying amounts of sulphide-silver-reactive zinc were visualized. In adult mating rats, significant amounts of zinc were found in the proximal portion of the epididymis, whereas in non-mating, mature and immature young rats, this heavy metal was most prominent in the distal portion of this organ. In all of the rats studied, zinc was sparsely distributed in the intermediate portion of the epididymis. From these results, it can be assumed that the zinc present in the epithelial lining of rat epididymis plays an important role in the maturation of spermatozoa. The present results represent a useful contribution to our understanding of the functional morphology of rat epididymis.

Animals↗
PubMed1988

The cytochemistry of glycoconjugates in the planum nasolabial gland of the goat as studied by electron microscopic methods.

In the planum nasolabial glands of the goat, glycoconjugates of glandular and duct cells have been studied by means of a series of electron microscopic cytochemical methods. In the glandular cells glycoconjugates with vicinal diol groupings were present in secretory granules, certain elements of the Golgi complex, lysosome-like dense bodies, the surface coat of the plasma membrane, the majority of intracellular cytomembranes, glycogen particles and the basal lamina. In duct cells, glycoconjugates with the same properties were localized in similar ultrastructures, except for secretory granules, which were not detected in these cells. By lectin cytochemistry, glycoconjugates in glandular cell secretory granules contained a variety of saccharide residues such as alpha-D-mannose, alpha-D-glucose, N-acetyl-D-glucosamine and alpha-L-fucose. The cytochemical properties of the secretory glycoconjugates are discussed in relation to the physiological functions performed by the planum nasolabial glands in the goat.

Animals↗
PubMed1988

Histochemistry of glycoconjugates in the goat nasolabial skin with special reference to eccrine glands.

The histochemistry of glycoconjugates in the nasolabial skin of the goat has been studied by means of a series of selected methods of light microscopy. The epidermis of the nasolabial skin was found to contain neutral and acid glycoconjugates with different saccharide residues. The secretory epithelial cells and secretory substances of the sebaceous glands contained primarily neutral glycoconjugates, whereas those of the apocrine glands involved largely strongly acidic and neutral glycoconjugates. In the epithelial cells and secretory substances of the nasolabial eccrine glands, glycoconjugates involved were characteristically strongly neutral but weakly acidic in nature. From the present results, the histophysiological significance of glycoconjugates in the particular histologic structures of the nasolabial skin has been discussed with special reference to the functions of this particular skin type in the goat.

Animals↗
PubMed1987

Histochemistry of glycoconjugates in the secretory epithelium of the goat bulbourethral gland.

To investigate the histochemical nature of the secretory epithelium lining the goat bulbourethral gland, glycoconjugates contained in these secretory epithelial cells have been studied by means of light- and electron-microscopic histochemistry. The methods employed involved a series of conventional staining and peroxidase-labeled lectin diaminobenzidine procedures together with combined selective methods such as digestion with enzymes. According to the results obtained in the present work, the secretory cells of the goat bulbourethral gland can be grouped into two types: cells with glycoconjugate-rich granules and those with less amounts of glycoconjugates. The gland is at least dual in the nature of its secretory activities.

Animals↗