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Biomedical subjects

A Kiss

Publications and source records attributed to A Kiss.

At least 379 records · Page 21Linked to original sources
PubMed1980

Chromosome abnormalities, clinical and morphological manifestations in metamorphosis of chronic myeloid leukemia.

Seventeen cases of chronic myeloid leukemia (CML) in metamorphosis have been investigated by cytogenetic methods. Six patients were studied in slow transformation and others in the terminal blastic phase of the disease. Of these, 3 cases were myeloid, 3 myelomonocytic, 1 megakaryoblastic, 1 promyelocytic and 4 lymphoid in their morphological and cytochemical appearance. Some correlation could be observed between the clinical, morphological, cytochemical and cytogenetic findings of the different subgroups. The results demonstrate the importance of chomosome investigations in the early diagnosis of metamorphosis and in distinguishing the different subgroups of the terminal stage of CML.

Adult↗
PubMed1980

Cloning the modification methylase gene of Bacillus sphaericus R in Escherichia coli.

The gene coding for the sequence-specific modification methylase methM . BspI of Bacillus sphaericus R has been cloned in Escherichia coli by means of plasmid pBR322. The selection was based on the expression of the cloned gene which rendered the recombinant plasmid resistant to BspI restriction endonuclease cleavage. The gene is carried by a 9 kb BamHI fragment and by a smaller 2.5 kb EcoRI fragment derived from the BamHI fragment. The Bsp-specific methylase level was found to be higher in the recombinant clones than in the parental strain. The methylase gene is probably located on the Bacillus sphaericus chromosome, and not on a plasmid known to be carried by this strain. The recombinant clones do not exhibit an BspI restriction endonuclease activity.

Bacillus↗
PubMed1980

Investigation of the active center and catalytic mechanism of porcine kidney aminoacylase: a model of the active center.

Procine kidney aminoacylase (E.C. 3.5.1.14) is inhibited neither by phenylmethylsulfonylfluoride nor by alkylating agents (iodoacetate or iodoacetamide). Therefore reaction mechanisms including the formation of acylenzyme through seryl or cysteinyl side chains are ruled out. The enzyme is a metalloprotein that can be inactivated by ECTA and in which Co2+ is an equivalent substitute for the Zn2+ ion. The two SH groups/subunit of aminoacylase exhibit different reactivites to p-hydroxymercuribenzoate. Modification of the less reactive SH group reversibly inactivates the enzyme. We suggest that this cysteinyl side chain is situated in the active center or in its immediate vicinity. On the basis of our results we suppose a close similarity between aminoacylase and carboxypeptidase A with respect to their active center and catalytic mechanism.

Aminohydrolases↗
PubMed1980

[Treatment of obesity in the elderly with behaviour modification techniques].

Several common methods of treating obesity (diet alone, diet plus medical information, and diet plus behaviour therapy) were compared in a health-spa setting. 107 men (mean age 57.3 +/- 8.0) and 116 women (mean age 61.0 +/- 7.7) participated in the study. Changes in weight during the 26 day treatment period and 3-month follow-up period were registered. The behaviour therapy group showed the greatest weight loss during the treatment period and the least weight gain at follow-up, the difference to the group with medical information was statistically significant. In the behaviour therapy group there were also fewer patients whose weight at follow-up was above their beginning weight.

Aged↗
PubMed1980

Compensatory hyperkinesis of the ventricular walls in acute myocardial ischaemia.

After ligation of the anterior descending coronary artery in dogs, conductance of the left circumflex branch and contractility of the ventricular muscular areas supplied by the left descending coronary artery and its circumflex branch were studied over 60 min. After ligation a considerable increase in contractility of the myocardial areas of the posterior wall with intact blood supply was found and a further increase parallel with a considerably enhanced flow in the circumflex branch. At the same time the contractile power of the anterior ventricular myocardium with a poor blood supply greatly decreased. A close correlation was found between the increase in flow in the circumflex branch and the increase in contractility of the intact areas. The tissue catecholamine level showed a marked increase in the myocardial areas with impaired blood supply.

Acute Disease↗
PubMed1979

CPC-PTA section staining of acid glycans.

A cetylpyridinium chloride-phosphotungstic acid procedure suggested by Kelényi and Kiss (1976) for the staining of mast cell granules in ultrathin sections was slightly modified with regard to pretreatment, pH of incubation, and rinsing of sections. The method also proved suitable for the demonstration of various mucosubstances and of cartilage proteoglycan aggrebates.

Animals↗
PubMed1979

Physical map of the seven ribosomal RNA genes of Escherichia coli.

Escherichia coli DNA was digested with restriction endonucleases BamHI, PstI, EcoRI, SalI, HindIII, XhoI, BglII, SmaI, HpaI and with selected double and triple combinations of the same enzymes. The digests were electrophoresed and hybridized with 32P-labelled ribosomal RNA by using the Southern blotting technique. The resulting bands could be arranged into seven groups, and it was possible to construct a unique physical map of the seven rRNA genes (operons) of the bacterial chromosome. Mapping information obtained on several transducing phages and recombinant plasmids carrying rRNA genes, and mapping data published in the literature helped to determine the final map. The results suggest that phage lambda daroE152 carries a "hybrid" rRNA gene which was probably formed by recombination between two different chromosomal rRNA genes.

Chromosome Mapping↗
PubMed1979

[A ruthenium red-osmium tetroxide section staining method (author's transl)].

We suggest a method for the section staining of polyanions by means of ruthenium red and osmium tetroxide. A 5 min incubation in diluted ruthenium red-OsO4 solution pH = 7.4 of ultrathin Durcupan-sections previously treated with sodium methoxide results in the opaque stining of mast cell granules, cartilage proteoglycans, and various epithelial mucosubstances.

Animals↗
PubMed1979

[On the presence of 20-methylcholanthrene in the atmospheric air (author's transl)].

The strongly carcinogenic polycyclic aromatic hydrocarbon 20-Methylcholanthrene was detected in the urban atmospheric air in Budapest (the capital of Hungary). The main source of this pollution seems to be the motor vehicle traffic, since samples from a heavy traffic junction contained up to six times higher 20-Methylcholanthrene concentrations than samples from a low traffic area. Ultraviolet absorption spectrophotometry yielded similar results. The presence of 20-Methylcholanthrene is apparently connected with the presence of 3,4-Benzpyrene and 1,2-Benzpyrene, respectively.

Air Pollution↗
PubMed1978

Biochemical characterization of the restriction-modification system of Bacillus sphaericus.

A type II restriction endonuclease (endo R . Bsp) has been purified from Bacillus sphaericus to electrophoretic homogeneity. The enzyme appears to be a single polypeptide chain with a molecular weight of 35000. Its pH optimum is around 8.2, it requires 20 mM Mg2+ for optimal activity and it is inhibited by Zn2+. The yield of the enzyme is higher than that of any type II restriction endonuclease so far reported. The enzyme also cleaves single-stranded DNA, albeit at a slower rate. It seems likely that single-stranded DNA is cleaved at the same sequences as double-stranded DNA. Bacillus sphaericus also contains a modification methylase (meth M . Bsp) which completely protects the cell's own DNA against cleavage by its restriction endonuclease. The methylase activity has been partially purified, it copurifies with the nuclease until the next to the last step. The enzyme does not require ATP or Mg2+, it transfers the methyl group of S-adenosyl-methionine to cytosine residues of DNA. As the action of this methylase completely protects any DNA from endo R . Bsp cleavage, it seems likely that the methylase recognizes and methylates the same sequence (dG-dG-dC-dC) as the nuclease.

Bacillus↗