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Biomedical subjects

A Schulze

Publications and source records attributed to A Schulze.

At least 163 records · Page 9Linked to original sources
PubMed1985

[Clinical and computed tomographic observations on newborn infants with generalized brain edema due to perinatal asphyxia].

We observed a sudden respiratory arrest in four term newborn infants after a clinically symptom-free period. There were no cardiac, pulmonary or metabolic changes responsible for these events. Signs of cerebral dysfunction existed (muscular hypotonia, jitterness, seizures). Cranial computerized tomographic scans were performed during the period of respirator treatment within the first week of life. The density of the brain structures was significantly decreased in all cases, three of the patients showed a complete compression of the lateral ventricles. These findings indicated severe brain swelling as a consequence of cerebral hypoxia. There was a history of umbilical cord occlusion in two cases. In the remaining patients we must assume an undetected hypoxic-ischemic episode prior to the onset of labor. We used hyperventilation, corticosteroids, phenobarbital, diuretics and fluid restriction for therapy. Later on the babies received special physiotherapy. Control CT-scans were performed during the fourth or fifth week of life. The findings were normal in one patient. Signs of mild focal brain atrophy developed in two babies. A more severe cortical atrophic lesion of both temporal lobes was found in one patient. He suffered from a slight cerebral palsy. No neurodevelopmental handicaps could be found in all the other patients on long term follow-up. The EEG examination was performed between the fifth and seventh month of life. No pathologic changes were observed. We conclude that severe generalized brain edema in the newborn is not necessarily followed by extensive brain damage. We think it important to develop more sensitive methods for detecting a hypoxic ischemic crisis preceding the birth.

Asphyxia Neonatorum↗
PubMed1985

[Initial experiences in the detection of psychodynamic conflicts].

A few extracts from an "anamnesis sheet for neurotics" are presented that has been in use at the psychotherapy department of the neurological clinic of the Charité since 1981. The author reports on the experience that has been gained in the use of this sheet.

Conflict, Psychological↗
PubMed1984

Transmission of the marker X syndrome trait by unaffected males: conclusions from studies of large families.

It is well established that apparently unaffected males can be transmitters of the marker X syndrome trait. Cytogenetic and clinical investigations of these male transmitters are only rarely reported for most of these male transmitters are dead by the time the syndrome is diagnosed in their families. We report on cytogenetic and clinical investigations of two unaffected male carriers of the disorder from two large families. Pedigree analysis of these families revealed six other cases of possible male transmission of the marker X syndrome trait. Mental impairment was not reported from the siblings of these unaffected male carriers and could not be observed in their daughters. The mode of transmission of the disorder cannot be fully explained by X-linked inheritance. The phenomenon of unaffected males transmitting the disorder could be due to an autosomal suppressor systeme. Our findings indicate that male transmission may be important for the frequency of the disorder.

Adolescent↗
PubMed1984

Response to gravity by Zea mays seedlings. I. Time course of the response.

Gravistimulation induces an asymmetric distribution of free indole-3-acetic acid (IAA) in the cortex-epidermis of the Zea mays L. cv 'Stowells Evergreen' mesocotyl within 15 minutes, the shortest time tested. IAA was measured by an isotope dilution method as the pentaflurobenzyl ester. The per cent IAA in the lower half of the mescotyl cortex was 56 to 57% at 15, 30, and 90 minutes after stimulus initiation. Curvature is detectable in the mescotyl within 3 minutes after beginning gravitropic stimulation. The rate of curvature of the mesocotyl increases during the first 60 minutes to maximum of about 30 degrees per hour. Thus, the growth asymmetry continues to increase for 45 minutes after hormone asymmetry is established. Free IAA occurs predominantly in the stele of the mesocotyl whereas esterified IAA is mainly in the mesocotyl cortex-epidermis. This compartmentation may permit determining in which tissue the hormone asymmetry arises. Current data suggest the asymmetry originated in the stele.

Cotyledon↗
PubMed1984

Gravity-induced asymmetric distribution of a plant growth hormone.

H.E. Dolk demonstrated that gravistimulation induced an asymmetric distribution of auxin in a horizontally-placed shoot. We wish to determine where, and how that asymmetry arises and have demonstrated that the endogenous auxin, indole-3-acetic acid, becomes asymmetrically distributed in the cortical cells of the Zea mays mesocotyl during 3 min of geo-stimulation. Further, indole-3-acetic acid derived by hydrolysis of an applied transport form of the hormone, indole-3-acetyl-myo-inositol, becomes asymmetrically distributed within 15 min of geo-stimulus time. From these and prior data we have developed a working theory that the gravitational stimulus induces a selective leakage, or secretion, of the hormone from the vascular tissue to the cortical cells of the mesocotyl.

Biological Transport↗
PubMed1984

[Borderline and narcissistic personality disorder--fashionable concepts or approaches to a new etiopathogenic and therapeutic understanding?].

On the basis of the experience in own clinical practice with severe disturbance of personality and literature of this time about neurosis conceptional reflections are discussed the terms "borderline" and "narcissism". It is pointed out, that the described terms stimulate to psychological understanding of classes of abnormal personalities and to psychotherapy of these patients.

Borderline Personality Disorder↗
PubMed1983

Gravitational effects on plant growth hormone concentration.

Numerous studies, particularly those of H. Dolk in the 1930's, established by means of bio-assay, that more growth hormone diffused from the lower, than from the upper side of a gravity-stimulated plant shoot. Now, using an isotope dilution assay, with 4,5,6,7 tetradeutero indole-3-acetic acid as internal standard, and selected ion monitoring-gas chromatography-mass spectrometry as the method of determination, we have confirmed Dolk's finding and established that the asymmetrically distributed hormone is, in fact, indole-3-acetic acid (IAA). This is the first physico-chemical demonstration that there is more free IAA on the lower sides of a geo-stimulated plant shoot. We have also shown that free IAA occurs primarily in the conductive vascular tissues of the shoot, whereas IAA esters predominate in the growing cortical cells. Now, using an especially sensitive gas chromatographic isotope dilution assay we have found that the hormone asymmetry also occurs in the non-vascular tissue. Currently, efforts are directed to developing isotope dilution assays, with picogram sensitivity, to determine how this asymmetry of IAA distribution is attained so as to better understand how the plant perceives the geo-stimulus.

Avena↗
PubMed1983

Effect of Deseeding on the Indole-3-acetic Acid Content of Shoots and Roots of Zea mays Seedlings.

We wished to determine the effect of endosperm removal on the amounts of free and esterified indole-3-acetic acid (IAA) in young Zea mays seedlings. The increases of IAA derived from endosperm and from biosynthesis, but without correction for catabolic losses, were 0.9 picomole of free IAA per shoot per hour, and 1.1 picomoles per shoot per hour of ester IAA. After deseeding, free IAA in the shoot declines by 40% following kernel removal and total (free + ester) IAA declines at a rate of about 1 picomole per shoot per hour. A slight, but insignificant increase of ester IAA occurs following endosperm removal. In the primary roots, the decreases of free IAA and total (free + ester) IAA are accelerated by seed removal. Thus, the endosperm appears to be a major source of IAA for the shoot and root.

Journal Article↗
PubMed1982

Distribution of Free and Ester Indole-3-Acetic Acid in the Cortex and Stele of the Zea mays Mesocotyl.

The distribution of free and ester-linked indole-3-acetic acid (IAA) in the vascular stele and cortex-epidermis of the Zea mays mesocotyl was measured by gas chromatography-selected ion monitoring-mass spectrometry and by radioimmunoassay with good agreement between the two assay methods. On a per plant basis, 72% of the free IAA was found in the stele and 28% was in the cortex, whereas 80% of the ester IAA was in the cortex with 20% localized in the stele. On a fresh weight basis, the concentration of free IAA was 15 to 28 times higher in the stele than in the cortex, whereas the concentration of ester IAA was similar in the two tissues. The concentration of free IAA in the apical portion of the cortex was 3 times higher than in the basal portion, and this distribution correlated with the relative growth rates of the apical and basal portions of the mesocotyl. No changes in the longitudinal distribution of ester IAA were found in either the cortex or stele.

Journal Article↗
PubMed1982

[Selected results from a study on the life style of elderly females in a retirement home].

The article deals with the living conditions of 26 women in an old people's home before and after they had moved into the home. The motives for moving into a home and some problems of these people's activities and social contacts in the home are discussed. Then some aspects of the biographies are assessed. The results of the cases are not representative, they do not claim to be comprehensive, they only show tendencies.

Activities of Daily Living↗
PubMed1981

Validation of a radioimmunoassay for indole-3-acetic Acid using gas chromatography-selected ion monitoring-mass spectrometry.

A radioimmunoassay for indole-3-acetic acid (IAA) has been validated by comparison with a physico-chemical assay utilizing gas chromatography-selected ion monitoring-mass spectrometry and 4,5,6,7-tetradeutero-indole-3-acetic acid as an internal standard. The radioimmunoassay provided a reliable estimate of the free IAA content of etiolated corn shoots. However, base hydrolysis of extracts for determination of ester IAA released substances which interfered with the radioimmunoassay. Interference was detected by internal controls and by lack of agreement with the mass spectral method. Interfering compounds could be removed from extracts by chromatography on diethylaminoethyl- and hydroxypropylated (lipophilic) Sephadex G-25. Following such purification the radioimmunoassay estimate of the total (free + ester) IAA content of etiolated corn shoots agreed with the mass spectral method within 2% on the average.

Journal Article↗
PubMed1980

Synthesis of 4,5,6,7 and 2,4,5,6,7 Deuterium-labeled Indole-3-Acetic Acid for Use in Mass Spectrometric Assays.

Syntheses are described for tetra and pentadeutero indole-3-acetic acid (IAA) labeled in positions 4, 5, 6, 7 or 2, 4, 5, 6, 7 of the indole moiety. Polydeuterated IAA is proposed as an internal standard for gas chromatographic-mass spectrometric analysis of IAA by selected ion monitoring. Nanogram amounts of IAA may be assayed by monitoring the base peak of IAA at m/z = 130 (134 for d(4)-IAA) and the molecular ion of the methyl ester of IAA at 189 (193 for d(4)-IAA). Deuterium in positions 4, 5, 6, and 7 and, to only a slightly lesser extent, that in position 2 of IAA is retained during alkali treatment, thus permitting use of these compounds as internal standards for assay of IAA released by alkaline hydrolysis of ester and amide conjugates. The use of polydeutero internal standards separates the standards from the "isotope cluster" caused by the normal abundance of heavy isotopes and also permits use of reduced mass resolution, thus leading to a 10-fold increase in sensitivity.Tetradeutero IAA was used as an internal standard for determining free plus ester IAA in alkaline hydrolysates of Zea mays, and showed exact agreement between estimates based on the molecular ion of the methyl ester and those based upon base peak. Application of the method to measuring free IAA in the upper and lower halves of geotropically stimulated Zea shoots showed 61 +/- 4% of the free IAA to be on the lower side.

Journal Article↗
PubMed1980

[Immunohistochemical detection of antigammaglobulin factors in the serum (author's transl)].

Agglutination procedures for the detection of rheuma factors are inadequate concerning IgG and IgA factors and sensitivity to IgM factors, too, is generally limited. Both these disadvantages are to a great extent overcome when rheuma factors are fluorescence-marked with anti-human-gammaglobulin respectively with monospecific antiserums and when reaction steps according to Svartz and Schlossmann are applied to a microscope slide test. This test and the results of investigations of 101 cases (different joint diseases and healthy blood--donors) are described in this paper.

Agglutination Tests↗