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Biomedical subjects

A Salmi

Publications and source records attributed to A Salmi.

At least 73 records · Page 4Linked to original sources
PubMed1988

Small hepatocellular carcinoma: percutaneous alcohol injection--results in 23 patients.

Twenty-three patients with hepatocellular carcinoma less than 4.5 cm in diameter (32 lesions) were treated with a percutaneous injection of ethyl alcohol performed with ultrasound (US) guidance. No complications or sequelae occurred after 271 treatments. All lesions were smaller after 6-27 months follow-up. No evidence of hepatocellular carcinoma was present at fine-needle biopsy or contrast material-enhanced computed tomography (CT). No viable tumor cells were detected in four patients who subsequently underwent surgery. The 1-year survival rate of 12 patients was 91.7%. From these data, it can be concluded that percutaneous alcohol injection (PAI) is an appropriate first-line treatment for patients with small hepatocellular carcinoma lesions who are poor surgical risks. Given the risk of new nodular lesions occurring on resected livers and the risk of perioperative mortality, PAI might even be considered an alternative to surgery.

Aged↗
PubMed1988

Measles virus infection of unstimulated blood mononuclear cells in vitro: antigen expression and virus production preferentially in monocytes.

Synthesis of measles virus antigens occurred only in a small percentage of peripheral blood mononuclear cells infected in vitro with measles virus without mitogenic stimulation. The infection cycle was restricted as only low amounts of infectious virus were released but all the major structural viral proteins were present as shown by immunofluorescence with monoclonal antibodies. Cells with viral antigen synthesis were characterized by double labelling and by infecting selectively depleted subpopulations. In 3-day cultures, up to 80% of the cells with measles virus antigen were shown to be monocytes by specific staining with anti-MMA and anti-Leu M3 monoclonal antibodies and up to 40% of the monocytes were infected. Less than 10% of the cells expressing virus antigens carried the lymphocyte marker OKT3, the majority of these cells belonging to the Leu3a (helper) population. Anti-alpha-interferon treatment increased the number of measles-positive cells and the release of infectious virus in preparations enriched for monocytes, but had no significant effect on infection of lymphocytes.

Antibodies, Monoclonal↗
PubMed1987

Is it possible to recognize portal hypertension with continuous-wave Doppler ultrasonography?

Continuous-wave (CW) Doppler ultrasonography is a well-known technique in the assessment of peripheral and central circulation. The purpose of this study was to find out whether the technique can be used to demonstrate portal hypertension (PH) from liver disease through the evaluation of flow reversal in the veins of the round ligament. One hundred eight patients (50 controls and 58 with PH) underwent abdominal sonography, endoscopic examination, and CW Doppler flowmetry of the umbilical region. In 53 of 58 patients with PH, hepatofugal venous flow was found in the round ligament; this sign was absent in all of the controls. There was remarkable agreement between the results of CW Doppler examination and those of endoscopy and abdominal sonography: its sensitivity was about 91%, and we encountered no false positives. The Doppler ultrasound method is specific for diagnosing PH, simple to perform, and repeatable.

Female↗
PubMed1987

NK activity and NK-like non-specific cytolysis after PPD, rubella and measles antigen stimulation in multiple sclerosis.

Blood samples were collected from 14 pairs of multiple sclerosis (MS) patients and from age- and sex-matched healthy controls. The cytotoxic activity of peripheral blood mononuclear cells was tested against the K562 cell line on the day of collection and again after 3 days in vitro culture with medium or with purified protein derivative of tuberculin (PPD), inactivated rubella virus or inactivated measles virus antigen. Lymphocytes from MS patients had a lower spontaneous cytotoxic activity compared to the controls both on the day of collection (P less than 0.025) and after 3 days in culture with medium alone (P less than 0.025). The activity decreased during in vitro culture in both groups, but the decrease was greater among MS patients (P less than 0.05). In cultures with antigens, a strong increase of NK-like cell-mediated cytolysis (NK-like CMC) was noted especially in cultures stimulated with PPD. There were no significant differences in the increase of the activity among MS patients and control subjects. A significant correlation between the increase of NK-like CMC and the lymphocyte blast transformation response induced by each antigen was found.

Adult↗
PubMed1987

Measurement of polypeptide- and antigenic site-specific antibodies to measles virus using a competitive enzyme immunoassay.

A competitive enzyme immunoassay (EIA) for measuring polypeptide- and antigenic site-specific antibodies against measles virus (MV) was developed. Three monoclonal antibodies (MAbs) against the fusion (F) and one MAb against the hemagglutinin (H) protein of MV were labelled with biotin and used in biotin-streptavidin EIA system. The inhibition of the binding of the labelled MAbs by the bound serum samples was measured in EIA and the titers were defined by reading at the level of 25% inhibition. The titers increased from acute to early convalescent measles sera in all the tests and antibody levels to the F protein increased earlier than antibodies to the H protein. The ratio of antibodies against 2 different antigenic sites on the F protein was significantly higher in subacute sclerosing panencephalitis patients than in normal blood donors. Similarly, SJL mice immunized with MV antigen had higher ratios of the corresponding antibodies than Balb/c mice. The results indicate that antibody response to different polypeptides and antigenic sites on the same polypeptide can be studied with the developed method and that the responses against different antigenic sites are independent.

Animals↗
PubMed1987

Characterization of in vivo activated lymphocytes found in the peripheral blood of patients undergoing cardiac operation.

We studied in vivo lymphocyte activation in patients who underwent cardiac operation. A large number of deoxyribonucleic acid-synthesizing cells characterized by 3H-thymidine uptake and morphologically atypical lymphocytes were found after operation in the peripheral blood samples of patients, the peak level occurring on the sixth or seventh postoperative day. Most of the deoxyribonucleic acid-synthesizing cells were in the B cell fraction. The number of antibody secreting cells in the patients' peripheral blood samples was found increased from the normal level after operation. Immunoglobulin A-secreting cells were the most elevated fraction. The percentage of helper (OKT4) and suppressor (OKT8) cells in the postoperative samples was not significantly different from that in the preoperative samples. Only a few were Ia antigen positive cells. Co-culture of patients' B cells with their own T cells and with the T cells taken from two healthy controls revealed no signs of any helper or suppressor effect on these antibody-secreting cells. The results show that after cardiac operation the peripheral blood of patients contains an increased number of immunoglobulin secreting cells. The regulatory T-lymphocytes seem not to have any effect in vitro on these in vivo activated cells.

Adult↗
PubMed1987

Immunological findings during an acute relapse of subacute sclerosing panencephalitis in a patient with an unusually prolonged disease course.

A 171/2-year-old male subacute sclerosing panencephalitis patient underwent a disease relapse after an 8 year remission. A total of 14 paired peripheral blood/cerebrospinal fluid specimens were obtained during this relapse. These specimens were tested for 13 parameters of non-specific or measles virus-specific cell-mediated and humoral immunity. There was a rapid and significant fluctuation in all of these tests. Total loss of measles-specific T cells response was found during the relapse. The response re-appeared and measles-specific antibodies increased significantly later on. The results suggest that measles virus infection was reactivated in this patient and that the observed immunological changes could at least partially be explained by this.

Adolescent↗
PubMed1986

[Serum cholylglycine in the evaluation of impaired hepatic function].

The biliary serum salts cholylglycine and sulfolithocholylglycine were assessed in 88 chronic liver disease patients subjected to laparoscopy and biopsy. The assay was conducted before and after meals in order to assess the diagnostic value of the test together with the common liver function parameters. The greatest diagnostic sensitivity was provided by gamma glutamyl transpeptidase and bromosulfophtalein K1 clearance. In particular fasting glycocholic acid was as predictively reliable as BSF-K1. Cholylglycine levels also correlated with the prognosis of the liver disease.

Clinical Enzyme Tests↗
PubMed1986

Circulating immune complexes in patients with subacute sclerosing panencephalitis.

Levels of immune complexes (ICs) in serum and cerebrospinal fluid (CSF) specimens from subacute sclerosing panencephalitis (SSPE) patients were measured using a solid-phase C1q radioimmunoassay. Single or serial serum specimens were available from 19 patients, while serial CSF specimens were available from two patients. ICs isolated from one CSF specimen by C1q immobilized to Affi-Gel were analyzed for measles virus antigens by binding of measles virus-specific antisera and by polyacrylamide gel electrophoresis followed by Western blotting. Of 78 serum specimens analyzed, 36 (46%) were positive for ICs. When the 8 patients with 3 or more serum specimens were analyzed, 6 had fluctuating levels of ICs. Two of 5 CSF specimens obtained from a patient during acute disease onset were IC-positive, while a second patient exhibited a rapid increase and decrease of IC levels in 14 CSF specimens obtained during an acute disease exacerbation. Composition analysis of ICs isolated from one of the CSF specimens revealed the presence of antigens corresponding in size to measles virus polymerase, nucleoprotein, and possibly, hemagglutinin polypeptides. These results show that SSPE patients frequently have ICs, that IC levels fluctuate in both serum and CSF, and that the ICs are at least partially composed of measles virus antigens.

Antigen-Antibody Complex↗