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Biomedical subjects

A Robertson

Publications and source records attributed to A Robertson.

At least 361 records · Page 20Linked to original sources
PubMedDate not supplied

Functional imaging of brain responses to repetitive sensory stimulation: sources estimated from EEG and SPECT.

This study examined the effect of modality of stimulation on two measures of cerebral function: (a) the scalp distribution of sensory evoked potentials and (b) the cerebral distribution of radiolabelled HMPAO. Steady-state stimulation in the auditory, somatosensory or visual modality was presented to six subjects. Scalp potentials were measured from a distribution of electrodes, and the radiopharmaceutical was injected through an indwelling intravenous catheter midway through the stimulation/recording session. Equivalent dipole sources estimated from the spatial distribution of the scalp potentials were found to be consistent with regions of high HMPAO uptake as imaged by Single Photon Emission Computed Tomography (SPECT).

Acoustic Stimulation↗
PubMedDate not supplied

The magnetoencephalographic localisation of source-systems in the brain: early and late components of event related potentials.

The application of magnetoencephalography (MEG) to the analysis of sources in the brain responsible for early and late components of evoked potentials is discussed. Representative data are presented and discussed which demonstrate localisation of sources assuming single equivalent dipoles. Distributed systems as sources for some steady-state responses are discussed in relation to the broader issue of the usefulness of equivalent single dipole models. These issues are related to the use of MEG for the analysis of source systems influenced by alcohol and other drugs.

Animals↗
PubMedDate not supplied

HLA-G1 protein expression is not essential for fetal survival.

HLA-G is a nonclassical, class I HLA gene that is primarily expressed by fetal cells at the maternal-fetal interface and is thought to play a key role in the induction of tolerance in pregnancy. This paper reports the identification of a single base pair deletion at position 1597 (1597delC) in exon 3 (encoding the alpha2-domain) of HLA-G on 20 of 272 (7.4 per cent) African American chromosomes, three of 102 (2.9 per cent) Hispanic chromosomes, and none of 134 Caucasian chromosomes. This relatively common frameshift mutation results in amino acid substitutions in all of the residues in the second half of exon 3 including the conserved cysteine at codon 164. An adult individual was identified who was homozygous for this 'null' allele, and a first trimester placenta that was homozygous for 1597delC had no detectable HLA-G1 protein. These data indicate that expression of HLA-G1 protein is not essential for fetal survival.

Adult↗
PubMedDate not supplied

Multiple reward systems and the prefrontal cortex.

Electrical stimulation of the major divisions of the prefrontal cortex, the mediodorsal and sulcal areas, can serve as a reinforcing stimulus. Studies of self-stimulation of the prefrontal cortex have produced behavioral, anatomical and pharmacological evidence that the substrate of these rewarding effects can be dissociated from that subserving self-stimulation of ventral diencephalic sites such as the lateral hypothalamus. Other studies indicate that within the prefrontal cortex itself, self-stimulation of the medial and sulcal divisions can be attributed to dissociable processes. These observations suggest the existence of multiple, largely autonomous prefrontal subsystems involved in reinforcement. This raises the question of the functional significance of such systems, and of their organization. An approach to this problem is to consider the relationship between the behavioral functions of the prefrontal divisions and the characteristics of stimulation-induced reward obtained at each site. Studies of the effects of restricted prefrontal lesions indicate that the medial and sulcal divisions can be dissociated according to their involvement in the control of distinct types of sensory and motor events. Further experiments indicate that damage to each division causes selective deficits in the learning of stimulus-reinforcer and response-reinforcer relations, depending in part on the nature of the reinforcing event. Conditioning experiments further show that the rewarding effects produced by stimulation of these areas are preferentially associated to sensory events which correspond to the functional specialization of each division. These data are interpreted to suggest that different rewarding events and/or different attributes of rewarding stimuli are processed by distinct systems which are reflected by the organization of dissociable self-stimulation pathways.

Animals↗
PubMedDate not supplied

Pulp reactions to restoration of experimentally induced crown fractures.

OBJECTIVES: Reattachment of the avulsed enamel-dentine coronal fragment to the remaining tooth structure has become an accepted clinical alternative to a resin composite build-up for the restoration of crown fractured teeth. Since little knowledge exists as to the pulpal response to this procedure, this study was designed to observe the condition of the pulp following experimentally induced crown fracture and restoration in monkeys. METHODS: Experiments were conducted in eight young green Vervet monkeys (Cercopithecus aethiops). In all, 64 fractured incisors were investigated. Light microscopic examination of pulp tissue specimens was carried out after 3 months of observation. RESULTS: The evaluation was restricted to specimens having a fracture plane within 2 mm of the pulp and no pulpal exposure. In general, pulp tissue was well preserved irrespective of the restorative procedure. Even if the restoration or the bonded tooth fragment had been lost during the follow-up period, the pulp generally remained in good condition. Inflammatory infiltrates where seen in only a few specimens and then as clusters of mononuclear leukocytes. Hard tissue repair was frequently observed and displayed various configurations from isolated hard tissue deposits to areas of extensive hard tissue repair in the coronal portion of the pulp. Pronounced hard tissue repair and occurrence of inflammatory cell infiltrates correlated with the presence of stainable bacteria on the fractured dentine surface. CONCLUSIONS: In the absence of direct exposure, reparative dentine is a frequent feature of the pulp's response to crown fracture and restoration with composite or reattachment of the crown fragment with dentine bonding. These restorative procedures appear to ensure continued function of the underlying pulp.

Animals↗
PubMedDate not supplied

A 6p22 reference map of leukocyte DNA: exclusion of rearrangement in four cases of atypical haemochromatosis.

We describe a 4 Mb reference map of the haemochromatosis gene region in leukocyte DNA from seven controls and four atypical haemochromatosis patients. Three patients had normal coding sequence for HFE, the candidate gene for genetic haemochromatosis (GH). The fourth patient had classical GH but was heterozygous for Cys282Tyr with otherwise normal coding sequence. The genomic DNA was mapped by pulsed-field gel electrophoresis (PFGE) using five rare-cutting enzymes. Seventeen probes including HFE were positioned on the map. Despite proximity to the highly polymorphic major histocompatibility complex (MHC), no polymorphism was observed in the control group with these telomeric probes. Furthermore, major rearrangement of the HFE region was excluded as a mutation contributing to iron overload in these atypical patients. Maps of cloned DNA are linked through genes and other probes to this reference map of the HFE region in uncloned genomic DNA.

Chromosomes, Human, Pair 6↗
PubMedDate not supplied

Contribution of heating, ventilation, and air conditioning airflow and conversation to the ambient sound in a neonatal intensive care unit.

Sound reduction strategies in the neonatal intensive care unit (NICU) must focus on the sources of excessive sound. We studied the relative contribution of personnel conversation and heating, ventilation, and air conditioning (HVAC) airflow by measuring several parameters of sound during four environmental conditions: unaltered ambient sound, HVAC airflow off, HVAC airflow and conversation off, and conversation off. All measurements were decreased by interrupting HVAC airflow and conversation. The reduction was greater when conversation was stopped. The method of sound reduction did not change the frequency distribution of sound. Attention to personnel conversation may be effective in lowering sound exposure in the NICU. The contribution of HVAC airflow should be measured in new constructions and renovations.

Air Conditioning↗
PubMedDate not supplied

Sound transmission into incubators in the neonatal intensive care unit.

OBJECTIVE: To measure the attenuation of sound by modern incubators. STUDY DESIGN: LEQ, LMAX, LPEAK, and frequency distribution were measured simultaneously inside and outside two recent model incubators. RESULTS: The attenuation of sound (outside minus inside) was 15 to 18 dBA with the motor off and 4 to 8 dBA with the motor on. There was a significant difference between incubators in their attenuation of sound. Octave band analysis showed attenuation in frequency bands of > 31.5 Hz with the motor off. With the motor on, the sound level inside the incubator was higher than outside at frequency bands of < 250 Hz. CONCLUSION: Caring for infants inside modern incubators reduces "averaged" sound exposure to levels near those recommended for the neonatal intensive care unit. Lower frequency sounds are louder inside the incubator and arise from the incubator motor.

Humans↗
PubMedDate not supplied

Outcomes analysis of verbal dyspraxia in classic galactosemia.

PURPOSE: This study evaluates a genotype/phenotype relationship between developmental verbal dyspraxia (DVD) and the common, missense mutation of the galactose-1-phosphate uridyltransferase gene, Q188R, in patients with classic galactosemia (G/G). METHODS: As part of this study, we devised a questionnaire for "speech problems" to be completed by the patient\'s clinician. To validate the questionnaire and determine its accuracy in detecting DVD, we analyzed questionnaire responses for 21 patients by testing them independently and directly for DVD through a speech pathologist blinded to the patients' genotype. RESULTS: We found that the questionnaire had a sensitivity of 0.56 and a specificity of 0.75. We then calculated the prevalence of DVD for a larger set of 113 patients with G/G galactosemia whose biochemical phenotype, molecular genotypes, and clinical status were known. The prevalence of "speech problems" from raw data were 50 of 113 (44.2%). After adjusting for misclassification, 43 (38.1%) were classified as cases of DVD. Using multivariate, logistic, regression analyses we found a significant interaction between genotype and mean red blood cell (RBC) galactose-1-phosphate (Gal-1-P). When corrected, using mean RBC Gal-1-P < h 3.28 mg%, the Q188R/Q188R genotype was the best predictor of DVD. There was a significant risk (odds ratio = 9.6, p = 0.0504) of having DVD associated with homozygosity for Q188R compared with other genotypes. CONCLUSIONS: We conclude that homozygosity for Q188R mutations in the GALT gene is a significant risk factor for DVD. However, poor metabolic control obviates this relationship as indicated by RBC Gal-1-P greater than 3.28 mg%.

Apraxias↗
PubMedDate not supplied

Food, health, and the urban environment.

Both the production and the consumption of food should help reduce the risk of food-borne and non-communicable disease and prevent micro-nutrient deficiency. That a safe healthy diet can also be environmentally sound is a bonus. Growing foods that promote health in an environment near to consumers can contribute to reduced fuel consumption, pollution, transport and packaging costs, and can promote biodiversity. This approach can also help create local jobs, improve access to food, and promote a sustainable environment. The World Health Organization recommends an intake of more than 400 grams of vegetables (in addition to potatoes) and fruit every day because this helps prevent non-communicable diseases and micro-nutrient deficiency. Therefore, urban food and nutrition policies are needed to promote health through an integrated approach to food, health, and the environment within local communities. The benefits of developing urban food and nutrition policies to help increase local food production include environmental, social, direct, and indirect economic benefits, in addition to the health benefits. These issues are discussed in this paper.

Agriculture↗
PubMedDate not supplied

The effect of head position on the distribution of drops within the nose.

Nose drops are commonly prescribed in the United Kingdom, yet clinicians vary considerably in the instructions they give on how to instill them. This is important because how they are used affects how well they work. In this study, a visual assessment of the intranasal distribution of drops was made using endoscopic examination after the instillation of betamethasone drops containing methylene blue. Drops instilled with the head tipped back were found along the nasal floor and in the nasopharynx, with none visible in the middle meatus or elsewhere on the lateral nasal wall. Drops instilled in Mygind's position or in the praying-to-Mecca position were found predominantly in the middle meatus and on the middle and inferior turbinates. No difference was seen between these two positions in the distribution of drops achieved, and this is likely to lead to similar clinical efficacy. Because Mygind's position is much more comfortable for patients, it should be recommended by all doctors prescribing nose drops.

Administration, Intranasal↗
PubMedDate not supplied

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Awards and Prizes↗