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Biomedical subjects

A Shiba

Publications and source records attributed to A Shiba.

At least 37 records · Page 2Linked to original sources

[Prosthetic rehabilitation with the removable partial dentures using telescopic crowns after removal of unsuccessful dental implants].

It is well-known that unsuccessful dental implants sometimes give rise to pronounced inflammatory bone resorption of the upper and lower jaw, and that in such cases it is practically much difficult to attain adequate prosthodontic rehabilitation after removal of the implants. On this study it is completely reported that two patients suffering from implant-induced osteomyelitis could be finely compensated for with the removable partial dentures using telescopic crowns after removal of the implants, and then the results are shortly discussed from the view of pathology, oral surgery and prosthodontics. In the first case a 52-year-old woman had been suffering from osteomyelitis of the right mandible due to the both bladevent and endodontic-endosseous implant, and in another case a 43-year-old woman had been suffering from osteomyelitis of the right maxilla due to the endosseous subperiosteal implant, and they had borne insufficient mastication on the affected side of the jaws. Several months after removal of the unsuccessful dental implants, they were rehabilitated with the removable partial dentures using telescopic crowns to recover and maintain good functions such as occlusion, mastication, cosmetics and speech, and oral and general health.

Adult↗

Re-evaluation of normal human urinary proteins fractionated by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis.

Proteins in normal human urine were clearly fractionated into 26 bands with molecular weights from 14,000 to 230,000 by means of one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) coupled with silver staining. The main band contained uromucoid, and the second main band had albumin. However, when urine samples from healthy persons were electrophoresed in the absence of SDS using polyacrylamide gel or agarose gel, or a cellulose acetate membrane, albumin but not uromucoid, frequently formed the main protein band. It is suggested that this is due to the complexing of uromucoid subunits to form a large molecule which cannot penetrate into the gel. In order to correctly fractionate all the proteins contained in normal human urine, it was concluded that it was best to treat a urine sample with SDS with pre-condensation, fractionate it by SDS-PAGE and stain fractionated proteins by a highly sensitive method such as silver staining.

Adult↗

Analysis of salivary proteins by thin layer sodium dodecylsulphate polyacrylamide gel electrophoresis.

Samples of the whole, parotid, submandibular and palatine salivas and serum, all collected from the same human subject, were developed by simultaneous electrophoresis on a single SDS thin layer gel plate, and simple proteins, glycoproteins and sialoglycoproteins in the samples were isolated and analysed. Simultaneous electrophoresis of the salivas and serum showed the presence of thirty-four protein bands in serum, twenty-two bands in whole saliva, sixteen bands in parotid saliva, nineteen bands in submandibular saliva and ten bands in palatine saliva. Glycoprotein bands in the isolating gel zone presented five bands in whole saliva, four bands in submandibular saliva, three bands in parotid saliva and no band in palatine saliva. Palatine saliva, however, showed a single clear band in the concentrating gel zone. Palatine saliva showed only one sialoglycoprotein band in the concentrating gel zone.

Adult↗