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Biomedical subjects

A Pardo

Publications and source records attributed to A Pardo.

At least 127 records · Page 7Linked to original sources
PubMed1980

Collagen-bound collagenase.

The presence of collagenase bound to collagen extracted and purified from several animal and human sources by a standard procedure has been confirmed by different methods. Polyacrylamide (10%) gel electrophoresis at pH 8.1 of intact or "spontaneously"degraded neutral salt soluble collagen results in the separation of two components: the upper one says at the origin and represents collagen or collagen ragments, whereas the lower protein component contains no collagen, often preserves specific collagenolytic activity, and migrates as a single band in SDS/polyacrylamide electrophoresis. With lower polyacrylamide gel concentration the electrophoretic separation of the two components is less clear. Removal of the lower protein component from collagen solutions by two different methods (TCA-ethanol purification cycles and pepsin digestion) results in concomitant loss of their "spontaneous" instability. Eluates of the lower protein component stimulate the heterologous production of a monospecific antibody capable of inhibiting the collagenolytic activity of homologous crude collagenase preparations. It is suggested that collagen-bound collagenase is not an artifact of the extraction procedure but rather a physiological reality, probably corresponding in the living animal to the enzyme closely associated with extracellular collagen fibers, revealed by immunohistochemical methods.

Animals↗
PubMed1979

Potassium depletion in aged patients: an evaluation through red-blood-cell potassium determination.

Depletion of potassium is common in old people and is due to abnormal urinary excretion (misuse of diuretics, chronic pyelonephritis), to increased faecal elimination (misuse of laxatives, chronic diarrhoea) or to inadequate dietary intake. In a series of 90 elderly patients whose potassium status was investigated, the main manifestations of potassium deplition were weakness, increased sensitivity to digitials, impaired glucose tolerance and mental confusion. Potassium depletion can be most easily demonstrated by measurement of red-cell potassium levels; this method provides a valuable indication of the intracellular potassium content.

Aged↗
PubMed1975

The presence of collagenase in collagen preparations.

The frequently observed instability of neutral salt solutions of native collagen extracted from various sources and partially purified by standard procedures has been studied by disc electrophoresis in polyacrylamide gel and by electron microscopic examination of segment long spacing crystallites. The phenomenon has revealed time and temperature dependency, pH optima near neutrality, and inhibition by sodium EDTA and serummin addition, collagen breakdown has been found to be quantitatively related to the state of aggregation of the substrate, being more marked in reconstituted collagen gels than in collagen in solutionma typical pattern of animal collagenase degradation of native collagen into two fragments designated as TC-A and TC-B has been observed under certain conditions. It is concluded that the degradation of native collagen in neutral salt solution is due to a specific collagenase, and that this enzyme probably remains bound to collagen throughout the process of extraction and partial purification. Experiments with gelatin suggest that, in addition to collagenase, a nonspecific proteolytic activity may also be present in collagen preparations.

Animals↗
PubMed1975

[Ethiology and pathology of low birth weight infants in our hospital (author's transl)].

A retrospective study on 834 newborns of low birth weight, 2,500 grs., or less over a total of 19,869 live newborns during the year 1973 is presented. They are divided into low birth weight compatible with gestational age and low birth weight for gestational age when birth weight is lower than 10th percentile of Lubchenco's chart. Each of these groups is subdivided in three parts: First part refers to coincidence of frequence of different ethiologic factors or causes of low birth weight (maternal, gestational, placental and termination of pregnancy). The second part has its object in the clinical appraisal of the state at birth of these low birth weight newborns (fetal-obstetric evaluation of Velasco-Cándano, neonatal Apgar scoring, respiratory evaluation by Silvermann-Andersen and evaluation of external maturaty according to Usher). Third part of the study relates the morbility-mortality rate, need for admission, presence of congenital anomalies and autopsy findings in each of these groups of low birth weight newborns. Relation among all these estimated parameters is made presenting some conclusions reached.

Apgar Score↗
PubMedDate not supplied

Uterine lipoleiomyomas: US and CT findings.

Fatty tumors of the uterus are rare. The reported incidence differs, from 0.03% to 0.2%. Two new cases of uterine lipoleiomyoma are presented with ultrasound, computed tomography, and pathologic correlation. To date, only seven cases have been reported in the radiologic literature.

Female↗
PubMedDate not supplied

Exposure to benzene in fuel distribution installations: monitoring and prevention.

The authors used a mixed-effects model on a cohort of 258 randomly chosen workers in 7 fuel-distribution facilities to examine the association between airborne benzene exposure and task and timing factors. During an 8-y period, 692 repeated personal measurements were performed. Filler task, warm month, Tuesday, credit day, and time period (1992-1996) were associated significantly with higher exposures to benzene. The authors controlled for the time period, and task type strongly affected the between-worker variance; therefore, two exposure groups (i.e., fillers and nonfillers) were adequate for purposes of exposure grouping strategy. Timing factors (after controlling for task and period effects) strongly affected the high within-worker variance (> 2 than between-worker variance). Long-term exposure would be better represented if the sample was stratified by warm/nonwarm months and if measurement days were selected randomly.

Adult↗
PubMedDate not supplied

[Thymectomy in myasthenia gravis: long-term evolution and predictive factors].

INTRODUCTION: Thymectomy is a surgical procedure which is generally accepted for treatment of myasthenia gravis. OBJECTIVE: To describe the long-term evolution of 217 myasthenic patients who had had thymectomies in the Hospital Clinico Quirurgico Hermanos Ameijeiras in La Habana, Cuba. PATIENTS AND METHODS: We determined the stage of evolution of 217 patients, followed-up periodically, with an average observation time of 83.4 months and an interval of between 5 and 155 months. We also studied the frequency of remission and the influence of a group of variables on this, as well as the mortality and its causes. RESULTS: The clinical state of the patients was: remission 77 (35.4%); pharmacological remission 45 (20.7%); significant improvement 70 (32.2%); the same or worse 5 (2.3%); deaths 11 (5%), and unknown 9 (4.1%). The cases with thymomas had a more unsatisfactory course than the other patients, with fewer remissions and greater mortality. Over the first five years of the evolution of the disease, there was a 30% rate of remission; after five years this rose to 35-40% and after ten years reached 47%. The age of the patient, duration of symptoms, histology of the thymus, age of onset of the disease and duration of follow-up did not have any significant effect (p < 0.05) on the long-term evolution of the thymectomy, in contrast to the gravity of the condition according to Osserman's scale. CONCLUSION: The results of thymectomy, in our study were good and similar to the results reported in the international literature.

Adult↗