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Biomedical subjects

A Nishimura

Publications and source records attributed to A Nishimura.

At least 253 records · Page 14Linked to original sources
PubMed1979

The choledochoscopic and histopathological grading of choledochitis.

The choledochoscopic photographs of 91 and 119 patients with cholecystolithiasis and choledocholithiasis respectively were reviewed on the basis of endoscopic grading. By analogy with the system of the grading, the diseases were also divided into four histopathological gradings. Choledochoscopy provides an alternative approach to the diagnosis of choledochitis, which is also corroborated by the histopathological data in calculous diseases.

Adult↗
PubMed1979

Amplification and characterization of the proline transport carrier of Escherichia coli K-12 by using proT+ hybrid plasmids.

A previous report [Motojima, K., Yamato, I. & Anraku, Y. (1978) J. Bacteriol. 136, 5-9) described the characteristics of mutants (proT-) of Escherichia coli K-12 that are defective in proline transport carrier activity. Two hybrid plasmids from the Clarke and Carbon colony bank were found to complement the mutation by conjugation and transformation. Analysis with restriction endonucleases showed that both plasmid DNAs carried the same fragment of the E. coli chromosome. A polypeptide with a molecular weight of 24,000, specifically coded for by the proT+ plasmid, was identified in the cytoplasmic membrane by using a double-isotope labeling method in a minicell system. The strain harboring the proT+ plasmid has 6 times as much proline transport carrier as the wild strain. This amplification of the carrier makes it possible to measure proline binding to the carrier by microequilibrium dialysis. Detailed analysis of binding indicated that the maximal amount of proline bound to the carrier is 0.70 nmol/mg of protein of the cytoplasmic membrane of the amplified strain. From this value and the assumption that the carrier has one binding site per minimal molecular weight of 24,000, the content of the proline transport carrier in the cytoplasmic membrane was estimated to be 1.7%.

Bacterial Proteins↗
PubMed1979

Nucleotide sequence of Escherichia coli K-12 replication origin.

From subfragments of an EcoRI fragment (9 kilobase pairs) that contained the replication origin of the Escherichia coli chromosome and had been cloned as a recombinant with a nonreplicating DNA fragment coding for ampicillin resistance, small derivative plasmids were constructed. The smallest of these, pTSO151, contained a segment of 463 base pairs as the chromosomal component. Another plasmid, pSY134, constructed from BamHI digests of the EcoRI fragment and mini-F(pMF21), contained a region of 422 base pairs identical with a corresponding region in pTSO151. We conclude that the replication origin of E. coli chromosome is located within this 422-base-pair segment. The nucleotide sequence of this segment is presented.

Base Sequence↗
PubMed1978

Murein-lipoprotein of Escherichia coli: a protein involved in the stabilization of bacterial cell envelope.

Two independent mutants of Escherichia coli lacking murein-lipoprotein have been found. One mutant whose mutation was named lpo was subjected to detailed analyses. The absence of both bound and unbound lipoproteins was shown by electrophoretic analysis of 14C-arginine labelled membrane proteins of the mutant. Nor was serologically cross-reacting material detected in the mutant by the Ouchterlony-method. Sequestering magnesium from mutant cell suspensions by ethylenediaminetetraacetic acid caused cell lysis, which was prevented in the presence of 0.5 M sucrose. Incubation in culture media at a very low level of magnesium resulted in the formation of blebs in the mutant. Examination of mutant cells by electron microscopy showed that the outer membrane of the mutant was uneven with small irregular protuberances, some of which pinched off forming vesicles of various sizes. Phosphotungstate used for negative-staining penetrated into the periplasmic space of the mutant cells. The mutants leaked a considerable fraction of their periplasmic enzymes. These physiological and morphological alterations in the lipoproteinless mutant suggest that murein-lipoprotein helps to maintain the outer envelope structure by connecting the outer membrane with murein so that the outer membrane may fulfil its physiological functions as a barrier to the environment.

Electrophoresis↗
PubMed1978

Successfully resected carcinoma of the bifurcation of the main hepatic ducts--report of two cases.

Two cases of carcinoma of the bifurcation of the main hepatic ducts are presented. The tumors were successfully removed by resection of the bifurcation in one case, and by left hepatic lobectomy in the other. Some other 38 cases of the same condition collected from the world literature are briefly reviewed and discussed with particular reference to their surgical interventions.

Adenocarcinoma, Papillary↗
PubMed1977

Treatment of carcinoma of the biliary system.

A series of 23 patients with carcinoma at the hilus of the bile duct were studied. Fourteen of the 23 patients underwent surgical resection. Nine of the 14 patients had a curative resection. Six patients are alive after a follow-up period of five to 25 months. The remaining five patients had noncurative resection, and four of them died from metastatic carcinomas during the next 14 months. One patient who had a left hepatic lobectomy is still alive eight months after operation. The other three patients with advanced carcinoma at the hilus of the bile duct were treated by intraoperative radiotherapy. A single dose of 3,000 rads was emitted directly to the tumors. The remaining six patients were treated by drainage procedures only. Four patients with advanced carcinoma of the gallbladder had received radiotherapy. Six of the eight patients who were treated by radiotherapy are alive, although the follow-up period extends to 11 months only.

Adult↗
PubMed1975

Carcinoma of the cystic duct: case report.

A case of carcinoma of the cystic duct is presented and comprehensive review of the literature was provided. Since no characteristic clinical signs are present, the diagnosis can only be made incidentally at the time of laparotomy for non-visualizing gallbladder. Even then histological study of the resected specimen is mandatory. In our present case after the confirmation of the diagnosis the second look exploration was done. However, careful examination of the bile duct system failed to find evidence of carcinomatous involvement suggesting that carcinoma found in the cystic duct was of a primary and not a secondary invasion. The present case constitutes the nineteenth case which meets completely the criteria proposed by Farrar. Since the only hope or cure lies with the early diagnosis of the disease, early exploratory laparotomy and prophylactic removal of nonfunctioning or calculous gallbladder are recommended.

Adenocarcinoma, Papillary↗