'The longevity of occlusal amalgam restorations in first permanent molars of child patients'.
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Child↗
Biomedical subjects
A Stanley
Publications and source records attributed to A Stanley.
Noninvasive diagnosis of right cervical aortic arch.
A two-year old asymptomatic boy presented with a systolic right supraclavicular thrill. Two-D echography revealed a pulsating loop of tubular echo-free space at this site. Barium esophagogram showed a large curved retroesophageal impression. A noninvasive diagnosis of right cervical aortic arch with left descending aorta can be made on the basis of this combination of findings.
Aorta, Thoracic↗
Migraine treated by inhalation sedation using nitrous oxide and oxygen.
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Adult↗
Mitral valve prosthesis for correction of mitral regurgitation in a dog.
Mitral regurgiation with atrial fibrillation in a 3-year-old St. Bernard dog was corrected by digitalization, placement of a mitral valve prosthesis, and direct current cardioversion. The atrial fibrillation eventually returned and became refractory to cardioversion.
Animals↗
The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis--preliminary findings.
Proteomics-based studies offer a powerful complementary approach to DNA/RNA-based investigations and are now being applied to investigate aspects of many diseases including cancer. However, the heterogeneous nature of tissue samples often makes interpretation difficult. We have undertaken a study into the potential use of a novel laser capture microdissection (LCM) system to isolate cells of interest for subsequent proteomic analysis. Retrieval of selected cells is achieved by activation of a transfer film placed in contact with a tissue section, by a laser beam (30 or 60 microm diameter) which is focused on a selected area of tissue using an inverted microscope. The precise area of film targeted by the laser bonds to the tissue beneath it and these cells are then lifted free of surrounding tissue. Although the technique has been shown to be readily compatible with subsequent analysis of nucleic acids, little information is yet available regarding the application of protein-based analyses to the captured tissue. We report here preliminary data regarding the potential use of the LCM system in combination with two-dimensional electrophoresis to examine protein profiles of selected tissue areas. Electrophoretic profiles of proteins from normal and malignant renal tissue samples showed little change following LCM, nine selected proteins showed identical mass spectrometric sequencing profiles, and two selected proteins retained antigenicity. Dissection of epithelial tissue from a sample of normal human cervix resulted in enrichment of some proteins compared with analysis of the whole tissue. LCM will be a valuable adjunct to proteomic studies although further detailed validation is necessary.
Amino Acid Sequence↗
Prevalence of neurosyphilis at Government Rajaji Hospital, Madurai, India.
Forty patients (34 males and 6 females) with neurological complaints/manifestations and with a past history of multiple sexual partners attending the Government Rajaji Hospital, Madurai, India between April 1992 and October 1992 were investigated for neurosyphilis. Metabolic disorders, hypertension, ischaemic heart disease, arrhythmias and trauma were excluded. Seven males (17.5%) were found to have neurosyphilis. The youngest was 26 years old and the oldest was 47. All were married and of low socioeconomic background. Meningovascular syphilis was the predominant presentation (6:1). Associated cardiovascular involvement was noticed in one of the cases. There was no associated HIV infection in these cases. The incidence is higher than previous reports from this centre.
Adult↗