Search PubMed⌕ Search

Biomedical subjects

A Naess

Publications and source records attributed to A Naess.

At least 55 records · Page 3Linked to original sources
PubMed1983

The E-rosette test: variations in results using E from different sheep. Effect of FCS and of AET treatment.

Sheep erythrocyte (E) rosette tests were performed with E from 3 different sheep. The E rosette test for "active" T lymphocytes was performed with untreated E. E tests for the quantitation of the total number of T lymphocytes were performed with untreated as well as AET-treated E, in assays with and without fetal calf serum (FCS). Lymphocytes from patients with active and stable multiple sclerosis (MS) and with infectious mononucleosis (IM) as well as from healthy individuals were examined. The "total" E test performed with untreated E from one sheep and lymphocytes from patients with active MS gave lower percentages of rosette-forming cells (RFC) than when E from the other two sheep were used. FCS in the medium, or AET treatment of E partly but not wholly abrogated this difference. The origin of the E is thus of importance for the results of the rosette tests in patients with active MS, even if FCS or AET treatment is used.

Animals↗
PubMed1983

Influence of fetal calf serum on the results of the E rosette test.

The sheep erythrocyte (E) rosette test was performed with and without fetal calf serum (FCS) in the medium, and using untreated and 2-amino-ethylthioisouronium bromide hydrobromide (AET)-treated E. Peripheral blood lymphocytes from 34 normal donors showed no significant difference in E rosette percentage between the tests performed with or without FCS. However, when using untreated E with FCS in the medium, patients with infectious mononucleosis and those with aseptic meningitis had a significantly decreased percentage of rosette-forming cells (RFC) as compared to the test performed without FCS. When AET-treated E were used, addition of FCS increased rosette formation of lymphocytes from patients with infectious mononucleosis, with stable multiple sclerosis and with brain tumours. Rosette formation with AET-treated E was generally increased when FCS was used, whereas decreased rosette formation was more common when FCS was used in combination with untreated E. The fact that significant differences in rosette formation associated with FCS have been found in certain patient groups, calls for caution when comparing results from laboratories that differ in the use or not of FCS.

Animals↗
PubMed1982

Pre-stained slides for the demonstration of rosette-forming cells.

Twenty-one specimens of cerebrospinal fluid (CSF) were examined for T lymphocytes (sheep erythrocyte rosette-forming cells, E-RFC). Conventional wet slide preparations and prestained slides were compared. The mean difference between parallel preparations was 2.5%. Prestained slides are convenient for the examination of RFC. Preparations are easily made without any disfigurement of the cells or disruption of rosettes.

Adolescent↗
PubMed1982

Cerebrospinal fluid lymphocyte subpopulations before and after pneumoencephalography.

Pneumoencephalography (PEG) did not cause significant changes in cerebrospinal fluid (CSF) lymphocyte subpopulations. Sheep erythrocyte rosette-forming cells (E-RFC) percentage was higher in CSF before (84.0) and after (81.1) PEG than in blood (63.7). The percentage of complement receptor bearing cells was significantly higher in CSF (65.3 and 64.6) than in blood (33.1). Fcgamma receptor bearing cell levels were only slightly higher in CSF (35.6%) than in blood (31.5%), and increased after PEG to 43.9%. The high percentages of both E-RFC and complement receptor bearing cells in CSF, suggest that a considerable number of these cells bear both receptors, in contrast to blood.

Adult↗
PubMed1982

Technical aspects of rosette tests for the demonstration of lymphocyte subpopulations.

Lymphocytes from heparinized or defibrinated blood were separated on Lymphoprep and washed in phosphate-buffered saline (PBS) or Hanks' balanced salt solution (HBSS). Defibrination caused a decreased yield of lymphocytes compared to heparin treatment. The cell loss was probably non-selective, as only minor differences in lymphocyte subpopulations were found. However, lymphocytes from defibrinated blood, washed in PBS gave a lower percentage of rosette-forming cells (RFC) in most tests, and higher number of latex-phagocytizing cells. For the stabilization of sheep erythrocyte (E)-RFC, treatment of E with 2-amino-ethylisothiouronium bromide hydrobromide (AET), with addition of fetal calf serum (FCS), and E-RFC without FCS fixed with glutaraldehyde gave similar results, and higher percentages of RFC than the RFC test performed with FCS. Storage of whole blood or separated lymphocytes for 24 h at 4 degrees C generally resulted in a reduction in the percentages of E-RFC, particularly active E-RFC, but not of EA- or EAC-RFC. The ranges of the results were usually wider after storage.

Cell Separation↗
PubMed1982

Effect of AET treatment of sheep erythrocytes on the rosette test in various diseases.

180 patients with neurological or infectious diseases and 90 normal controls were examined for blood T lymphocyte percentage by the standard method using rosette formation with untreated sheep erythrocytes (E) and a technique using 2-amino-ethyl-thio-isouronium bromide hydrobromide (AET)-treated E, with fetal calf serum (FCS) in the medium. Patients with acute meningitis had a decrease and those with infections mononucleosis had an increase in T lymphocyte percentage as measured by both assays. However, patients with active MS and with cerebral tumours had a decrease in T cell percentage by the E but not by the EAET technique. The T lymphocyte percentages of the other 4 patient groups did not differ significantly from those of the controls, regardless of the technique used. In certain diseases, the use or not of AET-treated indicator cells may thus influence the results of the rosette test.

Animals↗
PubMed1982

Cerebrospinal fluid and blood lymphocyte subpopulations in acute aseptic meningitis.

Lymphocyte subpopulations were determined in cerebrospinal fluid (CSF) from 11 patients with acute aseptic meningitis. The patients had a significantly higher percentage of T lymphocytes (as determined by rosette assays for sheep erythrocyte (E) receptor-bearing cells) in their CSF (86%) than in blood (62%). The percentage of complement (C) receptor-bearing cells was also higher in the CSF (47%), and the sum of E and C receptor positive cells was 133%, indicating a considerable number of cells carrying both receptors in the CSF in contrast to in peripheral blood. However, 8 patients with various neurological diseases had a mean of 65% C receptor-bearing cells in their CSF, while 17 others had 78 +/- 9% E receptor positive cells. Meningitis patients had a lower percentage of CSF Fc receptor-bearing cells (EA-RFC) than other patients. These results indicate the presence of a large percentage of CSF lymphocytes with both E and C receptors also in patients without meningeal disease, and suggest the recruitment of such cells into the CSF.

Acute Disease↗
PubMed1982

Enhancement of E rosette formation by incubation of lymphocytes in cerebrospinal fluid dilutions.

Lymphocytes from normal donors were incubated for 4 h at 37 degrees C in dilutions of normal cerebrospinal fluid (CSF) in Hank's balanced salt solution (HBSS), before being tested for "active" and "total" sheep erythrocyte rosette-forming cells (E-RFC). Incubation in CSF dilutions caused an increase in the percentage of both "active" and "total" E-RFC compared to incubation in HBSS. Incubation in HBSS caused a decrease in "total" but not in "active" E-RFC percentages. CSF thus effects an increase in the ability to form E-RFC under suboptimal conditions. No mitogenic effect of CSF was observed in lymphocyte culture experiments.

Animals↗
PubMed1981

Effect of ACTH treatment on CSF and blood lymphocyte subpopulations in patients with multiple sclerosis.

Patients with active multiple sclerosis (MS) were examined for T lymphocytes (E-rosette-forming cells, E-RFC) and IgG concentrations in cerebrospinal fluid (CSF) and in blood, and for other lymphocyte subpopulations in blood, before and after treatment with adrenocorticotropical hormone (ACTH). Patients who responded to treatment had higher initial percentages of CSF T lymphocytes (93.1 +/- 3.0%) compared to patients who showed no clinical improvement (88.2 +/- 3.2%). After treatment, the responders had decreased CSF T lymphocyte levels (83.1 +/- 6.6%) while those of the nonresponders were unchanged (90.2 +/- 2.2%). In blood, T cell counts were unchanged, but "active" T cell levels showed a tendency to increase in responders and decreased significantly in non-responders. The percentage of Fc receptor-positive cells increased after treatment in both responders and nonresponders, and that of complement receptor-positive cells increased slightly in responders, but these changes were not statistically significant. Serum IgG concentrations decreased in eight of nine patients after treatment. CSF IgG concentrations were found to be increased, unchanged or decreased, after treatment with no significant differences between responders and nonresponders. The results suggests an involvement of the T lymphocyte in active MS.

Adrenocorticotropic Hormone↗
PubMed1980

Lymphocyte subpopulations and IgG concentrations in cerebrospinal fluid and blood from patients with myasthenia gravis.

Twelve patients with myasthenia gravis (MG) were examined for cerebrospinal fluid (CSF) T lymphocytes and for peripheral blood lymphocyte subpopulations (E-, EAET-, EA- and EAC-rosette-forming cells). The patients had a significantly increased percentage of CSF T cells (88.2 +/- 8.7%) when compared with controls (78.0 +/- 9.1%). Absolute concentrations of CSF T cells were not significantly increased. In peripheral blood no significant change in lymphocyte subpopulations was observed, but there was a slight leucocytosis in the patient group. The patients had increased CSF IgG concentrations, CSF IgG to protein ratio, and CSF to serum IgG ratio, indicating an intrathecal production of IgG. No oligoclonal bands could be demonstrated in the agarose gel electrophoresis. The two patients with thymoma had anti-muscle antibodies in CSF and serum, with ratios of 1:256 and 1:1024, respectively. Serum IgG levels were increased, but not significantly, in the patient group. These results suggest an involvement also of the central nervous system in some patients with MG.

Adolescent↗
PubMed1980

Lymphocyte subpopulations in peripheral blood from schizophrenic patients.

Patients with acute and chronic schizophrenia were examined for T and Fc receptor-bearing lymphocytes in blood by means of rosette techniques. The patients had normal numbers of peripheral blood lymphocytes. The percentage of T lymphocytes in patients with acute schizophrenia was reduced (60 +/-2%) compared with controls (66 +/- 1%) and patients with chronic schizophrenia (67 +/- 3%). The total number of T lymphocytes was significantly decreased in patients with acute schizophrenia had slightly elevated numbers (1,778 +/- 200 cells/mm3, P less than 0.002). The percentage and total numbers of Fc receptor-bearing lymphocytes were normal in both patient groups. Both immune mechanisms and the neuroleptic drug treatment may be of importance for the observed decrease in T lymphocyte numbers in blood from patients with acute schizophrenia.

Adult↗
PubMed1980

Lymphocyte subpopulations in multiple sclerosis: variations in rosette tests using erythrocytes from different sheep.

Lymphocytes from 34 patients with active and with stable multiple sclerosis (MS) were examined by rosetting techniques. Patients with active disease had a relative lymphopenia (35 +/- 9%). When tested with erythrocytes (E) from one out of three sheep used, patients with active MS had a decrease in E-rosette forming cells (E-RFC) compared to patients with stable disease and with healthy controls. Other tests (active E-, EAET-, EA-, and EAC-RFC) did not disclose any differences between patients and controls. The results suggest that the origin of the E may be of importance in E-RFC tests.

Animals↗
PubMed1980

Lymphocyte subpopulations in peripheral blood and cerebrospinal fluid from patients with acute optic neuritis.

Ten patients with acute optic neuritis (AON) were examined for T and B lymphocytes in blood and cerebrospinal fluid by means of rosette techniques. The percentage of T lymphocytes in blood was significantly decreased (54+/-3%) compared to controls (66+/-2%). Absolute numbers of T lymphocytes, and relative and absolute B lymphocyte concentrations were not significantly different from controls. The cerebrospinal fluid (CSF) T lymphocyte percentage was significantly increased (88.8% compared to 78.0% in controls). The relative CSF immunoglobulin G concentration was elevated in four patients (40%). On agarose gel electrophoresis, bands in the gamma globulin region were found in two patients (20%).

Acute Disease↗
PubMed1979

T lymphocytes in cerebrospinal fluid from patients with neurological diseases.

T lymphocytes were determined according to their ability to form rosettes with sheep erythrocytes, in cerebrospinal fluid (CSF) from 120 patients, and in peripheral blood from 59 patients. Normal CSF contained 74.9 +/- 9.6% T lymphocytes. Increased T lymphocyte percentage was found in CSF from patients with active multiple sclerosis (MS), and Guillain-Barré syndrome, as well as the 2 patients with retrobulbar neuritis and the 1 with subacute sclerosing panencephalitis. Patients with stable MS showed no significant change in CSF T lymphocyte percentage. CSF from patients with viral meningo-encephalitis or meningo-radiculitis had a decreased T lymphocyte percentage. This decrease was also found in the 2 patients with malignant disease and the 2 with presenile dementia. An exceptionally low CSF T cell count (5%) was found in 1 patient with cerebellar ataxia.

Humans↗
PubMed1978

T lymphocytes in peripheral blood from patients with neurological diseases.

Absolute and relative numbers of T lymphocytes in peripheral blood were determined in 19 different groups of patients with neurological diseases, using the E-rosette test. A significantly decreased total number of T lymphocytes was found in the following groups: acute Guillain-Barré syndrome (GBS), active multiple sclerosis (MS), and malignant cerebral tumor. A less pronounced and not significant reduction was observed in patients with amyotrophic lateral sclerosis (ALS). In all other groups of patients normal total numbers of T lymphocytes were found, thus indicating that the decrease in T lymphocyte counts in GBS, MS and malignant cerebral tumors is not due to nonspecific injury of nerve tissue.

Adult↗
PubMed1978

Multiple sclerosis. T lymphocytes in cerebrospinal fluid and blood.

Peripheral blood from 21 patients with active and 19 patients with stable multiple sclerosis (MS), and cerebrospinal fluid (CSF) from 7 of the patients with active disease were examined for T lymphocytes by rosette formation with sheep erythrocytes. Increased numbers of T cells were found in CSF in active disease (94.0 +/- 0.7 compared to 71.3 +/- 4.4% in controls). Decreased relative and absolute numbers of T cells were found in peripheral blood in active, but not in stable disease (mean 47 +/- 3 and 957 +/- 93%/mm3 compared to 66 +/- 2 and 1,381 +/- 93%/mm3 in controls).

Humans↗
PubMed1976

Demonstration of T lymphocytes in cerebrospinal fluid.

Cerebrospinal fluid (CSF) was allowed to drop straight into Hanks's balanced salt solution. After centrifugation the pellet was resuspended and mixed with sheep erythrocytes. The mixture was further handled as in the E-rosette test with peripheral blood lymphocytes. CSF from 20 individuals were investigated, and rosette-forming cells (RFC) were found in all. Six patients with normal fluid had between 46% and 83% RFC. Four patients with multiple sclerosis had increased numbers of RFC (94%-96%). Low numbers of RFC were found in one patient with cerebellar ataxia and in one of two patients with acute viral meningitis. With this technique RFC can be counted even in normal CSF with a 3-ml sample.

Cerebrospinal Fluid↗