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Biomedical subjects

A Lindner

Publications and source records attributed to A Lindner.

At least 145 records · Page 8Linked to original sources
PubMed1992

[The effect of storage time and temperature on the activity of glutathione peroxidase in plasma and whole blood of horses].

The influence of temperature and duration of storage on glutathione peroxidase (GSH-Px) activity in plasma and whole blood samples of horses was investigated. Furthermore the relationships between the GSH-Px activities in plasma and whole blood as well as the GSH-Px values related to hematocrit and the hemoglobin content of the blood samples of 93 different horses were calculated. At 20-22 degrees C, the GSH-Px activity in plasma and whole blood samples remained stable over at least three days while it was reduced by 38% and 65% after 2 and 3 days, if the whole blood samples were kept stored after being diluted (lysate). The GSH-Px activity was stable over three days in plasma, whole blood and lysate samples stored at 4 degrees C and -18 degrees C. In lysate samples kept frozen the GSH-Px activity remained stable over a period of 60 days while in plasma samples the activity had fluctuations. The GSH-Px activity in plasma was 12.2 +/- 11.7 nkat/ml, in whole blood 386 +/- 117 nkat/ml. The coefficient of correlation between plasma and whole blood GSH-Px activity was r = 0.27 (p less than 0.01), and between plasma and whole blood related to the hematocrit and to the hemoglobin content of the blood samples 0.38 and 0.35 respectively (both p less than 0.001). Between whole blood and whole blood related to hematocrit and hemoglobin content, r was 0.8 and 0.89 respectively (p less than 0.001).

Animals↗
PubMed1992

[High-dose-rate brachytherapy of prostatic carcinoma with iridium 192].

In the therapy of localized prostatic cancer the radical prostatectomy shows good results within a five-year interval with no evidence of disease in nearly 90%. An important alternative is the radiotherapy by external beam or interstitial technique with iodine, gold or iridium. We use the high dose rate technique with Ir-192. In this technique five to seven hollow needles are placed in the prostate from perineal punctures under transrectal sonographic control. The three-dimensional brachytherapy planning is done according the actual needle position. A computer program calculates the radiation dose and distribution for each needle by adjustment of time and stops of the Ir-implants. The Ir-192 is temporary loaded twice with 9 Gy supplemented with external beam radiation (18 x 2 Gy). Since 1985 29 patients with localized tumor (T1-T3, N0, M0) have been irradiated. 21/29 had a pelvic lymphadenectomy before. In 85% of the patients were seen no side effects. Only one patient had a serious complication through a recto-vesical fistula. Out of the 21 followed-up patients 16 were in full remission, three had an androgen deprivation because of progression. A local tumor control could be demonstrated by cytology in 70% of the patients. This rational technique seems to be an alternative for patients not eligible for a radical prostatectomy.

Aged↗
PubMed1991

Effects of prostatectomy on sexual function.

We evaluated the effects of prostatectomy on sexual function in 210 patients, 49.6 percent of whom underwent transurethral resection of the prostate (TURP), and the remainder had suprapubic transvesical prostatectomy (SPP). Pre- and postoperative interviews with detailed questionnaires were utilized. Postoperative sexual dysfunction was reported by 18 of 152 patients (11.8%) who were functioning normally prior to surgery. The incidence of postoperative impotence was evenly distributed between the TURP and SPP groups and was age-related; it was highest among older patients. We further observed a strong correlation between the presence of a permanent sexual partner and the preservation of potency. We conclude that the risk of postoperative impotence is dependent on both the patient's age and the presence of a partner, and should be discussed with the patient preoperatively.

Aged↗
PubMed1991

On the morphology of the terminal microvasculature during endochondral ossification in rats.

The investigation was carried out on the proximal tibia metaphysis by means of India ink-injection, reconstruction, corrosion casting combined with scanning electron microscopy, and transmission electron microscopy of thin sections. The terminal vasculature during endochondral ossification proved to be a labyrinth of interconnected sinusoidal complexes with bulbous buds invading the chondrocyte cavities of the growth plate. This system of vessels was canalized throughout. A special feature was the existence of endothelial pockets. The formation of the terminal microvasculature as a labyrinthine cavity during endochondral ossification, together with the still incompletely developed bone could be a weak point for compression injuries.

Animals↗
PubMed1991

Measurement of intra-oral negative air pressure during dummy sucking in human newborn.

Non-nutritive sucking on a dummy was studied in 23 clinically normal full-term infants with a mean gestational age of 40 weeks (SD 1.5). The intra-oral negative air pressure was measured by a miniature air pressure transducer inside a commercial dummy. The sucking pattern in these infants was always composed of periodical changes between sucking burst and interburst periods with a mean rate of 5.3 (SD 1.6) bursts per minute. The mean rate of sucks within a sucking burst was 1.7 (SD 0.2) sucks/sec. The mean value of maximum intra-oral negative air pressure among newborn was 58 mbar (SD 14.6) during dummy sucking. During interburst periods the negative air pressure remained at a mean value of 8.4 mbar (SD 5.8).

Child, Preschool↗
PubMed1991

Cheek and lip pressure against maxillary dental arch during dummy sucking.

The sucking pattern and intra-oral negative air pressure were studied simultaneously with the cheek/lip pressure in 12 children when sucking a dummy. The cheek/lip pressure was measured at the canine and at the second primary molar on the right side of the upper dental arch. The cheek/lip pressure peaks followed the intra-oral negative air sucking pressure peaks continuously. In the canine region the mean value of maximum cheek/lip pressure was 54 g/cm2. The corresponding value at the second primary molar was 21 g/cm2. During dummy sucking the mean cheek/lip pressure was 3.4 times higher against the canine compared to the second molar (P less than 0.001). The intra-oral negative air pressure was approximately the same whether the cheek/lip pressure was measured at the canine or at the second primary molar. The results indicated that the circumoral muscles were especially active during dummy sucking, in addition to the cheek pressure in the canine region.

Cheek↗
PubMed1991

Histopathology of benign prostatic hyperplasia after failure of hyperthermia treatment.

We have used a fine morphometric method to evaluate hyperplastic prostate tissue after treatment by local hyperthermia and compare it with untreated specimens. Local hyperthermia was delivered to the prostate gland by a transrectal applicator using microwaves at 915 MHz. Prostatic tissue was obtained from 13 patients who had completed a course of local hyperthermia treatment and who underwent prostatectomy 3 to 12 months later because the treatment had failed. Prostatic tissue from 9 patients who had undergone prostatectomy with no previous treatment served as a control. A significant reduction in the volume fraction of fibrous tissue was found in the study group (37%) compared with the general population (48%). No inter-group difference was observed in the volume fractions of vascular or glandular tissue. We suggest that the difference observed in the fibrous elements may be the reason for the failure of treatment and that the histological composition of the diseased gland may, therefore, be a key factor in determining the outcome.

Aged↗
PubMed1991

Serum prolactin levels in patients treated with a gonadotropin-releasing hormone analogue for adenocarcinoma of the prostate.

Twenty-six patients with advanced cancer of the prostate were treated with [D-SER(BUt)6]LHRH-(1-9) nonapeptide ethylamide (buserelin). All but 6 patients evidenced signs of metastasis. Blood prolactin levels were measured prior to the initiation of treatment and during a follow-up period of 18 months. A statistically significant (p greater than 0.0005) elevation in serum prolactin levels was observed after 3 months of treatment. Serum prolactin remained elevated for a total of 6 months, after which a decline to pretreatment levels was observed. Our observation of a transient rise in prolactin levels during the chronic administration of buserelin is at variance with previously published data which reported unchanged prolactin levels during such treatment.

Adenocarcinoma↗
PubMed1991

[Surgical treatment of urinary stress incontinence in women].

32 women with genuine stress urinary incontinence were operated on via the transvaginal approach during 1989-1990. They ranged in age from 27-72 years, with a mean of 55.7. In 26, in addition to the urine leakage, vaginal wall prolapse of various degrees was diagnosed. 6 underwent Raz modified Pereyra bladder neck suspension, 26 had Raz 4-corner bladder and bladder neck suspension, and in 17 rectocele and perineal repair were also performed. 2 with uterine prolapse also underwent vaginal hysterectomy. At follow-up 2-12 months later 29 of the 32 were completely cured of stress incontinence (90.6%). In 7 urge incontinence persisted in various degrees, and 3 had developed urge incontinence de novo. All but 1 of them responded favorably to anticholinergic medication. There were postoperative complications in 5 (15.6%), including wound infection that required removal of Prolene sutures in 2, urethrovaginal fistula requiring reoperation in 1, perioperative myocardial infarction in 1, and in 1 voiding dysfunction initially treated with self-intermittent catheterization, but later cured by removal of a pair of Prolene sutures.

Adult↗
PubMed1991

Comparison of clinical chemical variables in blood plasma and serum of horses.

Several clinical chemical blood variables were compared, in order to evaluate the differences between Na heparinized plasma and serum samples. Samples from 45 healthy horses were used. No differences between the two sample substrates were found for aspartate aminotransferase, lactate dehydrogenase, lactate dehydrogenase-isoenzymes, creatine kinase, alkaline phosphatase, bilirubin, cholesterol, urea, total protein, alpha-globulin, gamma-globulin, albumin, calcium (Ca), phosphate (P), sodium (Na) and potassium (K). gamma-Glutamyltransferase and beta-globulin were significantly higher in heparinized plasma than in serum (each p less than 0.05) while magnesium (Mg) was lower (p less than 0.05). From the horse group used for the study, thoroughbreds in racing condition had significantly higher aspartate aminotransferase, gamma-glutamyltransferase, alkaline phosphatase, bilirubin, P and Mg as well as lower Ca and K values than riding horses, irrespective of the sample substrate used. It was concluded that expect for gamma-glutamyltransferase, beta-globulin and Mg, there was no significant difference between the clinical chemical variables of Na heparinized plasma and serum samples.

Animals↗
PubMed1991

[LDH and CK isoenzyme patterns in the blood plasma of horses with elevated CK, LDH and AST activities].

The distribution of LDH and CK isoenzymes in blood plasma of ten clinically sound Thoroughbreds with reasonable performance and without elevated clinico-chemical blood variables (reference group) was compared with 57 Thoroughbreds, which had histories of mild locomotor disturbances and/or poor performance and had elevated CK, LDH and/or AST activities (trial group). The trial group was subdivided according to the number of altered blood variables and in the groups with two as well as three altered blood variables also according to the extent of alteration of the total CK activity. The pattern of LDH and CK isoenzyme distribution in the blood plasma of the reference group was the following: 22% LDH1, 36% LDH2, 34% LDH3, 6% LDH4 and 2% LDH5 as well as 75% CK1 and 15% CK2. The remaining 10% of the plasma electropherogram could not be alloted to any one of the two CK bands. All trial groups built showed a similar pattern of changes in their isoenzyme distribution independent on kind and combination of altered enzyme activities. The shares of CK1, LDH4 and LDH5 were significantly increased whilst the shares of CK2, LDH1 and LDH2 decreased. A multiple analysis of variance demonstrated that only increased total CK activities had a pronounced effect on distribution of LDH and CK isoenzyme patterns in the trial group (p less than 0.01 for LDH2, LDH3, LDH4, CK1 and p less than 0.05 for CK2). The conclusion of the study was that the altered distribution pattern of LDH and CK isoenzymes of the trial group signalized an increased skeletal muscle membrane leakage.

Animals↗
PubMed1991

[The effect of storage time, temperature and initial test material on clinico-chemical blood variables].

The effect of temperature and duration of storage on clinico-chemical variables was investigated in Na heparinized plasma and plasma or serum of Na heparinized whole blood and whole blood of horses, respectively. The values of AST, Gamma-GT, AP, bilirubin, cholesterol, urea, total protein, albumin, Ca, Mg and Na varied in all the sample substrates investigated at 20-22 degrees C and 4 degrees C less than +/- 10% during the observation period of up to four days. In the Na heparinized plasma samples kept at 20-22 degrees C and 4 degrees C also LDH, CK, inorganic P and K did not differ significantly from its initial values during the four days. However, in the plasma of sampled stored at 20-22 degrees C as Na heparinized whole blood, the increases of LDH, CK, P and K until the fourth day of storage reached 56%, 49% and 69% and in the serum of samples kept as whole blood 90%, 165%, 147% and 48% respectively. While at 4 degrees C in the plasma of samples stored as Na heparinized whole blood until the fourth storage day only K increased significantly (by 28%), the LDH, CK and K values in the serum of samples stored as whole blood rose to 118%, 136% and 141% respectively. In the Na heparinized plasma samples kept at -18 degrees C all the measured variables did not differ significantly from the initial values during the storage time of 10 weeks.

Animals↗
PubMed1990

[Reference values for cortisol, T4 and T-uptake in different horse groups using the fluorescence polarization immunoassay (FPIA)].

Reference values for Cortisol, T4 and T-Uptake, determined with the Fluorescence-Polarization-Immunoassays (FPIAs) in blood-plasma of different horse groups were established. The highest Cortisol values were measured in blood samples from thoroughbred racehorses and riding horses taken between 7 and 8 a.m. (181 +/- 37 and 268 +/- 43 nmol/l), the lowest gained between 5 and 6 p.m. (69 +/- 45 and 85 +/- 32 nmol/l respectively). Peak values for T4 in riding horses were found in blood samples collected between 1 and 2 p.m. (28.2 +/- 5.7 nmol/l) followed by the samples taken at 5-6 p.m. and 7-8 a.m. T-Uptake values did not show a diurnal variation. Values of thoroughbred racehorses were much lower than in thoroughbred broodmares and riding horses (0.11 +/- 0.03, 0.18 +/- 0.03 and 0.24 +/- 0.05 units). The blood samples collected on 5 successive days showed significant variations for Cortisol. T4-values were stable whilst T-Uptake had small variations.

Animals↗
PubMed1990

Carcinoid tumor of skin: report of a possible primary case.

A case of a possible primary carcinoid tumor of the skin in a 40-year-old man is presented. The neoplasm was diagnosed as consistent with carcinoid tumor on the basis of conventional light microscopy, immunohistochemical studies, and electron microscopy. Workup revealed no evidence of carcinoid tumor elsewhere. Metastases to the skin from internal carcinoid tumors are uncommon, but presumed primary carcinoid tumors that arise in the skin are extremely rare; only three cases have been found in the English-language literature.

Adult↗
PubMed1990

Serum prostate specific antigen levels during hyperthermia treatment of benign prostatic hyperplasia.

To understand better the tissue and cell changes occurring in the human prostate as a reaction to heat, we used circulating prostate specific antigen as a marker to detect cell injury. The prostate in 18 patients with benign prostatic hyperplasia was heated to 42 +/- 1.5C with local microwaves at 915 MHz. Each treatment sessions lasted 1 hour and the patients underwent 5 treatment sessions. A total of 90 treatment sessions was performed among 18 patients. No significant difference was observed between pre-treatment and post-treatment serum prostate specific antigen levels. We conclude that local hyperthermia is an atraumatic treatment modality that does not exert its effect on the hyperplastic prostate via a cytotoxic insult to prostate epithelial tissues.

Antigens, Neoplasm↗