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Biomedical subjects

A M Duprat

Publications and source records attributed to A M Duprat.

At least 73 records · Page 4Linked to original sources
PubMed1981

A rapid experimental method to study primary embryonic induction.

The technique described here is a combination of the sandwich-method and cell culture. It allows one to know quickly whether induction occurred or not (48 h if cell spreading is considered, 4-5 days if morphological differentiation is observed). The dissociation of the explants soon after induction does not disturb their inductive pattern. Moreover, this method allows a daily observation of the morphological events and study at the cellular and/or molecular level.

Animals↗
PubMed1976

In vitro culture of larval amphibian erythroblasts.

A larval erythroblast culture method is described. By this method, it is possible to cultivate for several weeks a homogeneous population of cells (5-10(5) cells/ml medium on average after 4 or 5 days of culture), which are relatively synchronous with regard to their state of differentiation.

Animals↗
PubMed1975

Immunofluorescence studies on amphibian myoblast differentiation.

Differentiating myoblasts from urodele amphibians were cultivated in vitro and treated by immunofluorescence techniques and (or) inhibitors of RNA and protein syntheses. These experiments lead to the conclusion that the differentiation of these cells is directed by long-lived m-RNAs which are already present in morphologically undifferentiated cells and may act for at least 6 days. Contrary to observations on cultivated myogenic cells from other groups, the fusion of urodele myoblasts is not a prerequisite for a normal and functional differentiation (contractions) since isolated myoblasts differentiate quite normally. The appearance and further evolution of myosin and actin occur synchronously in this biological system.

Actins↗
PubMed1975

[Biological effects of solutions of non-histone chromatin-associated proteins on cell differentiation in vitro (author's transl)].

Nuclear non-histone (NNH) proteins, both chromatin-associated and otherwise, have been prepared in defined conditions, either from nuclei or from washed chromatin. When these NNH proteins are homospecific, they produce inhibitory effects on morphological differentiation of embryonic Urodelan Amphibian cells cultivated in vitro. When they are heterospecific, on the contrary, they have no action on the differentiation of those cells. It is concluded that the effects of the non-histone proteins on cell differentiation depend upon the relationship between the animal species which is used to provide the proteins and that used to provide the reactive material.

Ambystoma↗