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Biomedical subjects

A Kohno

Publications and source records attributed to A Kohno.

At least 91 records · Page 5Linked to original sources
PubMed1985

Morphologic demonstration of cytoplasmic ASSAM-related antigenic substance (CASSAM) by an immunoperoxidase technique.

Murine senile amyloid protein identified in the senescence-accelerated mouse (SAM) was called ASSAM, and the ASSAM-related antigenic substance was detected in the cytoplasm of hepatocytes, columnar epithelia of the small intestine, and epithelia of the proximal convoluted tubules of the kidney, with the use of an immunoperoxidase method. This substance, called CASSAM (cytoplasmic ASSAM-related antigenic substance), did not stain positively with Congo red nor fibril structure, as determined under an electron microscope. As the ASSAM (senile amyloid) deposition increased with advancing age, CASSAM observed in hepatocytes and columnar epithelia decreased both in SAM-P and SAM-R strains. In the liver of the SAM-P strain in particular, the incidence and intensity in deposition of ASSAM increased rapidly from 5 months of age; on the other hand, CASSAM observed in the hepatocytes decreased rapidly at about the same time. Cycloheximide-treated animals showed a significantly low concentration of SASSAM (serum ASSAM-related antigenic substance) and also a low incidence and intensity of CASSAM observed in the cytoplasm of the hepatocytes and epithelia of the small intestine. In colchicine-treated animals, SASSAM concentration was slightly lower, and the severity of CASSAM observed in the cytoplasm was slightly higher, in the liver and kidney, as compared with control values. CASSAM is assumed to be synthesized in the cytoplasm of the cell and to be secreted alone or in the lipoprotein form into the serum. This CASSAM or lipoprotein including CASSAM is perhaps a constituent of SASSAM (CASSAM is assumed to include apoSASSAM) and the hepatocytes and intestinal mucosal epithelia are possible production sites of apoSASSAM.

Aging↗
PubMed1984

CT findings of atrial myxoma.

The computed tomographic (CT) appearance of six atrial myxomas was analyzed. Five of the myxomas were located in the left atrium and one was in the right atrium. The margin of the myxoma was at least slightly lobulated in five cases and the content was inhomogeneous in all. Calcification was demonstrated in three cases. The site of attachment of the myxoma was demonstrated by CT to be the atrial septum in all cases. The CT findings correlated well with the operative findings. CT revealed prolapse of the myxoma into the ventricle in two cases. This finding was similar to echocardiographic and cineangiographic findings. It is concluded that it is possible with CT to diagnose atrial myxoma by the location and nature of the intracardiac mass and to differentiate it from thrombus.

Adult↗
PubMed1983

Genetic regulation of the class conversion of dsDNA-specific antibodies in (NZB X NZW)F1 hybrid.

To investigate the possible effects of NZW genes on the class conversion of dsDNA-specific antibodies in NZB X NZW (B/W)F1 hybrids, we measured IgM, IgG1, and IgG2 dsDNA-specific antibodies, using the Crithidia luciliae kinetoplast immunofluorescence test, in NZB, NZW, B/W F1 hybrid, B/W F1 X NZB backcross, and B/W F1 X NZW backcross mice at 4, 7, and 10 months of age. The highest serum levels of IgM dsDNA-specific antibodies were observed in NZB mice at the ages tested; however, the amounts of IgG1 and IgG2 antibodies were scanty. In contrast, a large amount of both IgG1 and IgG2 dsDNA-specific antibodies was produced in B/W F1 hybrids, in which the serum IgM antibodies were lower than those observed in NZB mice. NZW mice were virtually negative for these antibodies. Progeny testing suggested that a combined effect of two unlinked dominant genes of the NZB strain determines the production of dsDNA-specific antibodies and that these genes only act to produce IgM antibodies. These traits are to a great degree modified by the NZW loci in B/W F1 hybrids, and a combined effect of two unlinked dominant genes leads to conversion of the class of the antibodies from IgM to IgG, which, in turn, increases the serum levels of dsDNA-specific antibodies. The F1 hybrid of C57BL/6 and NZW strains produced no dsDNA-specific antibodies, indicating that the relevant NZB predisposing genes are required for the NZW gene action. Linkage studies showed that one of such NZW genes is to some extent linked to the H-2 complex on chromosome 17, but not to Mup-1 (chromosome 4) or a coat color locus (chromosome 2). The appearance of IgG dsDNA-specific antibodies correlated well with the incidence of renal disease in B/W F1 X NZB backcross mice.

Animals↗
PubMed1983

Mesenteric Castleman tumor.

Computed tomography in a case of mesenteric Castleman tumor showed a well-circumscribed homogeneous soft tissue density mass with moderate contrast enhancement after injection of contrast material. These findings correlate well with previously reported mediastinal Castleman tumors.

Adult↗
PubMed1982

[Effectiveness of CT in the diagnosis and therapy of malignant thoracic cancer].

Since 1975 computed tomography (CT) has become available for the study of the body. In the thorax CT proves to be valuable to evaluate the lesions of the mediastinum, pleura and chest wall. However, the great difference in attenuation between air-filled lung and other soft tissue structures contributes greatly to the success of conventional diagnostic modalities, i.e. plain chest radiography and conventional tomography in the diagnosis and evaluation of the mass lesions in the lung parenchyma. And the role of CT is less valuable than in the other areas. However, CT is useful to diagnose and evaluate the chest wall or mediastinal invasion as well as mediastinal lymph node involvement. In radiotherapeutic managements of the malignancies in the thorax CT is valuable for the definition of the target volume and physical dose planning because CT provides information on patient outline and other internal structures of interest in addition to the size and shape of the tumor. CT is useful to estimate the efficacy of the conservative treatment, such as chemotherapy or radiotherapy for the thoracic malignancies, especially those in the mediastinum and chest wall. During follow-up study of the pulmonary malignancy conventional radiographic techniques may not be as valuable as before treatment because there is great change of the pulmonary architecture due to fibrosis and/or atelectasis secondary to the treatment. And CT may give valuable information in such cases.

Humans↗