Search PubMed⌕ Search

Biomedical subjects

A M Thompson

Publications and source records attributed to A M Thompson.

At least 145 records · Page 8Linked to original sources
PubMed1986

Adam--a severely-deprived Colombian orphan: a case report.

The case study of Adam, a severely-deprived Colombian orphan, is presented. At 16 months of age the child was suffering from severe marasmic malnutrition, his development was about the level of a 3-month old, he had been physically neglected and was emotionally withdrawn. Adam was adopted at 34 months of age. At 8 years of age his Full-Scale WISC-R-IQ was 113 and at 10 years of age he scored 100% on the Michigan Assessment Test. Now at 13 years 10 months he is functioning at the 8th grade school level, which is appropriate for his age, and his weight and height are in the 50th centile range.

Adoption↗
PubMed1985

Isolation and quantitation of serum IgE levels by high-performance immunoaffinity chromatography.

High-performance immunoaffinity chromatography on monoclonal antibodies coupled to protein A-coated glass beads is a method for the rapid isolation and quantitation of immunoglobulin E (IgE) from the serum of both adult and pediatric patients. The technique is as sensitive as most immunoassays but takes less than an hour to perform. In addition to measuring total IgE in both plasma and serum, the technique provides biologically active, affinity-isolated IgE, which can be used for other clinical and research studies.

Animals↗
PubMed1985

Protein A-coated glass beads. Universal support medium for high-performance immunoaffinity chromatography.

High-performance immunoaffinity chromatography (HPIC) is a technique for the fast isolation and quantitation of both antibodies and antigens. Protein A-coated glass beads provide a stable general immobilization support for most immunoglobulin G (IgG) antibodies. In conjunction with the modern expanding repertoire of monoclonal antibodies, HPIC can be applied to the quantitation and isolation of any biological material, in an active form.

Binding Sites, Antibody↗
PubMed1985

Anti-peptide antibodies detect oncogene-related proteins in urine.

Antisera to a number of synthetic peptides predicted from nucleic acid sequences of oncogenes have been used to screen 483 urine samples of cancer patients, pregnant women, and normal controls for the presence of immunologically related proteins. Increased levels of oncogene-related proteins are found during neoplasia and pregnancy. The differential detection of these oncogene-related proteins indicates that panels of monoclonal antibodies may provide a convenient noninvasive means of detecting, classifying, and staging a wide variety of malignancies and may be useful in following fetal development during pregnancy.

Antibodies, Monoclonal↗
PubMed1985

Thymic functions in uremic rats: evidence for thymosin alpha 1 deficiency.

Changes in the circulating lymphocyte populations and thymus glands were studied in rats with experimental chronic renal insufficiency (CRI). Compared to normal or sham-operated animals, rats with CRI had significant reduction in the percentages of circulating T and B lymphocytes. CRI was also associated with marked thymic atrophy and reduction in the numbers of small cortical and medullary thymic lymphocytes. Quantitative microfluorometry revealed a significant reduction in the intrathymic concentration of a potent immunomodulator, thymosin alpha 1 in all uremic animals. There was a significant positive correlation between the percentages of circulating T lymphocytes and the intrathymic concentrations of thymosin alpha 1.

Animals↗
PubMed1985

The role of ambulatory blood pressure monitoring in the evaluation of adolescent hypertension.

We performed 24-hour ambulatory blood pressure monitoring in a group of adolescents with hypertension induced by dynamic exercise and a group of normotensive controls. Ambulatory blood pressure monitoring was performed using a Del-Mar Avionics PIII recorder with readings taken every 7 1/2 minutes. There was little inter-individual variation in mean hourly blood pressure and the difference between mean hourly readings was not significant in hypertensives. Mean 24-hour ambulatory blood pressure was 130.2/80.7 mmHg for hypertensives and 115.2/70.8 in normotensives. Mean 24-hour variability was 18.3/12.9 mmHg in hypertensives and 14.6/11.5 in normotensives. There was no significant change in blood pressure or variability between day-time and evening for hypertensives. Normotensives showed a significant reduction in both BP and variability compared to hypertensives. In adolescents with systolic hypertension, ambulatory blood pressure monitoring confirmed a failure to reduce blood pressure following routine activity. This technique maybe used as a predictive marker of early, adult essential hypertension.

Adolescent↗
PubMed1984

High-performance affinity chromatography: a rapid technique for the isolation and quantitation of IgG from cerebral spinal fluid.

Standard techniques for the quantitative measurement of IgG in cerebral spinal fluid take up to 24 hs. This often delays diagnosis and treatment, critical in newborn infants. A high-performance affinity chromatography (HPAC) column, containing immobilized anti-IgG antibody, produced the same or better results in 1 h. The HPAC system gave a 98% correlation with the standard techniques at the normal-abnormal IgG level, but was more accurate at the extremely low IgG level.

Cephalometry↗
PubMed1984

Glomerulocystic kidney disease. Report of an adult case.

A case of glomerulocystic kidney disease (GCK) in an adult is reported. The patient described here had no other congenital malformations, and there was no family history of renal disease. The histopathologic findings support the glomerular origin of GCK. This case illustrated that GCK may be associated with stable renal function for many years. In view of this, GCK should be considered in the differential diagnosis of cystic kidney disease in the adult.

Adult↗
PubMed1983

The antinociceptive activity of meptazinol depends on both opiate and cholinergic mechanisms.

1 Antinociceptive responses to meptazinol, morphine and oxotremorine and the effects of pretreatment with naloxone or scopolamine on these responses in mice and rats, were examined. 2 Meptazinol evoked larger increases in nociceptive thresholds in the mouse than in the rat, whereas morphine induced large increases in both species. Oxotremorine was both a more potent and a more effective antinociceptive agent in the mouse than in the rat. 3 Antinociceptive responses to meptazinol were consistently inhibited in animals pretreated with naloxone, whereas scopolamine attenuated the effects of meptazinol in some, particularly the mouse tail immersion test, but not in all of the procedures used. 4 Naloxone inhibited all antinociceptive responses to morphine, and scopolamine inhibited all responses to oxotremorine. However, there was no significant interaction between naloxone and oxotremorine or between scopolamine and various opioid analgesic agents. 5 These results indicate that meptazinol, unlike established opioid drugs, may induce antinociception by a dual action on opiate and cholinergic mechanisms.

Analgesics↗
PubMed1983

Acute myelomonocytic leukemia associated with nephrotic syndrome. A case report with immunological studies.

A patient with acute myelomonocytic leukemia (AML) developed nephrotic syndrome. The renal biopsy showed focal glomerulosclerosis by light microscopy. Electron microscopy and immunofluorescence revealed electrondense deposits, IgG and C'3 in the glomerular mesangium. A 21S circulating immune complex (CIC) present in the patient's serum and the renal biopsy eluate contained immunochemically identical materials. The isolated antibodies from the 21S CIC and the eluate showed restricted reactivity against autologous AML cells. Immunodiffusion studies demonstrated common antigenicity between the 21S CIC antigen, the eluted antigen and between autologous AML cell membrane antigens.

Antigen-Antibody Complex↗
PubMed1983

An ultrastructural study of the marginal transitional zone in the rabbit knee joint.

The ultrastructure of the marginal transitional zone of femoral articular cartilage has been studied in the rabbit knee. There is an abrupt boundary between the convex margin of the cartilage and the synovial membrane. This is due to the arrangement and amount of collagen and of cells, because cell ultrastructure changes gradually from synovium to cartilage. The densely fibrous marginal synovium contains scattered fibrocytic cells with sparse cytoplasm and long filopodia. Near the synovium/cartilage interface, oval boundary cells containing more abundant cytoplasm abut on the cartilage matrix. In the periphery of the cartilage, an edge-belt of collagen fibrils runs obliquely from articular surface to subchondral bone. Chondrocytes near the edge-belt, whatever their depth from the articular surface, ultrastructurally resemble middle zone (zone II) cells of articular cartilage generally. The synovial surface of the marginal zone is smooth and resembles articular cartilage surfaces. Most intimal cells contain plentiful granular endoplasmic reticulum and Golgi membranes and hence are intermediate between A and B synoviocytes commonly found elsewhere. Non-fenestrated (type I) capillaries lie in a superficial stratum beneath the synovial surface and in a deep stratum near the synovium/cartilage boundary, and are surrounded by pericytes. No mast cells, macrophages, lymph vessels or nerves could be identified in the marginal zone. Contrary to earlier accounts of collagen orientation in this zone, most of the fibrils in the marginal synovium appear to run around the perimeter of the cartilage and only a few bundles run radially from the synovium towards the cartilage. It is suggested that the circumferential collagen both contains the marginal cartilage and prevents displacement of synovial tissue on to the articular surface. The radial strata of collagen serve to anchor the circumferential collagen to the cartilage edge-belt. In agreement with earlier investigators, it is considered that the edge-belt withstands tensile stresses arising from deformation of the articular surface. The role of the marginal synovium is also discussed in relation to synovial fluid formation and cartilage nutrition.

Animals↗
PubMed1980

Effect of acetazolamide on rate of CO2 uptake in the perfused rat brain.

The accumulation of acid-labile CO2 was measured in the rat brain at early times after exposure to 30% CO2 in oxygen. In particular, the effect on CO2 accumulation of inhibiting brain carbonic anhydrase with acetazolamide was studied. For 57 rats, a rat head perfusion technique was used which permits control and rapid alteration of arterial acid-base conditions. At a paCO2 of 35 mm Hg, total carbon dioxide of the whole brain remained at a normal mean value of 13.4 +/- 0.6 (S.E.) mM/kg during perfusions 209-160 min long. On 30% CO2, brain CO2 increased rapidly to a mean of 24 mM/kg at 5 min and then much more slowly. The latter curve agreed well with values obtained in intact animals. Pretreatment with acetazolamide had no inhibitory effect on CO2 accumulation at 1-5 min. At 5 min, control brain CO2 was 23.9 +/- 0.6 (n = 10) and acetazolamide-pretreated brain CO2 was 24.4 +/- 0.7 (n = 9). Terminal venous pCO2s were 164-180 and 167-172 mm Hg, respectively. Thirty-one unperfused rats were also investigated with similar results. Acetazolamide-pretreated rats had the same CO2 uptake as controls after 15 min exposure to CO2, rather than lower uptake as would be expected if carbonic anhydrase were rate-limiting. The results suggest the need for reinterpretation of some concepts about the role of carbonic anhydrase in CO2 accumulation in the brain.

Acetazolamide↗
PubMed1978

Extensive cerebral nocardiosis cured with antibiotic therapy alone. Case report.

A 34-year-old renal transplant recipient developed multiple soft tissue and extensive cerebral nocardiosis. The number and locations of the cerebral abscesses and probable areas of cerebritis precluded surgical drainage procedures. Gradual resolution of all the cerebral lesions occurred over a period of 6 months with antibiotic therapy alone. A review of the literature revealed only one previous case of a patient with pulmonary nocardiosis who had a probable secondary brain abscess and who survived without surgical drainage. Thus, if surgical drainage is not possible, antibiotic therapy alone may offer a hope of survival in what otherwise has been considered a uniformly fatal disease.

Adult↗