Cerebrospinal fluid protein electrophoresis without prior concentration.
There is a need for new methods to study CSF proteins. Kerenyi et al., 1973 and Verheecke, 1975 have already used this method and discussed its advantages. The electrophoresis is carried out according to the method of Wieme with as supporting medium agar. The volume applied varies between 5 to 15 microliter according to the total protein content and the width of the slit. Care must be taken to work at all times with bidistilled water and pro analysis reagents. After drying, the electrophoretic plates are reduced in a 10% solution of thiodiglycol, dried again, then immersed in 2% potassium hexacyanoferrate, washed thoroughly, then revealed with a silver nitrate solution (Kerenyi et al., 1973; Verheecke P., 1975) and left in 1% acetic acid. This technique is of major value as: 1. It avoids artefacts due to concentration and loss of proteins; 2. It works with very small amounts of fluid; 3. Where the CSF is silent for antibodies restricted heterogeneity using the classical methods, it reveals marked IgG Fractionation.