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Biomedical subjects

A Sano

Publications and source records attributed to A Sano.

At least 271 records · Page 15Linked to original sources
PubMed1975

Common atrium associated with anomalous high insertion of the inferior vena cava.

The case is reported of a 38-year-old housewife with a common atrium associated with anomalous high insertion of the inferior vena cava, absence of the coronary sinus, and drainage of a left hepatic vein into the left side of common atrium. Surgical repair was performed successfully. The procedure was closure of a nearly total atrial septal defect with a Dacron patch. Embroyologically, the development failure of the sinu-atrial fold appears to be the basic causal factor.

Adult↗
PubMedDate not supplied

Fluorescence quenching properties and chemiluminescence responses of alpha-ketothiols derivatized with o-phthalaldehyde and primary amino compounds.

Fluorescence and chemiluminescence responses of thiols derivatized with o-phthalaldehyde (OPA) and primary amines have been studied. Among thiols tested, those having an alpha-ketothiol structure [R-CH(SH)-CO-] were found to give extremely low fluorescence intensities when derivatized with OPA and L-valine. The relative fluorescence intensities of alpha-ketothiols, such as mercaptoacetic acid (MAA), 2-mercaptopropionic acid (2-MPA), mercaptosuccinic acid (MSA) and N-(2-mercaptopropionyl)glycine (MPG), were only below 3% of that of N-acetyl-L-cysteine (NAC), having no alpha-ketothiol structure. The addition of detergents such as sodium dodecylsulphate had no effect on the fluorescence and an aprotic solvent (dimethylsulphoxide) showed little effect, whereas these thiols chemiluminesced intensely when derivatized with OPA and N-(4-aminobutyl)-N-ethylisoluminol as a primary amine and successively detected by high-performance liquid chromatography (HPLC) with postcolumn chemiluminescence reactions using hydrogen peroxide and haematin. The relative chemiluminescence intensities of MSA, 2-MPA and MPG to NAC were 41.0%, 27.8% and 117%, respectively. MPG was estimated to be detectable at a concentration level of at least 10 fmol per injection. In conclusion, the weak fluorescence of the alpha-ketothiols derivatized with OPA and primary amines is probably due to the low fluorescence quantum yields of the derivatives, and HPLC with a chemiluminescence detection system is useful for the sensitive detection of thiols.

Amines↗
PubMedDate not supplied

Comparison between human and armadillo Paracoccidioides brasiliensis isolates by random amplified polymorphic DNA analysis.

Sixty-three Paracoccidioides brasiliensis isolates obtained from three nine-banded armadillos (Dasypus novemcinctus), one Amazonian armadillo's and 19 clinical isolates were compared by random amplified polymorphic DNA analysis with the primer OPG-19. The isolates were divided into three major clusters, I, II and III. Coincidences between human and armadillo isolates were observed in clusters I and II. Cluster III consisted only of armadillos' isolates. The results suggested that (I) humans may acquire P. brasiliensis infection by contact with armadillo's environment, (II) there may be P. brasiliensis genotypes peculiar to the animal, and (III) individual armadillos may be infected with P. brasiliensis cells with different genotypes.

Animals↗
PubMedDate not supplied

Pathogenicities and GP43kDa gene of three Paracoccidioides brasiliensis isolates originated from a nine-banded armadillo (Dasypus novemcinctus).

We studied three different isolates of Paracoccidioides brasiliensis obtained from the mesenteric lymph node (D3LY1), the spleen (D3S1) and the liver (D3LIV1) of the same armadillo (Dasypus novemcinctus). Pulmonal inflammatory area was evaluated by intravenous inoculation of 10(6) yeast cells of each isolates in young, male, ddY mice. Moreover, the partial sequence of GP43kDa gene of P. brasiliensis was analyzed. The lung inflammatory area was greater in animals inoculated with isolate D3S1. The partial sequence of GP43kDa gene indicated that isolate D3S1 is different from isolates D3LY1 and D3LIV1. This study suggested that the same armadillo might be susceptible to multiple P. brasiliensis isolates simultaneously.

Animals↗
PubMedDate not supplied

Upregulated surface expression of intracellularly sequestered Igepsilon receptors (FcepsilonRII/CD23) following activation in human peripheral blood eosinophils.

We investigated the regulation, secretion, and surface expression of the low-affinity FcepsilonRII receptor (CD23) in eosinophils isolated from human blood using multiple monoclonal antibodies (mAbs) directed at different epitopes of human CD23. Substantial surface expression of CD23 was not demonstrated in the resting state. Mean fluorescence intensity (MFI) measured by flow cytometry was 7. 1 +/- 0.8 for 9P25 mAb (p = NS) and 15.7 +/- 3.8 for BU38 mAb (p <. 04) versus 5.3 +/- 1.0 for IgG1 isotype control Ab. By contrast, MFI using BU38 mAb was 154 +/- 18 for JY-B lymphocytes (p <.0001 versus eosinophils). Despite weak surface expression, eosinophil permeabilization demonstrated substantial intracellular expression of CD23; MFI was 33.6 +/- 5.2 for 9P25 mAb versus 4.4 +/- 0.43 for IgG control (p <.001). Western blot analysis using both positive and negative controls demonstrated immunological identity with CD23 on JY-B lymphocytes. Activation of eosinophils caused rapid translocation of CD23 to the surface membrane (160 +/- 33 MFI; p <. 005), which was maximal within 30 sec. Secretory CD23 was detected within the perfusate also at 30 sec and was fully reinternalized at 10 min. This is the first demonstration of the presence of intracellular CD23 in human eosinophils. Our data indicate that eosinophils rarely express CD23 on their surface but are capable of transient high-level expression and secretion with rapid reuptake of intracellular stores of CD23.

Cells, Cultured↗