Biomedical subjects
Publications and source records attributed to A Lindgren.
A case with characteristic roentgenographic and microscopic findings of adamantinoma tibiae is reported. Knee disarticulation with early ambulation is the treatment of choice and was performed on this patient. Limb ablation followed by Unna Paste Bandage to prevent oedema and permit early prosthetic fitting facilitates rehabilitation. The 1 year follow-up showed excellent prosthetic function and no evidence of residual tumour.
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Two immunoglobulin-producing lines of normal lymphoblastoid cells have been established from cultures of two human astrycytomata. These had the same characteristics as lymphoblastoid lines derived from normal human lymphoid tissue or peripheral blood. It is concluded that the lymphoblastoid lines are derivates of non-neoplastic lymphoid cells (perhaps of the B series), which were present as an infiltrate within the astrocytomata. Furthermore, the slow emergence of the lymphoblastoid cells in culture, their continuing requirement for feeder cells and their production of monoclonal rather that heteroclonal immunoglobulin are attributed to an artefact of growth conditions.
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In 1244 women with breast symptoms an evaluation by means of palpation was made with respect to diagnosis of malignancy according to a four-grade scale ranging from "definite cancer" to "no cancer." Aspiration biopsy and cytologic examination were then performed in 984 breast lesions. The diagnosis from the cytologic evaluation ranged from benign, through three grades of atypia (slight, moderate, grave) to cancer. A histologic diagnosis was made in 411 cases and in 28% it was cancer. Cancer was found in 92.5% of the patients with a palpatory diagnosis of "definite cancer," and in 50% of those with a palpatory diagnosis of "strong suspicion of cancer." In all patients in whom cancer was diagnosed cytologically, the same diagnosis was made at histology, while 87.5% of those with grave atypia at the cytologic examination were diagnosed histologically as having cancer. A false negative cytologic diagnosis was made in 4% of the cancer cases. With a combination of palpation and cytology, 91% of the cancer cases fell within the groups "definite cancer," "strong suspicion of cancer" (palpation)/"cancer," "grave atypia" (cytology). No patients with cancer were evaluated as "no cancer" (palpation)/"no atypia" (cytology). In this group of 697 patients, however, one cancer was discovered after 7 months. The investigation showed that a thorough palpatory evaluation is a prerequisite for a good result of aspiration biopsy, in particular to meet the risk of a false negative cytologic diagnosis. The cytologic examination revealed cancer in 6 and 12 cases, respectively, when palpation gave no or some suspicion of cancer, and in many cases it was able to eliminate malignancy suspected on palpation. Cytologic atypia indicated cancer in a relatively high per cent, but was also noted in many cases found to be benign histologically. The possibilities of reducing the number of "unnecessary" surgical biopsies by using a combination of palpation and cytology is discussed.
In 117 women with breast cancer, breast size, tumour size, suspicion of malignancy and clinical stage (Columbia Clinical Classification) were assessed clinically by palpation. Tumour size, degree of differentiation, malignancy grading (Ackerman), co-existent cystic fibroadenosis and axillary node metastases were assessed histopathologically. Differentiation was also assessed cytologically. There was good agreement between clinical and pathological measurements of tumour size. Ackerman's histopathological grading of malignancy correlated with the other criteria better than some other systems of malignancy grading. A higher histopathological malignancy grade was found in larger tumours and older patients. There was no clear relationship between cytological and histopathological malignancy grading. Lymph node metastases occurred three times more often in large breasts than in small ones, in spite of relatively slight differences in tumour size. Cancers in breast with cystic fibroadenosis were smaller, less malignant histopathologically and had fewer lymph node metastases than cancers in breasts without cystic fibroadenosis. "Definite cancer" on palpation was more often associated with a highly malignant tumour than a less suspected palpatory finding.
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PURPOSE: Relative regional cerebral blood flow (rCBF) at rest was measured in 44 volunteers using both dynamic susceptibility contrast (DSC) MRI and (99m)Tc-HMPAO SPECT on the same day. METHOD: In MRI, a Gd-DTPA-BMA contrast agent bolus (0.3 mmol/kg body wt) was monitored with a simultaneous dual FLASH pulse sequence (time resolution 1.5 s). MRI-based rCBF images were calculated by singular value decomposition-based deconvolution of the measured tissue concentration-time curve with an arterial input function from a small artery within the imaging slice. In the SPECT investigation, 900 MBq of (99m)Tc-HMPAO was injected intravenously. Relative rCBF in gray matter in the thalamus and in frontal white matter was determined. RESULTS: The ratio of relative rCBF in gray matter to relative rCBF in white matter was 2.21 +/- 0.57 using MRI and 2.24 +/- 0.54 using SPECT (mean +/- SD). CONCLUSION: Relative rCBF maps from DSC MRI and (99m)Tc-HMPAO SPECT showed good agreement, and the MRI-based rCBF ratio correlated with the corresponding SPECT-based ratio (r = 0.79, p < 0.0000006).
The magnetic resonance imaging (MRI) and computed tomography findings in 28 patients with the clinical diagnosis of frontotemporal dementia (FTD) were compared with the findings in a control group of 76 individuals without dementia or stroke. A pattern of frontal and temporal atrophy with predominantly frontal white matter changes was found in the FTD patients, and this was significantly different from the radiological findings in the control group. Six of the FTD patients have undergone autopsy. Histopathological evaluation showed a primary cortical degenerative disease (frontal lobe degeneration of non-Alzheimer type) in 3 of them, and primary white matter disorder, mainly frontal, of basically ischemic type (selective incomplete white matter infarction) in 3 of them. MRI could be a helpful tool to support the clinical diagnosis FTD, especially in young patients. MRI may also be helpful for the differentiation of a primary neurodegenerative from a mainly ischemic-vascular type of dementia.
The DNA content of tumor cells in imprints and biopsies from 33 patients with invasive squamous cell carcinoma and 5 patients with adenocarcinoma of the uterine cervix were analyzed by flow cytometric and single cell cytophotometric methods. A correlation of r = 0.83 was found between the modal DNA values obtained by the two methods. Both methods discriminated between diploid and aneuploid tumors and gave comparable but not identical results. Single cell cytophotometric measurements were in this study more reliable than flow cytometric measurements in diploid tumors. Multiple stemlines, small aneuploid peaks and the so called S-phase rate could only be determined by the flow cytometric technique. Flow cytometry seems to be the method of choice in determination of the DNA content in cervical carcinoma cells and cytophotometric single cell measurements are of complementary value.
Flow cytometric measurements of the DNA content were performed in a prospective study of 167 women with invasive squamous cell carcinoma of the uterine cervix. Ploidy level and the proportion of S-phase cells were correlated to age, menopausal age, staging according to FIGO and histopathology. With increasing age a successive shift from a dominance of peri-diploid and peri-tetraploid values to marked aneuploidy was found. Peri-diploid and peri-tetraploid tumors were more often found in premenopausal than in post-menopausal women (p less than 0.001). The mean S-phase rate was significantly higher in women aged 60-89 years than in women aged 20-59 years (p less than 0.01). More aneuploid tumors were found in stages III and IV than in stages IB and II (p less than 0.01). The mean S-phase rate was higher in stages III and IV (20.8%) than in stages IB and II (17.2%) (p less than 0.01). No statistically significant correlation was shown between ploidy level and histopathology or between S-phase rate and histopathology. In 37 patients polyclonal tumors were found. The reproducibility of the method was good (r = 0.99).
Phosphate and TRIS buffers of low molarity commonly used for steroid receptor assays were studied for their pH stability. Increased temperature from 0-37 degrees C lowers the pH of TRIS buffer, while phosphate buffer remains approximately constant. Addition of dextran and charcoal may change the pH unacceptably, depending on the charcoal quality.
Immunohistochemical staining of invasive squamous cell carcinoma of the uterine cervix, stage IB to IV, with monoclonal antibodies against blood group antigens shows retained expression of the antigen in two thirds of the patients, regardless of stage. The over-all 5 year survival of the 115 patients was 60%, 71% in the antigen positive group, 37% in the antigen negative group. The loss of blood group antigen expression thus implies a worse prognosis in all stages of cervical carcinoma and could be used as a predictive marker.