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Biomedical subjects

A Neri

Publications and source records attributed to A Neri.

At least 271 records · Page 15Linked to original sources
PubMed1982

Intravascular leiomyomatosis.

Two cases of intravascular leiomyomatosis are described. In 1 case, a 66-year-old woman was seen with an extension of the tumor to the myometrium, cervix, and left parametrium and with radiologic evidence of tumor extension to the left external and common iliac veins and the inferior vena cava; subsequently, the tumor invaded the left renal, suprarenal and ovarian veins, hepatic veins, and the right atrium, causing severe heart failure and death. The second case is that of a 28-year-old woman with an advanced tumor in the interior vena cava, right atrium, and ventricle who died during cardiac surgery. Review of the literature is presented, with special comment on the management of this disease entity.

Adult↗
PubMed1982

Placenta previa. The ultrasonographic placental localization and its influence on the mode of delivery.

In a group of 168 patients who presented with third-trimester bleeding, ultrasonographic placental localization did not provide either positive or negative false results concerning the placental site. In a group of 47 patients for whom an ultrasonographic diagnosis of placenta previa was established, an erroneous typing was done in 4 (8.5%). Of these four, one (2.1%) underwent an elective cesarean section instead of being allowed a vaginal trial of labor.

Delivery, Obstetric↗
PubMed1982

Lymphocyte enzymes in the detection of Niemann-Pick Carriers.

An improved method for the detection of Niemann-Pick disease type A and B carriers is described. Niemann-Pick disease is a genetic disorder characterized by the accumulation of sphingomyelin in several organs, among them spleen, liver and brain. A deficiency in sphingomyelinase activity is the characteristic defect in this lipid storage disorder, and the decreased enzyme activity in these types can be demonstrated in various cells and tissues. Low activity levels of enzymes were found in leukocyte fractions of peripheral blood from patients screened for Niemann-Pick disease. In heterozygote patients levels were within the lower range of control values. We therefore determined sphingomyelinase activity in discrete cell types isolated by two different methods from the total leukocyte fraction. In normal subjects sphingomyelinase activity is five to ten-fold higher in lymphocytes than in granulocytes. Monocytes have an intermediate level. pH profile and stability to heat inactivation were tested in separated lymphocytes and granulocytes and were found to be insignificantly different. Detection of carriers for Niemann-Pick type A and B by using separated lymphocyte as enzyme source, overcomes the difficulty of overlapping values with normal controls and serves as an excellent tool to detect heterozygotes.

Genetic Carrier Screening↗
PubMed1981

Phenotypic drift of metastatic and cell-surface properties of mammary adenocarcinoma cell clones during growth in vitro.

We have examined cell clones obtained from a 13762 mammary adenocarcinoma tumor and its spontaneous lung metastasis for phenotypic stability during serial culture passage in vitro. Two clones that varied markedly in their metastatic properties were chosen for further examination. One of these clones (MTC) obtained from the parental transplanted tumor initially failed to metastasize within 23 days post-injection s.c. but gained the ability to form spontaneous pulmonary metastases after several serial passages in vitro. Another clone (MTLn3) derived from a spontaneous lung metastasis was initially higher metastatic from short-term culture, but lost the potential to form large numbers of spontaneous lung metastases with long-term culture. In contrast to MTA, clone MTLn3 displayed lymph-node metastasis, and the frequency of lymph-node involvement increased when late-passage cultures of MTLn3 cells were assayed in vivo. Both clones from late-passage cultures produced larger tumor sizes at the primary (mammary fat pad) injection sites compared to early passage cells. The morphologies of MTC cells changed with serial tissue culture passage, while the morphologies of MTLn3 cells did not change. The display of fibronectin on MTC cells by immunofluorescence did not change with culture passage; fibronectin was not detected in cultures of clone MTLn3. Fibronectin was also found on MTC cells by cell surface labelling using lactoperoxidase-catalyzed iodination-sodium dodecylsulfate polyacrylamide gel electrophoresis-autoradiography. Iodination of fibronectin on MTC cells did not vary with culture passage, and as in immunofluorescence experiments it was not detected on MTLn3 cells. There was a decrease in exposure of certain cell surface proteins on MTC cells with culture passage, but we did not detect modifications with this procedure that correlated with culture passage of MTLn3 cells. We conclude that prolonged culture in vitro can result in modifications fo metastatic and cell-surface properties of tumor cell clones.

Adenocarcinoma↗
PubMed1981

Recurrent placenta previa.

Among a group of 33 135 women who delivered at Beilinson Medical Center, Israel, during the years 1968 to 1978, recurrent placenta previa occurred in 3.2% of the women, i.e., 6 times higher than the incidence of placenta previa in the general population (0.5%). Analysis of the data revealed that women over 36 yr of age with a history of placenta previa are at risk for recurrent placenta previa. Maternal and perinatal morbidity and mortality were similar in cases of single and recurrent placenta previa.

Adult↗
PubMed1981

Ph1-negative T lymphocytic colonies in agar cultures of peripheral blood in chronic myeloid leukemia.

T lymphocytic colony formation by peripheral lymphocytes separated by discontinuous albumin gradient centrifugation was evaluated in 8 patients with Philadelphia (Ph1)-positive chronic myeloid leukemia (CML). Colonies were obtained using a liquid-on-agar culture system recently introduced (PHA overlayer-leukocyte feeder layer assay) which has been shown to be simple and reliable. The pattern of colony growth in CML and in normal controls was similar, the peak ranging from the 4th to the 6th day. Also the morphological aspects of colonies did not differ in the two groups. Cells recovered from CML lymphocytic colonies were shown to belong to T cell lineage, as they are able to form spontaneous E-rosettes and to respond to mitogenic stimulation in vitro. In contrast, cells recovered from all other cultured fractions failed to display these properties. Cytogenetic analysis showed that T colony cells were Ph1-negative whereas the chromosome anomaly was found in nonlymphoid colonies of the same patients, thus suggesting a nonclonal origin of T lymphocytes in CML.

Agar↗
PubMed1981

Reduced natural killer cell activity in non-hodgkin's lymphoma.

Natural Killer (NK) activity is investigated in 20 patients with non-Hodgkin's Lymphoma (NHL) and in 20 healthy subjects. Peripheral blood lymphocytes are used as effector cells, and 51Cr labelled K562 myeloid cells as targets. Experiments are carried out either at effector/target ratio of 20:1 or 40:1, in 4 hrs Cr released assay. As compared to controls, a reduced NK activity is found in NHL patients, but that difference is statistically significant only for the patients in active disease. Thus, patients in clinical remission display a better preservation of NK function. It is suggested that the study of NK function might offer a helpful tool in following the clinical course and the efficacy of therapy in NHL.

Adult↗
PubMed1981

[Infrastructure for the development of research applied to the health services].

The author considers that the partial views given by research on health services are artificial creations arising out of the needs of specialization. He feels that the research being done is frequently irrelevant, and is prompted only by personal, group or institutional considerations. He points out that a proper understanding of the problem demands greater integration of the academic and the services groups. Some of the advantages of this would be to create favorable conditions in which basic, clinical, epidemiological and administrative research could be conducted, to use research projects for teaching purposes, and to broaden the perspective of administrators and academics by showing them the needs of each of those fields at first hand.

Academies and Institutes↗
PubMed1981

Distribution of fibronectin on clonal cell lines of a rat mammary adenocarcinoma growing in vitro and in vivo at primary and metastatic sites.

With the use of a rat 13762 mammary adenocarcinoma tumor, we have examined the relationship between cellular fibronectin (FN) expression and ability to metastasize spontaneously to regional lymphatic and distant blood-borne sites. This model is based on the isolation and establishment of cell clones from primary parental tumor and from spontaneous metastases that show differing metastatic potentials when implanted s.c. into the mammary fat pads of syngeneic female Fischer 344/CRBL rats. Cellular FN expression was determined in tissue culture as well as primary and secondary tumor sites, utilizing: (a) indirect immunofluorescence microscopy with a specific anti-rat FN antibody (in vitro and in vivo grown cells); (b) competition radioimmunoassay for cell-released FN (in vitro grown cells); and (c) surface labeling by radioiodination-sodium dodecyl sulfate-polyacrylamide gel electrophoresis-autoradiography for cell surface-bound FN (in vitro grown cells). Tissue culture-grown parental tumor clones displayed FN at their cell surfaces. At confluency, they expressed higher quantities of FN at their peripheries and in fibrillar structures between adjacent cells and released greater amounts of this glycoprotein. Lung metastases-derived tumor clones released negligible amounts of FN by radioimmunoassay and failed to express detectable amounts of FN by indirect immunofluorescence and cell surface-labeling techniques. However, when parental tumor- and metastasis-derived clones of widely different metastatic potentials were carefully examined for FN expression and release, there was no obvious relationship between metastasis and FN expression or release in culture or display in tumors at primary or secondary sites. The results suggest that expression or release of FN per se is not a determinant of metastasis, although it may be a factor in certain steps of the metastatic sequence.

Adenocarcinoma↗
PubMed1980

[Present views of lymphocyte growth and differentiation].

In recent years, a large body of information has been accumulated on the origin and differentiation of lymphocytes. According to the present state of knowledge, their ontogeny can be outlined as follows: the pluripotent stem cell originates the lymphatic progenitors, which undergo a series of differentiation events within the microenvironment of the primary lymphatic organs (bone marrow and thymus). Thus lymphatic precursors appear, which finally give rise to mature T- and B-lymphocytes. These events are accompanied by distinct changes in cell markers, mostly surface markers, which have been clearly documented. Some controversy does exist as to whether T and B lineages stem from a common lymphatic progenitor, since recent evidence suggests that B lymphocytes, but not the T, might share a common progenitor with myeloid lineages (hemocytoblast). T-cell maturation starts in the thymic cortex, and under the influence of the soluble thymic factor(s) it progresses in the several post-thymic locations, until final maturation. This process goes from immature (To) to semi-mature (T1) and mature (T2) lymphocytes, these latter mostly characterized by the absence of TdT and by the property to form E-rosettes with SRBC and respond to mitogens. Circulating mature T-lymphocytes display a considerable degree of heterogeneity in terms of functional properties; in this way, various T-subpopulations have been identified on the ground of recognized different properties. The sequence of maturational events of B-lineage takes place within the bursa-equivalent sites. This includes an antigen-independent stage with early B precursors (B0 - B1 - B2), and an antigen-driven maturational phase which actually produces the plasma cells. Among the early B-precursors (B-virgin population), memory cells arise following the antigenic stimulation. Also at that stage, B-cells are highly susceptible to tolerance induction. B-circulating lymphocytes are mainly characterized by S-Ig, Fc receptors, and complement receptors.

B-Lymphocytes↗
PubMed1980

Fertility problems associated with cystic fibrosis.

A case of a cystic fibrosis patient with two pregnancies that ended in deliveries at 30 and 29 weeks' gestation, respectively, is presented. The author suggests that the increased life expectancy of cystic fibrosis patients because of therapeutic advances will increase the need to examine the association and effects of fertility in individuals with this genetic disease.

Adult↗
PubMed1980

Subjective assessment of sexual dysfunction of patients on long-term administration of digoxin.

Various data suggest that male patients who have received digoxin on a longterm basis have increased levels of serum estrogen and decreased levels of plasma testosterone and luteinizing hormone (LH). This study was undertaken to investigate the links between the long-term administration of digoxin therapy and sexual behavior, and the effect of digoxin on plasma levels of estradiol, testosterone, and LH. The patients of the study and control group (without digoxin) were of similar cardiac functional capacity and age (25-40 years) and were randomly selected from the rheumatic heart disease patients. A subjective assessment of sexual behavior in the study and control groups was carried out, using parameters such as sexual desire, sexual excitement, and frequency of sexual relations. Personal interviews and a questionnaire were also used for the evaluation of sexual behavior. The findings support the reports concerning digoxin effect on plasma estradiol, testosterone, and LH. The differences in the means were significant. Tests used to evaluate the changes in sexual behavior showed a significant decrease in sexual desire, sexual excitement phase (erection), and frequency of sexual relations in the study group.

Adult↗