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Biomedical subjects

A Shiba

Publications and source records attributed to A Shiba.

At least 55 records · Page 3Linked to original sources
PubMed1984

An ultramicro-assay for proteins in biological fluids other than blood using a combination of agarose gel isoelectric focusing and silver staining.

Agarose gel isoelectric focusing and silver staining, is a suitable method for clearly separating 20-30 protein bands in various body fluids other than blood, such as urine, cerebrospinal fluid, tear, sweat, saliva and gingival fluid. A protein concentration of 80 mg/l is sufficient for detection, and a range of 200-600 mg/l is optimum to obtain reliable results. Thus, no preparative concentration or desalting of samples is required, though dilution to the optimum protein level may be needed for some fluid samples. This technique is expected to provide diagnostically useful information.

Body Fluids↗
PubMed1983

Analysis of human palatine salivary proteins by isoelectric focusing in agarose.

An electrophoretic method capable of analysing a small volume (5-10 microliter) of human palatine saliva without prior concentration was developed by combining the high resolution of isoelectric focusing in agarose with a highly sensitive silver protein staining method. Human palatine saliva exhibited 23 protein bands, those at pI 4.3-4.4, 4.7, 5.3 and 5.8 being major bands.

Humans↗