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Biomedical subjects

A Tosca

Publications and source records attributed to A Tosca.

49 records · Page 3Linked to original sources
PubMed1982

Anhidrotic ectodermal dysplasia. Therapeutic attempts.

In 2 patients with anhidrotic ectodermal dysplasia, we were able to show that the hypoplastic eccrine glandular elements may give rise to normal eccrine glands both anatomically and functionally, after repeated local application of acetylcholine. The perspectives of our findings for the therapeutic management of the anhidrotic ectodermal dysplasia are discussed and special attention is paid to the genetic background of the defect as well as the possible mode of action of acetylcholine.

Acetylcholine↗
PubMed1981

Evaluation of various antigenic substrates for the detection of antinuclear antibodies.

The present study deals with the evaluation of some substrates for the detection of nuclear antibodies by the indirect immunofluorescence method. We have examined white male mouse liver imprint, white female pregnant mouse liver imprint, compound substrate consisting of male mouse liver, kidney and gastric wall sections, skin sections from psoriatic patients, skin sections from squamous cell carcinomata, male rabbit spleen imprint, skin sections from healthy adults people, skin sections from basal cell epitheliomata, skin sections from patients with lichen planus, male mouse spleen imprint and male guinea pig spleen imprint. To evaluate the substrates, we have employed 4 selected sera from patients with collagen disease. It was observed that some types of antinuclear antibodies demonstrate greater affinity for certain substrates and the fluorescent nuclear pattern might change on serial dilutions. From all the substrates studied, the white male mouse liver imprint and the male rabbit spleen imprint were considered as the most efficient for routine purpose as they are both sensitive and easily obtainable.

Animals↗
PubMed1980

Antigenic alterations in tumors of epidermal origin.

One hundred tumors of epidermal origin were studied by means of indirect immunofluorescence technique with sera from patients with proven pemphigus and bullous pemphigoid. We found that in tumors of epidermal origin, there was a loss of antigenicity of the intercellular substance and basement membrane; this was minimal for benign tumors, greater for premalignant tumors, and very clear for malignant tumors. We also found a gross correlation between the histologically proven malignancy of squamous cell carcinoma and the grade of antigenic loss; both for the intercellular substances and for the basement membrane antigens. However, this did not yeild definite results in the group with basal cell carcinoma. With regard, especially, to the intercellular substance antigen, it seemed to be completely independent of histologic type of basal cell carcinoma. We found that the duration of the epidermal tumor did not correlate with the percentage of intercellular substance and basement membrane antigenic loss. This suggested that the antigenic loss was an early feature of neoplastic behavior. The deeper parts of the malignant tumors in the dermis showed a lower percentage of antigenicity for both antigens.

Antigens, Neoplasm↗
PubMed1980

Immunological study of keratoacanthomas.

Twelve patients with keratoacanthoma were studied to assess the role and importance of immunological factors in tumor regression. Direct immunofluorescence was determined with immunoglobulins, complement (C3), and fibrin to estimate the deposition of these factors in the lesion area. Indirect immunofluorescence was also undertaken using pemphigus and bullous pemphigoid sera against the keratoacanthoma lesion to study the presence or absence of tissue-specific antigens (T.S.A.) in intercellular substance and basement membrane. Finally, the cell-mediated immunity was studied using two in vitro parameters: (a) The estimation of T-lymphocytes through the formation of E-rosettes and (b) the estimation of the leukocyte migration inhibition factor (LIF). Our findings show that specific humoral immune mechanisms are apparently not involved in the spontaneous regression of keratoacanthoma. Cell-mediated immune mechanisms are evidently not responsible for the resolution of the tumor.

Adult↗
PubMed1980

Epidemiology of cutaneous leishmaniasis in Greece.

Cutaneous leishmaniasis is a disease endemic in Greece. Cases collected between the years 1975 and 1979 are analyzed from a clinico-epidemiologic point of view. Prevalence is highest in the Ionian islands and Crete. The disease most commonly affects individuals 10 to 20 years of age. The exposed parts of the body are most commonly involved, particularly the face. The period of highest incidence is mid-winter.

Adolescent↗
PubMed1980

Local hyperhidrosis.

A case of local hyperhidrosis at the ulnar aspect of the left forearm-carpal region with exacerbation, especially during summer, is presented. The sweat function is normal on the remainder of the body. This case is rare, and few similar cases are known in the literature. The possible pathogenetic mechanisms are discussed.

Acetylcholine↗
PubMed1980

Immunohistochemical study on 70 human embryos concerning the epidermal binding of pemphigus and pemphigoid antibodies.

In the present study, the development of pemphigus and bullous pemphigoid antigens was investigated by means of the indirect immunofluorescence technique using sera of pemphigus and bullous pemphigoid patients, respectively, on human foetus skin antigenic substrate. Seventy skin specimens from embryos of 9-38 weeks of gestation were studied. Both pemphigus and bullous pemphigoid antigens were observed for the first time at about 16 weeks of gestation. Pemphigus antigen has a slower rate of evolution. Between 30 and 38 weeks both antigens were detected as strongly positive.

Antigens, Surface↗
PubMed1979

Immunohistochemistry in lichen planus.

An immunohistochemical study has been undertaken on 25 biopsy specmens taken from lichen planus lesions, using antisera against human fibrin, immunoglobulins IgG, IgA, IgM and C3 (B1C/B1A) complement component. The findings of the present research are discussed and evaluated in relation to the problem of the etiopathogenesis of the disease.

Adolescent↗
PubMedDate not supplied

In vivo fluorescence kinetics and photodynamic therapy efficacy of delta-aminolevulinic acid-induced porphyrins in basal cell carcinomas and actinic keratoses; implications for optimization of photodynamic therapy.

Photodynamic therapy (PDT) with topical d-aminolevulinic acid (ALA) has become a therapeutic option of growing interest for superficial non-melanoma precancerous and malignant lesions. After application of ALA, in situ conversion to endogenous porphyrins is accomplished in a gradual manner. Therefore, the determination of fluorescence kinetics and spatial distribution in vivo versus time is a crucial point for the success of ALA-PDT. Seventeen basal cell carcinomas (BCC) and 20 actinic keratoses (AK) were enrolled in this study. In 5 BCC and 4 AK, in vivo fluorescence kinetics were performed over 24 hrs and for the remaining lesions between 2 and 7 hrs after ALA application. In vivo spatial and quantitative detection of the fluorescence intensity versus time showed considerable variations among tumors of the same type, so light irradiation was performed according to patient individualities. Both BCC and AK showed maximal median fluorescence intensity at 4-6.5 hrs post-application. In the present study, a high cure rate was proven after topical ALA-PDT (70.6% in BCC and 85% in AK). The results of fluorescence studies suggest that optimum irradiation time for BCC is approximately 3.5-5 hrs and for AK 5 hrs after ALA application, when relative maximal fluorescence intensity in correlation with fluorescence selectivity on the lesion, is obtained.

Administration, Topical↗
PubMedDate not supplied

Implication of the ras and myc oncoproteins in the pathogenesis of mycosis fungoides.

In the present work, we studied the expression of the c-myc oncoprotein p-62 and the ras oncoprotein p-21 in the dermal cellular infiltrate of paraffin embedded skin specimens, obtained from patients suffering from Mycosis Fungoides and Sezary syndrome. Nineteen specimens from early stage Mycosis Fungoides, nineteen from advanced stage Mycosis Fungoides and four from Sezary syndrome were included in the study. The oncoprotein detection was achieved immunohistochemically, using the mouse monoclonal antibody myc 1-9E10 and the rat monoclonal antibody Y13-259 for p-62 and p-21 respectively. Increased detection of both p-62 and p-21 in atypic lymphoid cells was shown in advanced stages of Mycosis Fungoides (third stage plaques and tumors) as compared to early stages (premycotic erythema, second stage plaques). In advanced stages, however, the percentage of P-62+ atypic cells proved to be higher than that of p-21+ atypic lymphoid cells. The implication of increased p-62 and p-21 oncoprotein expression in the process of lymphomagenesis in cutaneous T-cell lymphomas is discussed.

Antibodies, Monoclonal↗