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Biomedical subjects

A Murata

Publications and source records attributed to A Murata.

At least 163 records · Page 9Linked to original sources
PubMed1988

Release of pancreatic secretory trypsin inhibitor from human hepatoblastoma cells on stimulation with cytokines.

To determine whether or not human pancreatic secretory trypsin inhibitor (PSTI) is an acute phase reactant released from hepatocytes, we investigated the effects of various cytokines on the release of PSTI from cultured human hepatoblastoma cells. PSTI was synthesized in human hepatoblastoma cells and released on stimulation with various cytokines: interleukin-1, tumor necrosis factor and interferon-beta.

Biological Factors↗
PubMed1988

Effect of aging on collateral circulation via pial anastomoses in cats.

The effect of aging on collateral circulation via pial anastomoses after middle cerebral artery occlusion was investigated in young and aged cats. The reduction of blood flow and electroencephalography power were greater and collateral vessel resistance was higher in the aged group than the young group. Electroencephalography power after the occlusion was correlated with collateral resistance and blood flow in the two groups. These results suggest that collateral function deteriorates with aging and the high vulnerability to ischemic insult in aged brain may be caused in part by the poor collateral function.

Aging↗
PubMed1988

Long-term effects of thyrotropin-releasing hormone and histidyl-proline diketopiperazine on the maturation of homeothermia and mitochondrial enzyme activities in neonatal rats.

To elucidate the mechanism by which TRH and its metabolite, histidyl-proline diketopiperazine (cyclo(His-Pro], act on the maturation of homoiothermy, the chronic effects of intrathecal administration of the peptides on body temperature, serum thyroid hormone levels, and mitochondrial energy-producing enzyme activities were examined in neonatal rats. The two peptides or an equimolar mixture of both were injected intrathecally at a dose of 3, 6 and 9 nmol for 7 consecutive days during the 1st, 2nd or 3rd week of life, respectively. Control rats were treated with saline and they were sacrificed at 6 weeks of age. Although food and water intake were not decreased, body weight gain was slightly reduced in the rats treated with TRH or cyclo(His-Pro) during the 1st and 2nd week of life, whereas the mixture-treated rats showed normal weight gain. Body temperature at 25 degrees C was not different in the TRH- and cyclo(His-Pro)-treated groups, whereas after cold exposure (5 degrees C for 3 h), the groups treated with TRH during the 1st and 2nd week of life had an impaired thermoregulation at 5 weeks of age. Serum T4 and T3 concentrations were similar in all groups, except in the rats treated with TRH during the 2nd week of life; their thyroid hormone levels were slightly reduced. The TRH treatment suppressed mitochondrial cytochrome c reductase and glucose-6-phosphatase activities, whereas cyclo(His-Pro) reduced cytochrome c reductase and malic enzyme activities. In contrast, alpha-glycerophosphate dehydrogenase was enhanced by both treatments.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1988

Purification, characterization and amino-acid sequencing of two pancreatic secretory trypsin inhibitors in rat pancreatic juice.

We purified two pancreatic secretory trypsin inhibitors (PSTI-I and PSTI-II) from rat pancreatic juice. PSTI-I consisted of 61 and PSTI-II of 56 amino-acid residues. Their amino-acid sequences were similar (40 out of 56 amino acid residues of PSTI-II being identical with those of PSTI-I), but PSTI-I and PSTI-II appeared to be translation products of different genes. There was no difference in inhibitory properties between PSTI-I and PSTI-II.

Amino Acid Sequence↗
PubMed1988

Pancreatic secretory trypsin inhibitor immunoreactivity detected in serum-free culture medium of human pancreatic carcinoma cell line, CAPAN-1.

Pancreatic secretory trypsin inhibitor (PSTI) immunoreactivity was detected by a radioimmunoassay in a serum-free culture medium conditioned by a human pancreatic adenocarcinoma cell line, CAPAN-1. Its apparent rate of secretion was 280 +/- 20 fg/cell/24 h, and increased in the presence of fetal bovine serum, but was not affected by a cell density at an early stage of culture. This PSTI immunoreactivity was indistinguishable from authentic human pancreatic PSTI on gel filtration, immunoblot analysis and trypsin affinity chromatography.

Adenocarcinoma↗
PubMed1987

[Instabilities in tactile, visual and auditory sense--application of stochastic catastrophe model].

As a fundamental study on management of the working environment, instabilities in tactile, visual and auditory sense are discussed. The effects of localized vibration on the ability to discriminate the surface roughness are analyzed by means of the stochastic cusp catastrophe model. Also, bistable perception of the ambiguous man/girl figure is modelled in the same way. Furthermore, the instable phenomenon of a pure tone when heard under the masking noise is pointed out and analyzed by the catastrophe model.

Adult↗
PubMed1987

Self-rating depression scales correlated with regional cerebral blood flow in normal volunteers.

In order to investigate possible influences of satisfaction with life on regional cerebral blood flow (rCBF), we studied the relationship between the self-rating depression scale and rCBF in normal middle-aged volunteers. A significant inverse correlation between SDS scores and the rCBF was observed. This result was independent of the influences of risk factors for stroke and age. Our findings suggest that satisfaction in daily life is important in maintaining active levels of brain metabolism.

Adult↗
PubMed1987

Spontaneous growth hormone secretion and plasma somatomedin-C in children of short stature.

We studied 17 short prepubertal children, aged 7.5 to 17.0 years (mean +/- SD: 11.7 +/- 2.4) more than 2.0 SD below the mean height for their age and of delayed bone age (M +/- SD: 8.1 +/- 2.3), to clarify their physiological GH secretory status. The mean concentration of GH (MCGH) was calculated and was compared with the subjects' GH responses to insulin and arginine tolerance tests (IATT) and plasma somatomedin-C (SM-C). The mean 24-h MCGH value was 3.2 +/- 1.3 ng/ml (range 1.6-5.5). The mean peak GH response to the IATT was 13.0 +/- 7.5 ng/ml (range 2.4-33.9). In addition to the two patients with abnormally low GH responses to the IATT, seven with normal responses showed low 24-h MCGH values, a small number of GH pulses and low mean GH amplitude. The mean plasma SM-C in all patients was 0.60 +/- 0.20 U/ml. This was significantly lower than that of age-matched children of normal height (p less than 0.001). The 24-h MCGH was significantly correlated with plasma SM-C levels (r = 0.51, p less than 0.05) and with that of the first three hours of sleep at night (r = 0.84, p less than 0.01). These results indicate that: 1) some short children with normal GH response to pharmacological tests secrete a low amount of GH physiologically and 2) blood sampling during the first three hours of sleep as well as 24-hour sampling is suitable in evaluating the physiological secretion of GH.

Adolescent↗
PubMed1987

[Role of natural killer cells in the antimetastatic effect of defibrinogenation with batroxobin].

The antimetastatic effects of defibrinogenation with batroxobin were investigated in normal mice and in mice with depressed or activated natural killer (NK) cell activity. Batroxobin inhibited the formation of lung metastases after intravenous inoculation of the F10 subline of B16 melanoma. Inhibition of NK activity by treatment of mice with anti-asialo GM1 antibody abrogated the antimetastatic effects of Batroxobin. Conversely, augmentation of NK cell activity by poly I:C plus treatment with batroxobin produced additive antimetastatic effects. Studies on the mechanism of interaction between Batroxobin and NK cells revealed that Batroxobin treatment did not affect splenic NK activity in vitro. From these data, it was found that the antimetastatic effects of batroxobin are dependent on the level of NK activity in the host.

Animals↗