Biomedical subjects

A Lindgren

Publications and source records attributed to A Lindgren.

208 records · Page 12Linked to original sources

PubMedDate not supplied

Menopausal status and cut off levels of steroid receptor ligand binding assays in breast cancer.

494 tubuloductal breast carcinomas obtained at operation were assayed for ER and PgR with short-term ligand incubation and isoelectric focusing. Plasma FSH and E2 concentrations available from 156 of the patients showed strictly premenopausal endocrine conditions in patients 45 years or younger; strictly postmenopausal conditions were found at 55 years or older. ER concentrations were significantly lower in biopsies from premenopausal compared with those from postmenopausal patients. ER concentrations assayed in intervening perimenopausal age period were not statistically different from the premenopausal period. An arbitrarily chosen cut off level to differentiate receptor low from receptor high tumours divided premenopausal assays into two equal parts; those from postmenopausal patients were cut at the second lower percentile. Arbitrary cut off levels ignorant of menopausal status should be replaced by fractionation of low and high receptor tumours on a percentile or quartile basis. Clinically, subgroups or subpopulations of patients should be identified with regard to endocrine and/or receptor status and evaluated separately.

Adult↗

PubMedDate not supplied

Differences in DNA content and proliferation rate between adenocarcinomas of the uterine cervix and corpus.

The DNA content and the S-phase rate of 26 cervical adenocarcinomas and 100 endometrial adenocarcinomas, determined by flow cytometry, were compared. Statistically significant differences in DNA patterns were found, although only pure adenocarcinomas were studied in both groups. Peri-diploid values (1.8c-2.2c) were less common in cervical adenocarcinomas (38%) than in endometrial carcinomas (65%) (p less than 0.05). Six (23%) of the cervical carcinomas and 8 (8%) of the endometrial carcinomas had two detectable stem lines. The mean S-phase rate was significantly higher in adenocarcinomas of the uterine cervix than in endometrial carcinomas (p less than 0.02). The dominance of aneuploid values, the high proportion of polyclonal tumors and the high S-phase rates of the cervical adenocarcinomas have more similarities with the DNA patterns of cervical squamous cell carcinomas than with those of endometrial adenocarcinomas.

Adenocarcinoma↗

PubMedDate not supplied

Blood group isoantigen expression during tumour progression of cervical neoplasia.

Twenty-nine cases of microinvasive squamous and 38 cases of progression from preinvasive to invasive carcinoma of the cervix were immunostained with monoclonal antibodies against blood group isoantigens for expression in normal, dysplastic and neoplastic epithelium. The results show a varying staining pattern without correlation to tumour progression, recurrence or survival.

ABO Blood-Group System↗

PubMedDate not supplied

Accuracy of blind cytologic diagnosis of diploid breast cancer.

Fifteen fibroadenomas and 20 adenocarcinomas with identical diploid DNA histogram patterns were blindly diagnosed in fine needle aspirates by 22 cytopathologists. Despite the total lack of clinical information, each cytopathologist, on the average, managed to correctly diagnose 33 of the 35 cases. There were more false diagnoses among the fibroadenomas than among the cancers.

Adenocarcinoma↗

PubMedDate not supplied

Blood group antigens in relation to DNA content, S-phase rate and heterogeneity, and their prognostic values in cervical carcinoma.

Evaluations were made of the expression of blood group antigens, DNA content and S-phase rate in a prospective study of 97 patients with squamous cell carcinoma of the uterine cervix. No correlation was found between loss of ABH expression and ploidy level or the degree of proliferation, whereas correlations were found to age and stage. In 11 (42%) of 26 tumors with two or three detectable stemlines, distinct areas with disparate ABH staining patterns were found, indicating genotypic and phenotypic heterogeneity within these tumors. In tumors with one stemline significantly fewer such tumors were found (p less than 0.01). Twenty-four early recurrences (within 20-36 months) after treatment were found. High S-phase rates (greater than 20%) were associated with a significantly increased recurrence rate (p less than 0.05). Loss of ABH antigen expression or ploidy level were not statistically correlated to early recurrences.

ABO Blood-Group System↗

PubMedDate not supplied

Blood group isoantigen and involucrin expression in cervical intraepithelial neoplasia.

258 patients with dysplastic cervical lesions reclassified as CIN 1-3 and AIM 1-3 and with up to ten years of follow-up were immunostained with monoclonal antibodies against blood group isoantigens and involucrin for expression in normal and dysplastic squamous epithelium. The results show a varied staining pattern with a majority of unchanged staining properties in normal and dysplastic epithelium, the same frequency of increase as decrease in dysplasia and no correlation to progression or recurrence, in variance with previous reports.

ABO Blood-Group System↗

PubMedDate not supplied

Increased recovery of estrogen receptor in crude homogenates of breast cancer biopsies. A preliminary report.

Crude homogenates and cytosols of 31 breast cancer biopsies were analyzed for estrogen receptor, using the ER-EIA monoclonal assay (ABBOTT Laboratories). The recovery of the receptor was significantly higher in all homogenates than in their corresponding cytosols, in six of which the receptor was below the level of detection. The range of the ratio between homogenate and cytosol concentrations was 0.9-8.1 for 25 positive biopsies. If the recent hypothesis of nuclear localization of the receptor is accepted, the values obtained with crude homogenates would more correctly reflect the true concentration of the receptor in the cells.

Biopsy↗

PubMedDate not supplied

Variation of pH of mammary tumour cytosols: potential influence on steroid receptor assay.

Mammary tumour cytosols prepared for steroid receptor assay showed pH values above or below 7.3-7.5 in 50% of the samples. An increase of 0.6 pH units was observed after the addition of dextran coated charcoal. To test the sensitivity of the assay systems to pH changes, breast cancer cytosols were adjusted to pH between 6.0-9.0 before incubation with ligand at 0 degrees C. ER was then separated by isoelectric focusing in a pH gradient between 9.5-3.5 in a routine assay including a dextran coated charcoal step before focusing. PgR was assayed by electrofocusing as well as by a conventional DCC method and Scatchard plot. Unadjusted control cytosols were run in parallel. At pH above or below 7.0-8.0 recovery of receptor was reduced, the loss being most prominent at low pH. At low pH levels Scatchard plots appeared to be of curvo-linear shape. The effect of low intracellular tumour pH on receptor-ligand affinity in vitro may be of great importance as well in vivo, and should be taken into consideration in the therapeutic situation.

Breast Neoplasms↗

PubMedDate not supplied

Fine needle aspiration cytology of tubular breast carcinoma and radial scar.

Fine needle aspiration cytology (FNAC) findings in 33 tubular breast carcinomas (TC) and 10 radial scars (RS) were reviewed. In 19 (57%) cases of TC, a categorical diagnosis of malignancy could be made. Four (12%) TC, all containing myoepithelial cells, were misdiagnosed as benign. None of the RS was misinterpreted as malignant on FNAC. All RS smears contained myoepithelial cells. Although RS shared some of the cytologic features of TC, the lack of conspicuous nucleoli and pleomorphism prevented a false-positive diagnosis. Tubular angular structures, considered to be a characteristic feature of TC, may also occur in RS. Cellularity was poorer in RS than TC. The FNA yield obtained when attempting to aspirate small TC and RS lesions may originate in adjacent breast lesions and give a misleading cytologic picture. The occurrence of myoepithelial cells and/or lack of dissociation are not unequivocal benign signs in FNA smears; such findings do occur in TC. Finally, the difficulties that may be encountered in the diagnosis of TC and RS by frozen section are briefly discussed. Radiology often helped in establishing a diagnosis of malignancy in TC but did not help in ruling out malignancy in RS. A teamwork approach to the diagnosis and management of these lesions is recommended.

Adenocarcinoma↗

PubMedDate not supplied

Endometrial carcinoma: the prognostic impact of papillary serous carcinoma (UPSC) in relation to nuclear grade, DNA ploidy and p53 expression.

In a histopathological review of 266 women with stage I-IV endometrial carcinoma, the prognostic significance of uterine papillary serous carcinoma (UPSC) and clear cell carcinoma (CCC) of the endometrium was compared with that of ordinary adenocarcinoma and adenoacanthoma. UPSC and CCC were diagnosed in 31/266 (12%) and 6/266 (2%) of the patients respectively. The median follow-up time was 79 months (range 3 to 133 months). 49% of the patients with UPSC or CCC died of their cancer compared with 31% in the adenocarcinoma + adenoacanthoma group (RH=2.25; p=0.0022). Strong expression of the mutated p53 gene product was significantly more common in the UPSC + CCC group than in the reference group (64% vs. 19%), (p<0.0001). In a Cox multivariate analysis including histopathological and clinical variables, UPSC and CCC were still significant predictors of survival (p<0.05). When nuclear grade was added to the analysis, UPSC + CCC as well as the degree of differentiation lost most of their prognostic impact. In a final multivariate analysis, DNA ploidy was found to be the strongest predictor of outcome besides age and clinical stage.

Adenocarcinoma↗