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Biomedical subjects

A Kiss

Publications and source records attributed to A Kiss.

At least 235 records · Page 13Linked to original sources
PubMed1989

[Clinical significance of chromosome number deviations in myelodysplastic syndromes and in acute non-lymphoid leukemia].

The cytogenetic data of 77 patients (47 adults and 30 children) with myelodysplastic syndromes and acute non-lymphoid leukemia are evaluated with regard to the morphological types of leukemia and prognosis. The groups of the adult patients were found to be different in the frequency and types of non-random chromosome aberrations. In patients with secondary leukemias and mutagen-related leukemias the incidence of chromosomal abnormalities was higher than in those with the idiopathic form of the disease. Specific abnormalities were total or partial loss of chromosome 5 and/or 7, and an additional chromosome 8. In contradistinction to these the patients with primary leukemias had specific translocations associated with pseudodiploidy. We found the frequency of aberrations in adults exposed to mutagen agents similar to that in children with ANLL, but the types of aberrations were similar to those of adults without any exposition. Comparing the median duration of remission and survival of patients' groups with different cytogenetic findings we found the chromosome aberrations to be of prognostic value. The present data demonstrate the usefullness of cytogenetic investigations in the diagnosis of the disease and in morphological and etiological classification of patients.

Adult↗
PubMed1989

[Wiskott-Aldrich syndrome in a hetrozygous woman].

The authors report a case of a 20-year old woman, with all the characteristics of the Wiskott-Aldrich-syndrome. The main signs of the disease were recurrent infections, eczema, thrombocytopenia and mild mucosal bleeding. The size of platelets was reduced. Storage pool disease was based on the abnormal platelet aggregation and the lack of dense bodies shown by electronmicroscopic examination. The disturbances of the lymphocytes, neutrophils and monocytes, which resulted in immunodeficiency, could be proven by the immunological tests. This X-linked recessive disorder appeared with typical signs in a heterozygous carrier woman. According to our knowledge, no similar case has been reported before.

Adult↗
PubMed1989

Cloning and structure of the BepI modification methylase.

The gene coding for a CGCG specific DNA methylase has been cloned in E. coli from Brevibacterium epidermidis. The enzyme, named BepI methylase, is probably the cognate methylase of the FnuDII isoschizomer BepI endonuclease isolated from this strain. The expression of BepI methylase in E. coli is dependent on the orientation of the cloned fragment suggesting that the gene is transcribed from a promoter on the plasmid vector. No BepI endonuclease could be detected in the clones producing BepI methylase. The nucleotide sequence of the BepI methylase gene has been determined, it predicts a protein of 403 amino acids (MR: 45,447). Analysis of the amino acid sequence deduced from the nucleotide sequence revealed similarities between the BepI methylase and other cytosine methylases. M. BepI methylates the external cytosine in its recognition sequence.

Amino Acid Sequence↗
PubMed1989

Splenic irradiation as primary therapy for prolymphocytic leukemia.

A patient with prolymphocytic leukemia (PLL), a lymphoproliferative disorder that carries a poor prognosis, is presented. The disease was diagnosed at an early stage and treatment could be delayed for four years. When massive, painful splenomegaly developed, splenic irradiation (SI) was chosen as the primary form of therapy and an excellent systemic response could be achieved. Our observation is in agreement with preliminary studies, which advocate SI as the primary form of therapy in PLL. Furthermore, it is emphasized that an early diagnosis of PLL is necessary to establish its true course and that the prognosis may be better than originally thought.

Humans↗
PubMed1989

Intestinal neuronal degeneration in a patient with chronic idiopathic intestinal pseudoobstruction.

Neuropathological examination of the gut in a patient with chronic idiopathic intestinal pseudoobstruction and temporal lobe epilepsy showed a degeneration of Auerbach's and of Meissner's plexus. The extent of generative changes increased in an aboral to oral direction. Neuronal degeneration was characterized by ballooning of the cytoplasm of the ganglial cells, by a hyperargyrophilia, a shortening and dilation of cell processes, and a progressive fragmentation and loss of axons. In rectal biopsies, a PAS-positive granular material was detected in the cytoplasm of ballooned ganglial cells. A severe peripheral neuropathy with fiber degeneration in the posterior columns and axonal lesions within ventral nerve roots and a more recent fiber degeneration in the lateral columns corresponding to the picture of subacute combined degeneration of the spinal cord and a symmetrical Ammon's horn sclerosis was present. This case of progressive intestinal neuronal degeneration of unknown origin is a newly described condition leading to chronic idiopathic intestinal pseudoobstruction.

Adolescent↗
PubMed1989

Abnormalities of chromosome 1 in relation to human malignant diseases.

Chromosome 1 is known to often be involved in various malignant diseases. Its numerical and structural aberrations have been observed in chronic and acute leukemias and solid tumors as well. Recently five protooncogenes have been assigned to the long and short arms of chromosome 1. The frequent and nonspecific occurrence of chromosome 1 rearrangements in human tumors suggests that they play an important role in the pathogenesis and progression of these diseases. The frequency, types, and time of the occurrence of chromosome 1 aberrations and their relation to the stage of the disease were studied in 317 patients with various malignant diseases. In ten patients nonrandom aberrations of chromosome 1 were observed. Two patients had CML, two PRV followed by ANLL, and the remaining six patients suffered from ANLL, ALL, Burkitt lymphoma, MF, SMMoL, and IRSA, respectively. In six patients, total or partial trisomy of the long arm or of the whole chromosome 1 was present, and in three cases balanced translocations involving chromosome 1 could be found. In the cells of one patient a duplication of the centromeric heterochromatin was seen. We analyzed the breakpoints involved. Finally, the aberrations of chromosome 1 were almost always be observed at the terminal stage of the diseases.

Adult↗
PubMed1989

Transient hyperoxaluria after ingestion of chocolate as a high risk factor for calcium oxalate calculi.

In 6 male subjects the diurnal variation of urinary oxalic acid excretion was studied after ingestion of chocolate, a food stuff rich in oxalic acid. The ingestion of chocolate caused a striking but transient increase in urinary oxalic acid excretion due to its absorption in the upper gastrointestinal tract. The peak excretion rates occurred 2-4 h after the intake of the chocolate. The peak values were 235% of the fasting excretion rate in the trial with 50 g chocolate and 289% in the trial with 100 g chocolate and reached the amounts found in cases with primary hyperoxaluria. The administration of ranitidine had no influence on oxalic acid absorption. The transient hyperoxaluria observed seems to be an important factor for the formation of calcium oxalate calculi in patients on risk for stone disorders.

Adult↗
PubMed1989

Cytochemical studies in the blastic transformation of chronic granulocytic leukaemia.

The cytochemical features of blast cells were studied in 45 patients with blastic phase of chronic granulocytic leukaemia. Various degrees of Sudan black B positivity was characteristic of myeloblastic transformation (23 patients), while in the medullary blast cells of nine patients with myelomonocytic transformation the alpha-naphthyl-acetate esterase showed intensive activity. In two cases the demonstrability of beta-thromboglobulin and factor VIII-related antigen in blast cells showing otherwise PAS, acid phosphatase and alpha-naphthyl-acetate esterase activity referred to megakaryocytic transformation. In six patients with lymphoid blast crisis proliferation of the Sudan negative blast cells with different granular PAS, acid phosphatase and/or beta-glucuronidase positivity was demonstrated. In five cases the cytochemical findings of leukaemic cells indicated biphenotypic and mixed transformation, respectively.

Blast Crisis↗
PubMed1989

Relationship of E1 and E3 regions of human adenovirus 35 to those of human adenovirus subgroups A, C and D.

Cloned PstI fragments of human adenovirus 35 (AV35) genome were compared with the DNA of representatives of human adenovirus subgroups A (type 12), B (type 7), C (types 1, and 5), D (type 8), and E (type 4), using blot hybridization techniques. The E1b region of AV35 was found to be more distantly related to those of other subgroups than E1a regions sequences and examined by others. DNA hybridization was observed only between E1b of AV35 and the DNA of AV4, thus the recombinant constructed might be applied as B-subgroup-specific diagnostic probe. Common nucleotide sequences were detected within the E3 regions of serotypes 1, 4, 5, 7, 8, and 35. On the basis of inter-subgroup homology, and PstI-fragments it may be concluded, that the structure of E3 sequences of AV7 and AV35 DNA are closely related to those of AV3 DNA sequenced by Signäs et al. [18]. E4 regions were compared only of serotypes representing subgroups B, C, and D. These sequences were subgroup specific, similarly to E1b regions.

Adenovirus Early Proteins↗
PubMed1989

[Vacuolated neurons in the superior cervical ganglion in rats after repeated stress].

In the article the authors deal with unsolved problem of the occurrence and character of vacuolated neurons (VN) in autonomic ganglia. They describe their own investigations of vacuolated neurons in the superior cervical ganglion of male rat (Wistar) after repeated stress and fixation of the tissue in glutaraldehyde/OsO4. Vacuolated neurons had a well-preserved structure of ganglion cell in spite of that in cytoplasma they contain vacuoles reaching enormous size. Vacuoles had very characteristic content. They were filled in by content of thin density. Along the membrane of the vacuole were cytoplasmatic extensions, their transverse profiles and isolated invaginations of the membrane of vacuole. From the structure of the vacuole cannot be appreciated the character of the vacuole--whether it is phagosomes or the reservoir of cell metabolite established for its function. Because the vacuolated neurons have the same structure as vacuolated neurons in autonomic pelvic ganglia of the rat, the authors in discussion dealt especially with the results of the investigation of the mentioned ganglia.

Animals↗
PubMed1989

Histochemical observations on bone marrow biopsies embedded in glycol methacrylate (Technovit 7100).

Preparation of sections from undecalcified bone marrow biopsies embedded in glycol methacrylate (GMA, Technovit 7100) is reported. The beneficial effect of vacuum for a better penetration of resin into the hard tissue is assumed. Preserved details of 2- to 3-microns-thick sections of bone marrow specimens from various haematological malignancies yield a proper diagnosis and use of some histochemical methods on immature leukaemic cells is also possible. The use of embedding medium Technovit 7100 in the bone marrow histopathology is widely recommended for its simple, quick usage and non-toxic properties. An additional advantage is that blocks can be cut with ordinary steel knives (Histoknife H).

Acrylates↗