Search PubMed⌕ Search

Biomedical subjects

A Mazzone

Publications and source records attributed to A Mazzone.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

[The central nervous system and inflammation: the role of opioid peptides].

Inhibition of human granulocyte aggregation was observed in the presence of morphine in a naloxone stereoselective way, while the opioid peptides were ineffective. In comparative studies Ca(+)+ (A23187) and dynorphin 1-9, per se, induced stimulation of arachidonic acid metabolites from granulocytes. In this regard dynorphin 1-9 may function as a mediator between the central nervous system and the inflammation.

Arachidonic Acids↗
PubMedDate not supplied

[Diphenylhydantoin induces an alteration in immune system cellular functions: an in vitro study of platelet and granulocyte aggregation].

In vitro experiments were performed in order to clarify the influence of phenytoin on human granulocyte and platelet function. Therapeutic anticonvulsant concentrations of the drug significantly reduced the granulocyte aggregation produced by formyl-methionyl-leucyl-phenilalanine; phytoemoaglutinin-induced granulocyte aggregation was unaffected by phenytoin. In the same range of concentrations the drug was able to inhibit ADP-, epinephrine- and collagen-induced platelet aggregation. The possible in vivo implications of these data are discussed.

Cell Aggregation↗
PubMedDate not supplied

[Granulocytes and oxidative metabolism: role of monoclonal antibodies in superoxide anion release from neutrophils].

We studied the effects on the granulocyte oxidative metabolism of some monoclonal antibodies reacting with a membrane surface antigen fundamental for the functions of these cells. The aim of this investigation was to evaluate the role of CD11b/CD18 membrane glycoproteins on superoxide release from granulocytes. The preincubation with MoAbs 60.1, OKM1, 60.3 determined a significant decrease of anion release from granulocytes stimulated by fMLP.

Antibodies, Monoclonal↗
PubMedDate not supplied

[Anatomopathologic and clinical correlations of squamous carcinoma of the larynx and the hypopharynx].

The most important problem in the diagnosis of neoplastic diseases is not only to identify its malignant nature but also the degree of aggressiveness since differences in prognosis and therapy exist in cancer as well. A short review of the literature regarding correlations between histologic degree of carcinoma and prognosis is presented. A total of 105 laryngectomy samples are presented taken by bilateral neck dissection following the principles employed for laryngeal or hypopharyngeal carcinoma. Reference is made to the following parameters: extension and site of primitive tumor; cellular (WHO), nuclear (Black) and structural (Carlon) gradings; histologic host response as judged by the presence and degree of lymphoid infiltration and fibrous stromal reactions around the primary tumor. Host response was related to structural grading. Regional nodes were studied for metastases. Nodal metastases were related to cellular, nuclear and structural grading. Clinical and statistical analysis proved that Carlon's grading, based on structural organization of the tumor, seen as a manifestation of its cellular cohesiveness, is a more discriminating system of malignancy than the others. The most organoid growth patterns (Structural grades 1 and 2) did not present any node metastases. They can be regarded as manifestations of "low grade malignancy tumors". The least organoid and non organoid growth patterns (Structural grades 3 and 4) showed higher levels of node metastases (Grade 3: 35.2%; Grade 4: 58%; p = 0.01). Necrosis was found only in Grades 3 and 4 and was associated with node metastases in 70%. Structural grading could be the guideline to better management of laryngeal carcinoma in terms of choice of surgical treatment (type of laryngectomy; neck dissection).

Aged↗
PubMedDate not supplied

[Breast and skin localization of non-Hodgkin's lymphoma. Description of a clinical case].

A clinical case of a patient suffering from non-Hodgkin lymphoma with breast localization and skin infiltration is reported. Skin lesion was the presenting feature: after diagnosis and chemotherapy the relapse of lymphoma was characterized by breast infiltration. These particular localizations are discussed in terms of their unfavourable prognostic significance in lymphoma.

Adult↗
PubMedDate not supplied

[Hypokalemic rhabdomyolysis and thiazide diuretics. 3 clinical cases].

Rhabdomyolysis is an acute skeletal muscle disorder characterized by altered integrity of the cell membranes of muscle fiber cells and can be caused by a variety of factors whose nature is not necessarily traumatic. Hypokalemia is one of the most common metabolic causes, accounting for about 14-28% of rhabdomyolytic syndromes. We report and discuss three cases of hypokalemia caused by diuretic treatment with hydrochlorothiazide: the main clinical features were muscle symptoms following massive rhabdomyolysis.

Adult↗
PubMedDate not supplied

Leukocyte CD11/CD18 integrins: biological and clinical relevance.

The integrin family consists of a series of related alpha beta heterodimers (subunits of 95,000-200,000 Mw) involved in a variety of cell-matrix and cell-cell adhesion functions. Leukocyte adhesion has biological importance in numerous processes involving host defense. The CD11/CD18 integrins are differentiated antigens which play a critical role in this mechanism. CD11a/CD18 are apparent on early progenitors of all myeloid and erythroid cells. CD11b/CD18 and CD11c/CD18 are more restricted antigens normally expressed on monocytes, macrophages, PMN and natural killer cells. Activated granulocytes and monocytes express far more CD11b/CD18 than the other two antigens: 6 to 8 x 10(5) CD11b/CD18 molecules appear on maximally activated granulocytes. These integrins and in particular the beta 2 subunit are lacking in a genetic disease. On the other hand, they are fundamental in numerous physiological processes and in various hematological and cardiovascular diseases. The biochemical characterization and behavior of the CD11/CD18 complex in various clinical conditions are the subject of this review.

Adult↗
PubMedDate not supplied

[The effect of opioid peptides on peripheral blood granulocytes].

Some neuropeptides may influence various functions of immunocompetent cells. Endogenous opioids have been implicated in the correlation of the immune, endocrine and nervous system. The object of our study was to evaluate the effects induced by some agonist and antagonist endogenous peptides to opioid receptors on granulocytes (PMNs) functions in vitro. We used these drugs: D-Ala2-D-Leu6-enkephalin (DADL-Sigma-Chemical-Usa); D-Ala2-N-ME-Phe4-Gly-ol5-enkephalin (DAGO-Sigma-Chemical-Usa); Dynorphin 1-9 (Sigma Chemical-Usa); naloxone (Sigma Chemical-Usa) and morphine (Sifac-Italy). Morphine was able to inhibiting neutrophil granulocyte chemotaxis induced by serum activated with E. Coli (p < 0.01), by casein (p < 0.01 and <fMLP (p < 0.01). Furthermore morphine and 1-9 dynorphin inhibited spontaneous migration of PMNs both when added to the upper compartment together with cells. Naloxone was able to inhibit PMNs chemotaxis induced by serum, casein and fMLP, this drug was not able to behave on chemokinesis or random migration activity. Morphine was found to inhibit PMNs aggregation induced by PHA (0.01), PMA (0.01), fMLP (0.01) and Ca++ ionophore (p < 0.01), ATP release (p < 0.01), thromboxane B2 (TxB2) and leukotriene B4 (LTB4) secretion during cell aggregation. Dynorphin 1-9, per sé, induced TxB2 and LTB4 secretion from PMNs. Morphine inhibition of both cell aggregation and ATP release, but not arachidonic acid metabolism product secretion, was prevented by naloxone. The naloxone sensitive impairment by morphine of CD 11/CD 18 complex surface expression observed could play a role in opioid inhibition of PMNs activation.

Adult↗