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A Milosevic

Publications and source records attributed to A Milosevic.

At least 19 recordsLinked to original sources
PubMed2004

Epidemiological studies of tooth wear and dental erosion in 14-year old children in North West England. Part 2: The association of diet and habits.

OBJECTIVE: To determine the strength of association (expressed as Odds Ratios) of potential risk factors with erosion and tooth wear in 14-year-old schoolchildren. DESIGN: A random sample of 2,385 children were selected by a stratified two-stage technique based on schools and children. SETTING: Schools in NW England. METHODS: Tooth wear was assessed by one examiner on three surfaces of all 12 anterior teeth (labial, incisal and palatal) and the occlusal surface of all four first molars using a four-point scale. Enamel wear was scored 0, dentine exposure <1/3 scored 1, >1/3 scored 2 and secondary dentine or pulpal exposure, scored 3. A questionnaire enquired about general health, dental health, habits and the frequency of intake of a wide range of foods and drinks. RESULTS: The Odds Ratios for tooth wear on any surface for habits, reflux and certain foods were: bruxism, 1.10; stomach upset, 1.45; pickles 1.86; vinegar 1.36; salt and vinegar crisps 1.33; brown/other sauces 1.57. Similarly, the odds ratios for potentially erosive drinks were: fizzy drinks 1.32; sport drinks 1.58; herbal/lemon tea 3.97. The frequency of intake was bi-modal with 397 children drinking a can per day and 207 drinking two cans per day. A significant number drank acidic beverages at bedtime but this was not associated with dental erosion. CONCLUSION: Although odds ratios greater than unity indicate an association, this was not high for carbonated beverages and many other acidic foods or drinks. Examining at fourteen years may not be ideal, as the determinants of erosion/tooth wear have not acted for long, the indices do not discriminate sufficiently and proportionately few subjects have dentine exposed on smooth surfaces.

Adolescent↗
PubMed2004

Epidemiological studies of tooth wear and dental erosion in 14-year-old children in North West England. Part 1: The relationship with water fluoridation and social deprivation.

OBJECTIVE: The effect of water fluoridation upon dental erosion/tooth wear in the UK has not been investigated. This study aimed to compare the prevalence of tooth wear in 14-year-old schoolchildren in non-fluoridated and fluoridated districts of North West (NW) England. The influence of deprivation and tooth brushing was also investigated. DESIGN: A random sample of 10% of the 14-year-old population in NW England was selected and stratified according to fluoridation status as determined from water authority postcode listings. METHODS: Tooth wear was scored on the labial, incisal and palatal/lingual surfaces of the 12 anterior teeth and the occlusal surfaces of the first molars. Enamel wear was scored 0, dentine was scored 1 or 2, dependent on whether less than or more than a third of the surface had exposed dentine. Secondary dentine or pulpal exposure scored 3. Townsend deprivation scores were gained from residential post codes. RESULTS: A total of 2,351 children were examined, of which 637 (27%) lived in the one fluoridated district of South Cheshire and 1,714 (73%) lived in 11 non-fluoridated districts. Fifty-three per cent of the children had exposed dentine with significantly more males affected than females (p<0.001). In the fluoridated district, significantly fewer children had exposed dentine on labial and palatal smooth surfaces (p<0.001) but no differences were found for incisal and occlusal surfaces. The interaction of fluoridation and tooth brushing twice per day resulted in a significant (30%) reduction in erosion. Smooth surface wear was more prevalent in children resident in affluent areas. CONCLUSION: Children in non-fluoridated districts are 1.5 times more likely to have smooth surface wear compared with children in fluoridated districts. Fluoridation and use of fluoridated toothpaste twice a day provide added protection from dental erosion. The risk of tooth wear is greater with increasing affluence.

Adolescent↗
PubMed2004

Cause for concern?

Explore the source record for details and available documents.

Anti-Bacterial Agents↗
PubMed2000

The post-orthodontic prevalence of temporomandibular disorder and functional occlusion contacts in surgical and non-surgical cases.

This study aimed to assess mandibular mobility and the prevalence of functional occlusal contacts in subjects treated to a Class 1 incisor relationship by fixed orthodontic appliance therapy. Two hundred and thirty subjects participated (mean age=18 years) of whom 42 underwent orthognathic surgery. All subjects were in retention with a mean time of 7 months between debond and examination. Maximal mandibular opening, lateral and protrusive excursions were all significantly reduced in the surgical cases compared to the non-surgical group. Centric and eccentric non-ideal occlusal contacts were not different between surgical and non-surgical groups. Non-working side contacts occurred in 30% of subjects, posterior contacts on protrusion in 20% and RCP-ICP prematurities in 18% of subjects. Non-working side contacts were significantly more frequent in post-graduate cases compared to staff cases (P<0.05). An overbite less than the mean of 2.4 mm resulted in a reduced likelihood of canine guidance on the working side (P<0.001) and an increased frequency of non-working side contacts and posterior contacts on protrusion (P<0.001).

Adolescent↗
PubMed2000

Comparison of three facebow/semi-adjustable articulator systems for planning orthognathic surgery.

Our aim was to measure the steepness of the occlusal plane produced by three different semi-adjustable articulators: the Dentatus Type ARL, Denar MkII, and the Whipmix Quickmount 8800, and to assess the influence of possible systematic errors in positioning of study casts on articulators that are used to plan orthognathic surgery. Twenty patients (10 skeletal class II, and 10 skeletal class III) who were having pre-surgical orthodontics at Liverpool University Dental Hospital were studied. The measurement of the steepness of the occlusal plane was taken as the angle between the facebow bite-fork and the horizontal arm of the articulator. This was compared with the angle of the maxillary occlusal plane to the Frankfort plane as measured on lateral cephalometry (the gold standard). The Whipmix was closest to the gold standard as it flattened the occlusal plane by only 2 degrees (P<0.05). The results of the Denar and Dentatus differed significantly from those of the cephalogram as they flattened the occlusal plane by 5 degrees and 6. 5 degrees (P<0.01), respectively. Clinicians are encouraged to verify the steepness of the occlusal plane on mounted study casts before the technician makes the model.

Analysis of Variance↗
PubMed2000

The importance of antioxidant enzyme and total antioxidant status of patients with acute myocardial infarction on thrombolytic therapy.

It is believed that intensive production of free radicals occurs immediately after reperfusion, and that it explains a series of adverse effects of reperfusion. The aim of the study was to establish the importance of the antioxidative enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX) and the total antioxidant status (TAS) in patients with acute myocardial infarction according to success or failure of reperfusion. Enzyme activities in erythrocyte hemolysate and concentration of TAS in plasma were determined by Randox kits in 50 patients with acute myocardial infarction. No significant differences in TAS between the mean values against either clinical status or the 10 time intervals were recorded. The SOD and GPX values were significantly influenced by the presence or absence of reperfusion (p<0.05). Monitoring of SOD and GPX at ten time points: before administration of streptokinase, 1, 3, 6, 12, 18, 24 hours, 2, 3 and 5 days after administration of streptokinase revealed the lowest SOD levels up to one hour before administration of streptokinase, supporting the fact that the maximum production of free radicals is achieved at that time. GPX values differed for 24 hours according to whether reperfusion was achieved or not. The obtained results suggest that free radical production is increased after reperfusion, and monitoring of antioxidant enzymes may help to evaluate the success of thrombolytic therapy.

Analysis of Variance↗
PubMed1999

Early development and composition of the human primordial plexiform layer: An immunohistochemical study.

The early expression of reelin, calcium-binding proteins (calretinin, calbindin, and parvalbumin), and neurofilament proteins have been explored in the developing central nervous system of human embryos and fetuses during the first trimester of gestation. Our objective has been to determine further the nature, developmental roles, and contributions of the early neurons and fibers of the original subpial neuropil, i.e., the primordial plexiform layer (PPL). In young embryos (4-5 weeks old), neurofilament protein-labeled fibers run through the subpial neuropil of the caudal portion of the neural tube, reaching the mesencephalon rostrally. At this age, calretinin-immunoreactive and calbindin-immunoreactive neurons are also found among cells already detached from the ventricular zone. The expression of neurofilament protein, calretinin, and calbindin follows an ascending caudorostral gradient, reaching the cerebral vesicles by the 6th-7th week of gestation. In the cerebral cortex, this timing coincides with the initial expression of reelin in the PPL. The reelin immunoreactivity throughout the most superficial cellular population of the cortical PPL supports the early genesis of Cajal-Retzius cells, around the 6th week of gestation. After the splitting of the PPL by the formation of the cortical plate (7-8 weeks of gestation), reelin-immunoreactive cells remain only in the newly established layer I. This study proposes that an initial PPL may be a universal feature of the developing central nervous system.

Calbindin 2↗
PubMed1999

Eating disorders and the dentist.

Dentists are likely to encounter patients who have eating disorders. The paper explains the various types and considers both the risk factors, and the psychological and medical complications. The effect on oral health and the principles of dental management are outlined. Dentists have an important part to play in the overall care of these patients.

Adolescent↗
PubMed1999

Calcium signaling molecules in human cerebellum at midgestation and in ataxia.

A fundamental question in brain development is how neurons make the precise topographic connections necessary for function. The hypothesis that transient expression of calcium (Ca2+) signaling molecules may have a role in this process was tested by studying human cerebella at midgestation. In addition, four adult brains, two controls and two from patients with ataxia, were studied as well. The temporal and spatial distribution of intracellular Ca2+ channel/receptors, inositol trisphosphate receptor type 1 (IP3R1) and ryanodine receptor (RyR) and three Ca2+ binding proteins were examined with immunocytochemical methods. A positive immune reaction with all markers of Ca2+ signaling was found in the Purkinje cell layer starting from 17 g.w. (gestational weeks), the youngest age studied. The immune reactions were not homogeneous throughout the extent of the Purkinje cell layer, but instead displayed a 'patchy' appearance in all intrauterine stages. In the adult cerebellum the expression of Ca2+ signaling molecules was homogenous. In the two cerebella obtained from patients suffering from ataxia, a several-fold reduction of immunostaining with IP3R1 was found. Our findings suggest that transient and differential mobilization of intracellular Ca2+ in seemingly homogenous neuronal types may play a role in development of highly organized projection maps of the cerebellar cortex. Moreover, lack of IP3R1 in the diseased brains suggests that internal stores of Ca2+ play an important role in normal function of the cerebellum.

Adult↗
PubMed1999

The occurrence of toothwear in users of Ecstasy (3,4-methylenedioxymethamphetamine).

UNLABELLED: Ecstasy users have reported that dry mouth, jaw tension and tooth grinding were common side effects of its use although the influence of these effects upon toothwear have not been previously investigated. OBJECTIVE: This study aimed to compare incisal and occlusal toothwear in Ecstasy users and a group of non-users of Ecstasy but users of other drugs. METHODS: Groups were established by a snowball peer information network from visitors to the "drop-in" Maryland Centre in Liverpool. Volunteers completed a questionnaire about social life, drug use and diet. Clinical examination for wear on the incisal edges and on canine tips was conducted with a mirror and probe, whereas occlusal wear was recorded in impressions and subsequently scored from stone replica casts. The degree of toothwear was scored according to the criteria of the Tooth Wear Index (Smith & Knight, Br Dent J 1984;157:16). RESULTS: Ecstasy users (n = 30) were compared with non-users (n = 28). Toothwear through the enamel into the underlying dentine occurred in 18 (60%) Ecstasy users but in only three (11%) non-users. The overall mean toothwear score in Ecstasy users was 0.63 compared with 0.16 in non-users (t = 4.34, P < 0.001). Dry mouth was reported by 93% of Ecstasy users whilst 89% stated that they clenched or ground their teeth after taking the drug. Tooth grinding commonly continued into the following morning. Carbonated (acidic) beverages were consumed by 93% of the users with a mean of three cans per "trip". CONCLUSION: The severity of toothwear and the number of teeth affected were greater in Ecstasy users than in a group of non-users. The occlusal surfaces were more commonly affected than the incisal, which may indicate jaw clenching rather than grinding as a feature of Ecstasy-induced muscle hyperactivity.

England↗
PubMed1999

Thickness of acquired salivary pellicle as a determinant of the sites of dental erosion.

Dental erosion shows a typical distribution pattern within the dental arches. Tooth protection from erosion by salivary pellicle has been shown in vitro, but the hypothesis that pellicle may differ quantitatively at sites of erosion has not been investigated. This study aimed to determine the thickness of acquired salivary pellicle within the dental arches, investigate the possible relationship of this thickness to the distribution and severity of erosion within the arches, and confirm the protective effect of pellicle against dental erosion. Eight enamel blocks were produced from each of 5 bovine incisors assigned to five volunteers. Each block was further cut into 2 slabs, producing control and experimental slabs. Pellicle developed on experimental slabs located on 8 intra-oral sites after 1 hr of exposure was stained by "sheep anti-human IgGAM-FITC". Slabs were then visualized, and pellicle thickness measured, by confocal laser scanning microscopy. Eroded enamel lesions were produced in experimental and control slabs by means of pure orange juice. The degree of erosion was quantified by transverse microradiography. Pellicle thickness varied significantly within the dental arches and among individuals. An inverse relationship (r = -0.96, p<0.001) was observed between the degree of erosion and pellicle thickness. Significant differences in erosion were observed between slabs with and those without pellicle. This study has shown that the thickness of acquired salivary pellicle varies within the dental arches, which may be responsible for the site-specificity of dental erosion, and that pellicle does protect the teeth from erosion.

Analysis of Variance↗
PubMed1999

Use of articulators in the United Kingdom by consultant orthodontists in planning orthognathic surgery.

This questionnaire survey aimed to investigate articulator use in orthognathic surgical planning by consultant specialists in the United Kingdom (UK). A total of 205 questionnaires was sent to all consultant orthodontists in the UK. One hundred thirty questionnaires were completed, representing a 63.4% response rate. Consultants had been in their post for a median of 10.5 years (range 0 to 30 years) with a mean of 11.3 (SD 8.4) surgical cases per year. When asked which cases had been planned on a semiadjustable articulator, two thirds of consultants (67.7%) planned maxillary single-jaw procedures, 45.4% planned mandibular single-jaw procedures, and 77.7% planned bimaxillary procedures. While 98.5% of consultants reported access to at least one type of articulator, 14.6% of maxillary single-jaw and 5.4% of bimaxillary surgery was not planned on any articulator. Twice as many consultants who had been in their post fewer than 11 years had been trained on a semiadjustable articulator, compared to those who had more than 11 years of experience. Semiadjustable articulators were the most popular for planning orthognathic surgery. Consultants with less than 11 years of experience completed more surgical cases each year and were more likely to have been trained on a semiadjustable articulator than consultants with more than 11 years of experience. However, no link exists between the age of the consultant and the type of articulator selected for planning; this suggests that more mature consultants have received further training on contemporary articulator systems since receiving their accreditation.

Dental Articulators↗
PubMed1998

Developmental changes in human cerebellum: expression of intracellular calcium receptors, calcium-binding proteins, and phosphorylated and nonphosphorylated neurofilament protein.

Few recent data are available on the development of the precise projection maps of the cerebellar cortex in humans. To address this topic, we studied temporal and spatial distribution of several antigens involved in calcium (Ca)-dependent processes: the intracellular Ca receptors, inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) and ryanodine receptor (RyR); the Ca-binding proteins, calbindin D-28k (CB), parvalbumin (PV), and synaptophysin; and phosphorylated (SMI 31) and nonphosphorylated (SMI 32) forms of neurofilament protein. All antigens were studied in the human cerebellum during intrauterine development. The results of this study show that immunocytochemical markers appeared in the following sequence: CB and both forms ofneurofilament protein were observed at 4-5 gestational weeks (g.w.), PV appeared in the external granular layer and in a few Purkinje cells at 11 g.w., a diffuse immunostaining for IP3R1 and synaptophysin were observed at 13 g.w., whereas RyR was observed at 17-18 g.w. From 24 g.w. on, Purkinje cells expressed all four examined markers of intracellular Ca signaling as well as two forms of neurofilament protein. At the same time, compartmentation of the Purkinje cell layer was detected with three intracellular Ca-signaling molecules (IP3R1, CB, and PV) and with SMI 32. These results indicate that the developmentally regulated expression of antigens studied here may play a role in establishing a highly regular organization of terminal fields in the human cerebellar cortex. Moreover, the initial expression of these antigens is correlated temporally with other developmental processes in the cerebellum, such as cellular maturation, revealed by the immunoreaction to cytoskeletal protein, and synaptogenesis, revealed by immunoreaction to synaptophysin.

Afferent Pathways↗
PubMed1998

Functional occlusion after fixed appliance orthodontic treatment: a UK three-centre study.

This study aimed to assess the prevalence of functional occlusal contacts in cases treated to a Class I incisor relationship by upper and lower fixed appliance therapy. Helkimo's dysfunction indices were used by one examiner to assess mandibular mobility, clinical dysfunction and occlusal status. One-hundred-and-eighty-eight subjects participated in three UK orthodontic centres; Cardiff, Newport and Liverpool. The results show that RCP (CR)-ICP (CO) discrepancies occurred in 33 (18 per cent) cases, non-working side contacts were present in 31 per cent and posterior contacts on protrusion occurred in 44 (23 per cent) subjects. These interferences were significantly more frequent in cases treated by postgraduate students compared with those treated by staff clinicians (P < 0.05). An overbite greater than 2.4 mm resulted in a highly significant reduction in the occurrence of non-working side contacts and posterior protrusive contacts (P < 0.001). The prevalence of functional occlusal discrepancies in this UK sample of orthodontic cases is similar to that reported in the American literature.

Adolescent↗
PubMed1998

Toothwear: management.

The management of toothwear encompasses a broader approach than the active treatment of the problem, taking in as it does both passive and active interventions. This paper gives guidelines on how to assess and broadly manage toothwear rather than specific examples of treatment.

Bruxism↗
PubMed1997

Sports supplement drinks and dental health in competitive swimmers and cyclists.

AIM: To assess dental status and sports supplement uptake in swimmers and cyclists. To determine chemico-physical properties of the most popular sports drinks. DESIGN: Descriptive, prevalence study of tooth wear and caries experience. Questionnaire analysis of sports drinks usage. SETTING: Two public swimming pools in Liverpool and three cycle clubs in North West England. SUBJECTS AND METHOD: A convenience sample of swimmers and cyclists was examined for caries and tooth wear. A questionnaire ascertained which sports drinks were consumed and their pattern of consumption. pH and titratable acidity, concentrations of calcium, phosphate and fluoride, and viscosity were analysed. Salivary flow rate in response to these drinks and water was also determined. RESULTS: 25 swimmers and 20 cyclists participated. Caries experience and tooth wear into dentine (excluding incisally exposed dentine) was significantly more frequent among cyclists (P < 0.05). Cyclists had significantly more upper palatal wear (P < 0.001). Pattern of sport drink consumption between the two groups was significantly different (P < 0.001). pH range of the most popular sport drinks was 2.4-4.5. Salivary flow rate after a 1-minute rinse was significantly lower (P < 0.05) with one drink (0.47 ml/min) and water (0.41 ml/min) compared with the other drinks. CONCLUSIONS: An association between caries or erosive tooth wear and sport drink consumption was not found. However, the erosive potential of sport drinks is real and must be borne in mind as an aetiological factor for erosion in young people.

Adolescent↗
PubMed1997

Initial development of gamma-aminobutyric acid immunoreactivity in the human cerebral cortex.

The development of cortical cells immunoreactive for gamma-aminobutyric acid (GABA) was studied in human cerebral cortex in the first trimester of gestation (from 4 to 13 gestational weeks; g.w.). The first GABA-immunoreactive (IR) cells were observed at 6.5 g.w., i.e., before the appearance of the cortical plate, which gives rise to a majority of the adult cortical layers. GABA-IR cells were found initially in the telencephalic wall, where a lateromedial gradient in the density of GABA-positive cells was observed at this early developmental time point, but not at later stages. At 7 g.w., as the cortical plate emerged in the ventrolateral region of the cerebral vesicle, GABA-immunoreactive cells were found dorsal and ventral to the developing cortical plate. At this stage, immunoreactivity was also observed in the other transient developmental zones of the cortical anlage: in the subplate layer and in the intermediate, subventricular and ventricular zones. From 8 to 9 g.w. and continuing throughout the end of the studied period (13 g.w.), GABA-IR cells were distributed throughout the full width of the telencephalic wall, and, at 13 g.w., the newly formed subpial granular layer contained GABA-immunoreactive cells, as well. However, the predominant sites for GABA immunoreactivity remained the prospective layer I and the subplate. The population of GABA-positive cells described here was not immunoreactive for glial fibrillary acidic protein (GFAP) at any gestational age examined and, therefore, probably represents GABA-containing neurons. The observation that GABA-IR neurons appear in human developing cortex slightly before the cortical plate formation and beginning of synaptogenesis (6.5 g.w.) suggests that GABA plays an important role in the initial organization of the developing human cerebral cortex.

Cerebral Cortex↗