Search PubMed⌕ Search

Biomedical subjects

A Tracqui

Publications and source records attributed to A Tracqui.

At least 91 records · Page 5Linked to original sources
PubMed1989

Adverse events of anti-glaucoma beta-blockers: presentation of an original HPLC determination procedure.

A HPLC method is described for the identification and quantification in plasma and urine of beta-blockers (betaxolol, carteolol, metipranolol, and timolol) topically used in the therapy of glaucoma. An extraction method is presented using pindolol as an internal standard. Separation is performed on an RSIL CN 10 microns column with a mobile phase of water/acetonitrile/pH 3.0 0.1 M NaH2PO4 buffer solution (60:30:10, v/v). The lower detection limits were found to be 4 to 27 ng/ml. This method is simple, rapid and sensitive; moreover, it allows the determination of 8 other beta-blockers.

Adrenergic beta-Antagonists↗
PubMed1989

Subnanogram GC/NPD method for the determination of sparteine in biological fluids.

A gas chromatographic method is presented for identification and quantification of sparteine in biological fluids using cyclizine as an internal standard. No derivation is necessary and after a single alkaline extraction, GC analysis of sparteine is achieved in less than 8 min. A subnanogram limit of detection is allowed by the use of a nitrogen phosphorous detector (NPD). This method is simple, reproducible, selective, and applicable in both clinical and pharmacokinetic studies.

Chromatography, Gas↗
PubMed1988

Fatal meprobamate self-poisoning.

A case involving a suicidal overdose resulting from the ingestion of 90 tablets (400 mg) of meprobamate is presented. The drug was quantified using a gas chromatograph equipped with a flame ionization detector (FID). While the blood level was 204.6 micrograms/ml, the maximum concentration was found in the heart (708 micrograms/g), confirming the cardiac toxicity of meprobamate. Other drugs were not detected.

Adult↗
PubMed1988

Fatal chloroquine self-poisoning.

High performance liquid chromatography was employed to quantify chloroquine in biological fluids and tissues in a death attributed to chloroquine overdose. The blood concentration of chloroquine was 16.71 mg/l. Results are discussed in the light of the existing literature.

Adult↗
PubMed1988

Simultaneous determination of glafenine and floctafenine in human plasma using high-performance liquid chromatography.

A high-performance liquid chromatography (HPLC) assay has been developed for the determination of glafenine and floctafenine in plasma. Drugs are extracted with ethyl acetate from alkalinized samples using antipyrine as an internal standard. After evaporating the solvent to dryness, the residue is removed and analyzed using a microPorasil 10 micron column with a mobile phase of n-hexane and ethanol (80/20, v/v). The drugs are detected at 240 nm. The lower detection limits are 1.993.10(-2) mumol/l and 3.138.10(-2) mumol/l for glafenine and floctafenine, respectively. The method is simple, rapid and very sensitive. It is the first HPLC method for the determination of floctafenine.

Antipyrine↗
PubMed1988

Simultaneous determination of mefloquine and chloroquine in biological fluids using high-performance liquid chromatography.

A high-performance liquid chromatography (HPLC) method for the simultaneous determination of mefloquine and chloroquine in biological fluids is described. Methaqualone is used as an internal standard. After a single-step extraction, achieved from alkalinized samples with diethyl ether, a separation is obtained using a mu-Bondapak phenyl column with a mobile phase of acetonitrile and sodium acetate buffer. Detection is done at 305 nm. The lower detection limits are 3 and 10 micrograms/l for mefloquine and chloroquine, respectively. Thus, this method appears to be simple, rapid, very sensitive and applicable in pharmaco-clinical, toxicological and forensic analyses.

Antimalarials↗
PubMedDate not supplied

A specific HPLC method for determination of beta-blockers topically used in ophthalmological diseases.

A HPLC method is presented for the identification and quantification in plasma and urine of beta-adrenergic receptor antagonists (betaxolol, carteolol, metipranolol, and timolol) commonly prescribed in ophthalmology. An extraction method is described using pindolol as an internal standard. An RSIL 10 micron column was used. The lower detection limits of the beta-blockers were found to be 4-27 ng/ml. This method is simple, rapid and sensitive; moreover, it allows the determination of 8 other beta-blockers.

Administration, Topical↗
PubMedDate not supplied

Toxicology and fly larvae on a putrefied cadaver.

Toxicological analyses on a putrefied cadaver are sometimes difficult to achieve, due to the absence of blood and/or urine. In this study, morphine and phenobarbital were simultaneously identified and assayed in several tissues of a putrefied cadaver and in the fly larvae of Calliphoridae found on the corpse.

Adult↗
PubMedDate not supplied

Analysis of opiates in fly larvae sampled on a putrefied cadaver.

Toxicological analyses on a putrefied cadaver are sometimes difficult to achieve, because of the absence of blood and/or urine. Drugs present in a decomposing corpse may be identified through analysis of maggots feeding off it. In this study, morphine and codeine were simultaneously identified and assayed in blood and bile of a putrefied cadaver and in the fly larvae of Calliphoridae found on the corpse.

Animals↗
PubMedDate not supplied

Fatal intoxication by dextromoramide: a report on two cases.

Two cases involving an overdose resulting from the abuse of dextromoramide are presented. The drug was quantified with a gas chromatograph equipped with a nitrogen phosphorous detector (NPD). The whole blood dextromoramide concentrations were 984.3 ng/mL for case 1 and 871.1 ng/mL for case 2. No correlations could be established with the postmortem levels in blood and in bile.

Adult↗
PubMedDate not supplied

Toxicological findings in a fatal case of acebutolol self-poisoning.

A fatal case of acebutolol self-poisoning is presented. After single-step liquid-liquid alkaline extraction, acebutolol was identified by using an HPLC/DAD screening procedure. By means of a specific HPLC method, acebutolol was then quantified in a large range of postmortem samples. The blood acebutolol concentration was 34.7 micrograms/mL. The tissue distribution of the drug is discussed in the light of the existing literature.

Acebutolol↗
PubMedDate not supplied

Nonfatal prolonged overdosage of pyrimethamine in an infant: measurement of plasma and urine levels using HPLC with diode-array detection.

A nonfatal case of accidental prolonged overdosage of pyrimethamine (10 times the usual dose for 10 days) in a seven-week-old infant treated for congenital toxoplasmosis is presented. The drug was identified and quantitated in plasma and urine using an HPLC technique with diode-array detection. Initial plasma concentration of pyrimethamine was 6.22 micrograms/mL. Results are discussed in light of the existing literature.

Chromatography, High Pressure Liquid↗