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Biomedical subjects

A Stelzner

Publications and source records attributed to A Stelzner.

At least 91 records · Page 5Linked to original sources
PubMed1979

[The reverse effect of sexualsteroids on the serum-lysozyme (author's transl)].

75 women received different preparates of contraceptives (Non-Ovlon, Ovosiston, Gravistat, Deposistion--VEB Jenapharm Jena, GDR) for two years. In the last month of the application of the sexual steroids and one month later the lysozyme titer in the sera was tested to determine a reverse or stop-effect. An elevation of the muramidase could be shown in all cases. This findings are interpreted in relation to a non manifest influence of sexualsteroids on the lysozyme.

Adult↗
PubMed1979

[The long-term effect of various hormonal contraceptives on the excretion of sodium, potassium, calcium, magnesium, chloride, phosphorus, uric acid, oxalic acid, citric acid, sulfate and lysozyme in the 24 hour urine].

The influence of a long-term application of 4 different hormonal contraceptives on the excretion of various constituents of the urine was examined. A reverse effect after stoppage of the medication is to be established in none of the other parameters with the exception of calcium.

Calcium↗
PubMed1979

[Immune profil in melanoma patients. III. Behavior of immunglobulins (IgG, IgA, IgM) and of whole complement in serum at the beginning and during BCG-therapy (author's transl)].

The levels of immunoglobulins (IgG, IgA and IgM) and of whole complement were measured in blood serum of 41 patients with malignant melanoma of clinical stage I to III after maximum tumour resection. In all three stages no differences to the normal could be obtained except for IgG demonstrating a rising tendency without statistic significance in patients of stage III. The level of whole complement was likewise normal in patients with the clinical stage I and II. A significant rise of the complement was observed in patients with clinical stage III in the last period of the disease. After one year of nonspecific active immunotherapy with BCG in patients of stages I and II no changes of immunoglobulins could be found. The whole complement showed a rising trend to the upper limit of the normal. The results were discussed in view of the present literature.

Adult↗
PubMed1979

[Further investigations concerning the reaction between haptoglobin and T4-antigen-carrying streptocci (author's transl)].

The median level of haptoglobin types 2-2 and 2-1 was found to be proportional to the agglutination titer of T4 antigen-carrying streptococci (Fig. 1). This relationship need not exist in individual sera since, as seen from Table 1, high agglutination titers may be caused by sera with low levels of haptoglobin. Thus the agglutination reaction might depend not only on the quantity of haptoglobin but also on other factor(s) (at least in individual serum samples). - On the other hand, different agglutination titers did not correlate with the quantity of T4 antigen either. A strain of Strep. pyogenes, type 60, was agglutinated at high titers by sera with a high level of haptoglobin in spite of its low ability to absorb haptoglobin. This was in contrast to a strain of group G (20488) which had a high capacity both to become agglutinated and to absorb haptoglobin (Table 2). Absorption of haptoglobin by affinity chromatography decreased the agglutination titer for T4 streptococci. - The reaction between haptoglobin and T4-streptocci did not fix complement. No differences were found between sera of haptoglobin types 1-1, 2-1, and 2-2 with respect to the amount of C, C3, C4, and C3A, respectively (Tables 3-5).

Agglutination Tests↗
PubMed1979

Cell-mediated immunity to influenza virus antigen and mitogen in guinea pigs: measurement with a semimicro protein synthesis assay.

A rapid and precise method for the assay of cell-mediated immune response basing on protein synthesis stimulation of mitogen-activated guinea pig lymphocytes is modified in a way that enables the study of virus-immunological problems. When used as a micromethod it has the following advantages over conventional methods: short-term cell culture, need of low quantities of cells and rapid preparation of great numbers of samples for radioactivity measurements. In this study we report the results of comparative experiments on measuring lymphocyte stimulation after addition of PHA and stimulation of sensitized lymphocytes following contact with homologous influenza virus antigen in vitro. The most important reaction parameters are as follows: 5-6 . 10(5) spleen lymphocytes/microculture in microtiter plates, use of Eagles's MEM cell culture medium without leucine, supplemented with HEPES buffer and 10% autologous guinea pig serum; optimum lymphocyte stimulation by addition of 0.5 microliter PHA or 0.1-1.0 microgram virus protein/ml; immuno-stimulation by PHA can be measured in vitro already after 6 h and by influenzavirus antigen already after 24 h.

Animals↗
PubMed1978

Influence of counter-irritation by carrageenin on inflammatory reactions induced by prostaglandins, serotonin, histamine, compound 48/80, carrageenin and Freund's complete adjuvant.

The carrageenin edema of rat paw and the primary phase of rat adjuvant arthritis were significantly inhibited by carrageenin whereas the increase of skin capillary permeability induced by prostaglandins (PGs) and further irritants as well as the secondary phase of adjuvant arthritis were mostly not significantly affected. Inhibitory effects after single carrageenin injection do not seem to be mediated by the complement system because total complement activity remained unaffected.

Animals↗
PubMed1978

[Immunological problems of influenza virus infections].

Reference is made to results reported in literature as well as to some of the author's own findings for a summary account of specific immune responses to influenza virus infections. Some introductory comments on the correlations that exist between morphology and function of structural components of the influenza virus as well as on the pathogenesis and its relevance to the immunological responses are followed by a description of the humoral immune response. An assured correlation has been found to exist between the titres of hemagglutination-inhibiting antibodies with responses specific of subtypes and of strains for the protection against homologous influenza virus infection in terms of prevention of outbreak or mitigation of the pathological course. Anti-neuraminidase antibodies have influence upon virus expansion in the organism and in the given population as well as upon the severity of the disease. Particular importance has been attributed to the secretory immunoglobulins. Used in conjunction with interferone, they obviously had an infection-preventing function, wheras the primary role of serum anibodies was a reduction of severity. The studies into cell-mediated immune responses were much less intensive than those conducted into antibody formation. Cell-mediated immune response is thought to emanate from induction of hemagglutinin and is directed against the latter. Sufficient results have not become available as yet on the importance of cellular immune response to protection.

Animals↗
PubMed1978

[Long-term treatment of glomerulonephritis with indomethacin with special regard to quantity and selectivity of proteinuria].

In 53 patients (19 women, 34 men), of them 41 patients with an intracapillary proliferative glomerulonephritis, 9 with membranous proliferative and 3 with a membranous glomerulonephritis under an indometacin therapy examinations concerning proteinuria were carried out, partly with determination of the index of selectivity and representation of the protein clearance. In patients with a moderate proteinuria of less than 3 g a day this treatment achieved a smaller antiproteinurie effect than in patients with a large proteinuria. When a large activity of the inflammatory process was present a therapeutic success was less frequently to be proved. An unequivocal correlation between the histological course of the disease and the change of the proteinuria could not be recognized. After cautious estimation of the findings the selectivity of the proteinuria may be regarded as a certain indicator for the application of indometacin. The protein clearance, however, proved less suitable for the judgment of the treatment performed.

Adolescent↗
PubMed1977

[Experimental allergic chorioretinitis. Appearance and behavior of complement-fixing anti-ROS antibodies in the sera following immunization with retinal antigens (author's transl)].

The authors studied the appearance and behavior of complement-fixing anti-ROS antibodies in the sera of GrCh rabbits following one or two intracutaneous injections of retinal antigens emulsified in Freund's complete adjuvant (KFA). Fourteen different antigenic preparations from heterologous, homologous, and autologous retinal components incorporated in KFA were tested and compared. Apart from the supernatant of a centrifuged homogenized preparation of homologous retina, all immunogens generally induced anti-ROS antibodies. The antibody titers (reciprocal values) reached a maximum 4 to 8 weeks post injection, at only low levels. The changes in antibody concentrations showed some relationship to the quality and dose of the immunogens. Anamnestic effects following reinjection reached maximum values within as little as 5 to 14 days. Animals not developing chorioretinitic lesions showed higher antibody titers more frequently than rabbits with clinically confirmed disease. Provided there is sufficient relevance of the humoral reactions observed, the complement-fixing antibodies we found in our experiments are more likely to have protective than pathogenic functions.

Animals↗
PubMed1977

[Studies on calf salmonellosis. 4. Oral and parenteral immunization with live (Smd) and killed antigens].

Reported are results obtained from studies into oral and parenteral immunisation of calf. The approaches had included the use of live (Smd) or dead antigen from Salmonella (S.) dublin and a combination of the two immunisation methods. Live antigen (Smd) was superior to thermally activated dead antigen, when the oral route was used to prevent S.-dublin injection of calves. The above findings were supported by results from analogous studies in which S. typhimurium and S. dublin or live antigen (Smd) or dead antigen, made of the two, had been applied to mice. (One single subcutaneous) parenteral administration did hardly reveal any difference in favour of live vaccine (Smd). Parenteral administration of live or dead antigen proved to be less effective than repeated oral immunisation, particularly when live vaccine (Smd) was used. Immunity not less than up to six months of age against S. dublin wild strain infection can be provided for young calves by oral immunisation, with Smd vaccine (5. 1010 to 1. 1011 live germs/d) being given on ten consecutive days. Calves orally immunised with live antigen (ten repetitive applications of Smd mutants) are likely to develop an antibody titre (H-agglutinins) against S. dublin. Parenteral boostering,using live antigen, has been accompanied by sensitisation due to oral live antigen administration as well as by dose dependence, as was seen from the bactericidal values. Sensitisation was established from orally immunised calves up to three months old (typical booster reaction). Some of it was attributabale to confrontation with wild strains of Salmonella. The H-agglutinin titres of animals aged threemonths in a calf herd with salmonelloses in which all animals had been orally Smd-immunised were close to those recorded from calves in stocks with no salmonellosis occurrence. Under the conditions of oral immunisation, there had obviously been no action of the wild strain which might have triggered intensive antibody formation.

Administration, Oral↗
PubMed1976

[Studies with the macrophage migration inhibition test in patients with rheumatoid arthritis].

The authors intended to investigate whether lymphokinins can be verified in rheumatoid arthritis, and if so - to what extent. They used the macrophage-migrations-inhibition test (MMIT). The validity of the experimental model was therefore tested in patients with tuberculosis. In 97 examinations with 76 exactly defined R.A.-patients no significant migrationsinhibitions were present, apart from single findings. Various synovialis extracts and R.A.-synovia were used. The experimental conditions were varied up to a maximum factor of 8. The results obtained were discussed from the point of view of methodical accuracy, unsatisfactory preparation of antigens and possible double inhibition effects.

Arthritis, Rheumatoid↗