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Biomedical subjects

A Kido

Publications and source records attributed to A Kido.

At least 109 records · Page 6Linked to original sources
PubMed1985

Polymorphism of placental phosphoglucomutase in Japanese.

Polymorphism of PGM1 and PGM3 types was investigated in placental extracts from 127 unrelated Japanese parturients living in Yamanashi Prefecture. The gene frequencies were 0.787 for PGM1(1), 0.213 for PGM1(2); 0.760 for PGM13, 0.240 for PGM3(2), respectively. There was no significant association between the PGM1 and PGM3 systems. The PGM3(2) frequency in the present sample population was higher than those in Kanto District, but lower than those in Kinki District.

Electrophoresis, Starch Gel↗
PubMed1984

Immunologic characterization of human seminal leucine aminopeptidase (LAP) and its medicolegal use.

A new method for identification of seminal stains is described, based on the immunologic demonstration of leucine aminopeptidase (LAP), which is extremely abundant in human semen and specific for the prostate as well as semen. An antiserum against human seminal plasma was obtained by repeated immunization of rabbits with seminal plasma and Freund's adjuvant. Ouchterlony's double immunodiffusion test and Culliford's precipitin electrophoresis were performed to demonstrate specific proteins of seminal plasma. LAP activity was visualized with L-leucyl-beta-naphthylamide as substrate and with Fast Garnet GBC as coupler. The immunologic analysis of LAP produced two precipitin lines with enzyme activity. One was observed in kidney, jejunum, pancreas, prostate, as well as in semen, and was completely absorbed with kidney homogenates. The other was found only in semen and the prostate and was not absorbed with kidney homogenates. When the anti-seminal plasma serum absorbed with the kidney was used, the semen-specific LAP could be demonstrated by precipitin electrophoresis only in seminal stains stored for up to 2 months, whereas it was not demonstrated in stains from other human body fluids. By means of precipitin electrophoresis the detection of the semen-specific LAP was possible at semen dilutions of up to 1:32. The method described here greatly enhances the value of semen identification and is quite recommendable for the examination of stains in medico-legal practice.

Body Fluids↗
PubMed1984

A stability study on Gc subtyping in bloodstains: comparison by two different techniques.

The limits of determination of Gc subtypes in bloodstains were compared between the immunofixation method and the sulfosalicylic acid precipitation method using isoelectric focusing on polyacrylamide gel. By the immunofixation method Gc subtyping in bloodstains was successfully made at 37 degrees C after 7 weeks, at room temperature after 17 weeks and at 4 degrees C even after 25 weeks storage. By the sulfosalicylic method Gc subtypes were no longer able to be determined a few weeks after stain formation. The superiority of the results obtained by the immunofixation method makes it the recommended method for the Gc subtyping from bloodstains in medicolegal practice.

Benzenesulfonates↗
PubMed1984

Effect of antispasmodic drugs on colonic motility. Part I: Laboratory study of the dog.

The inhibitory effect of five antispasmodic preparations, prifinium bromide, hyoscine butylbromide, propinox, trimebutine maleate and phloroglucinol + trimethyl-phloroglucinol on acetylcholine-induced contraction of the colon was investigated in anesthetized dogs. Prifinium bromide was most potent followed by hyoscine butylbromide. The other three preparations had no potent inhibitory effect even when given in a dose of 1000 micrograms/kg. The ED50 was 3.1 micrograms/kg for prifinium bromide and 12.3 micrograms/kg for hyoscine butylbromide; the prolonged time of action at ED50 was approximately 30 minutes for prifinium bromide and about 10 minutes for hyoscine butylbromide. From the dog experiments, it became clear that prifinium bromide and hyoscine butylbromide had a relatively potent antispasmodic effect on the colon.

Acetylcholine↗
PubMed1984

Effect of antispasmodic drugs on the colonic motility. Part II: Clinical study in man.

Four antispasmodic preparations (prifinium bromide, propinox, trimebutine maleate and phloroglucinol + trimethylphloroglucinol) identical to those in Part I, study of the dog, were used. Eight subjects were tested for each preparation and eight subjects for the control. The inhibitory effect of the test preparations on neostigmine-induced hypermotility of the ascending and sigmoid colons were investigated. Prifinium bromide provided a marked antispasmodic effect on the ascending and sigmoid colons; the inhibitory effect of trimebutine maleate was limited to the ascending colon. Propinox and phloroglucinol + trimethylphloroglucinol possessed no clear action. It is estimated that prifinium bromide may be useful in the control of the manifestic state of hypermotility of the colon.

Adult↗
PubMed1984

[A case study with bladder metastasis of renal cell carcinoma and stomach cancer].

A 64-year-old woman received nephrectomy and lymph expurgation surgery for renal cell carcinoma on Jury 1, 1981. The pathologic diagnosis was adenocarcinoma of the clear cell type at Robson's stage 2. She next visited the Department of Gastroenterology complaining of stomach discomfort on November 5, 1981. Stomach cancer of Borrmann's type IV was identified in the lesser gastric curvature, but only biopsy was performed because it was inoperable. The pathologic diagnosis was undifferentiated adenocarcinoma. On January 23, 1982, there was microscopic hematuria. A cystoscopic examination revealed one soy bean-sized, smooth, pedicle tumor to which coagula were partially adhered in the center of the triangular region. After TUR-Bt performed on March 3 the pathologic diagnosis was adenocarcinoma of the clear cell type with no submucosal infiltration. Based on these findings, the patient was diagnosed as having suffered metastasis of renal cell carcinoma to the bladder. She died of bleeding from stomach cancer on June 15. Based on the fact that the tumor was localized in the bladder mucosa, implantation through the urinary tract was strongly suspected as the metastatic route of the renal cell carcinoma to the bladder.

Adenocarcinoma↗
PubMed1983

A new reagent for the rapid determination of total hemoglobin as hemiglobincyanide in blood containing carboxyhemoglobin.

Kinetics of conversion of HbCO to HiCN with various K3Fe(CN)6-KCN reagents was examined photometrically. Complete conversion of HbCO to HiCN required about 8 hr, 150 min, and 50 min with Drabkin's reagent, that of van Kampen and Zijlstra, and that of Taylor and Miller, respectively. The absorbance at 540 nm was stabilized practically in 5 min even in CO-saturated blood when a reagent containing a 1600-fold excess of K3Fe(CN)6 to Hb iron was used. A slight increase in the absorbance was observed around 504 nm in the HiCN solution produced with the reagent. The absorbance at 540 nm in the solution, however, was stable until at least 120 min after mixing. From these findings, a reagent containing a 1600-fold excess of K3Fe(CN)6 to Hb iron and an incubation time of 5 min were used for the complete conversion of HbCO to HiCN. The stabilization of the absorbance at 540 nm was often prolonged to 10 min when the reagent was stored over 2 weeks. The reagent was compared with two other reagents, using 20 blood samples containing various amounts of HbCO, and proved to be suitable for the determination of total Hb in blood containing elevated HbCO as well as in ordinary blood.

Carboxyhemoglobin↗