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Biomedical subjects

A Kido

Publications and source records attributed to A Kido.

At least 91 records · Page 5Linked to original sources
PubMed1987

alpha-L-fucosidase phenotyping in human tissues, dental pulps and hair roots.

Phenotypes of alpha-L-fucosidase (Fu) were demonstrated from tissue samples of spleen, liver, lung and kidney stored for a few weeks at room temperature. Activity was fairly low in pancreas, heart, muscle, skin and brain, and typing was not reliable after 1 week storage. Fu types were detectable from dental pulp tissue of up to 1 week storage. Activity was present in hair root cells, but was extremely low. The results show that the Fu typing may be applicable to individual identification of organ tissues and teeth.

Dental Pulp↗
PubMed1987

C6 phenotyping in sera and bloodstains by isoelectric focusing followed by electroimmunoblotting.

The genetic polymorphism of C6 was investigated in 329 unrelated Japanese individuals using isoelectric focusing in polyacrylamide gels followed by an electroimmunoblotting technique. Besides six common phenotypes C6 A, AB, B, AB2, BB2 and B2, six rare variants were observed. The allele frequencies were: C6*A = 0.4422, C6*B = 0.4757, C6*B2 = 0.0714, C6*A3 = 0.0015, C6*M1 = 0.0046 and C6*B3 = 0.0046. The population data confirmed that the C6*B2 allele is the third common allele characterizing Japanese. The present electroimmunoblotting technique was applied to demonstrate C6 types in dried bloodstains. The C6 types were determined from bloodstains stored at 4 degrees C for up to 10 weeks, at room temperature for up to 2 weeks and at 37 degrees C for up to 4 days. The results show that this component system offers a new powerful means for the medico-legal grouping of bloodstains.

Alleles↗
PubMed1987

Polymorphism of ACP1 by isoelectric focusing: population study in Japanese and phenotyping in bloodstains.

The polymorphism of ACP1 was investigated in 749 unrelated Japanese individuals by isoelectric focusing. The allele frequencies were ACP1* A = 0.2083 and ACP1* B = 0.7917, which were almost similar to those reported in other Japanese subpopulations. The isoelectric focusing technique was successfully applied to ACP1 typing in bloodstains; each phenotype was demonstrated at 37 degrees C for up to 4 weeks, at room temperature for up to 10 weeks and at 4 degrees C for over 16 weeks after stain formation. The isoelectric focusing method proved more useful than conventional electrophoretic methods in medicolegal practice.

Acid Phosphatase↗
PubMed1986

alpha-L-fucosidase phenotyping in human placentae, semen and seminal stains.

The polymorphism of alpha-L-fucosidase (Fu) was investigated in a Japanese population from samples of placentae and semen, using isoelectric focusing. The gene frequencies of placental types were Fu1 = 0.748 and Fu2 = 0.252, and those of seminal types were Fu1 = 0.739 and Fu2 = 0.261. The coincidence in the distribution between the placental and seminal types suggests that the Fu types occurring in placentae and in semen are controlled by the same Fu alleles. The Fu typing was possible in seminal stains stored at 4 degrees C for up to 9 weeks, at room temperature for up to 7 weeks and at 37 degrees C for up to 4 weeks. The Fu types were still detectable at semen dilutions of up to 1:4. This polymorphism would provide a useful genetic marker for the medicolegal grouping of seminal stains.

Female↗
PubMed1986

An endoscopic method to study the relationship between bowel habit and motility of the ascending and sigmoid colon.

Endoscopic retrograde bowel insertion (ERBI), a new method, offers rapid access to the entire colon for pressure sensors. The authors measured the pressure of both the ascending colon and the sigmoid colon and related them to the bowel habits of the subjects. The following groups were studied: control subjects, patients with diarrhea-dominant irritable bowel syndrome, patients with constipation-dominant irritable bowel syndrome, and patients with right-sided diverticular disease of the colon. In patients with irritable bowel syndrome with diarrhea or constipation, colonic motility indices (CMIs) showed a so-called "paradoxical motility" pattern. In patients with right-sided diverticular disease, the CMI was higher in the ascending colon than in the sigmoid colon. The pattern of CMIs for diverticular disease and irritable bowel syndrome varied inversely in the ascending colon and in the sigmoid colon with diarrhea and constipation. These results suggest that the mechanisms of altered bowel habits in patients with these diseases are quite different.

Adolescent↗
PubMed1986

[Estimation of 99mTc-dimercaptosuccinic acid renal uptake using single photon emission computed tomography].

Renal function was assessed by measurement of 99mTc-dimercaptosuccinic acid (DMSA) uptake by the kidney based on the transsectional tomographic image obtained by single photon emission computed tomography (SPECT). The renal uptake was expressed as percentage of the total radioactivity detected in the kidney, the volume of which was measured by convolution method, against the amount dosed. Absorption was corrected by GE-STAR method using cut off level of 42%. In order to determine normal range, measurement was made for 40 kidneys of each of 10 male and female volunteers confirmed of having normal kidneys both morphologically and functionally. The average volume of the kidney was 220.4 ml for the right and 239.3 ml for the left for males, and 205.9 ml and 236.5 ml, respectively for females. The renal uptake of radioactivity (at 2 h after injection), was 26.8% for the right and 27.6% for the left for males, with corresponding figures for females being 26.4% and 27.9%, respectively. Distribution range of renal volume and renal uptake was obtained by bivariate analysis with 90% and 95% probability. From these results, our method of renal function determination based on renal uptake of 99mTc-DMSA obtained from renal transactional tomogram by SPECT is considered to be accurate and potentially useful for clinical purpose.

Adult↗
PubMed1985

Polymorphism of EsD by isoelectric focusing: description of the new allele EsD*Kofu and phenotyping in bloodstains.

The polymorphism of EsD was investigated in 1115 unrelated Japanese individuals by isoelectric focusing. Besides the three common phenotypes two heterozygotes EsD 7-1 and EsD 7-2 were observed. The gene frequencies were: EsD*1 = 0.6234, EsD*2 = 0.3663, and EsD*7 = 0.0103. In addition, a rare variant was detected in a probandus living in the city of Kofu. The family analysis suggested the hereditary occurrence of a new allele EsD*Kofu. The isoelectric focusing method was successfully applied to phenotyping EsD in bloodstains; each phenotype was demonstrated at 37 degrees C for up to 2 weeks, at room temperature for up to 9 weeks, and at 4 degrees C for over 20 weeks after stain formation.

Alleles↗
PubMed1985

Placental-soluble aconitase polymorphism in Japanese.

Polymorphism of soluble aconitase was investigated in 152 Japanese placentae. The allelic frequencies were ACONS1 = 0.951 and ACONS2 = 0.049. ACONS2 appears to be rather high among Orientals including Japanese, while ACONS4 seems to be characteristic for Negroids.

Aconitate Hydratase↗
PubMed1985

A further study on the polymorphism of placental alkaline phosphatase in Japanese.

Polymorphism of placental alkaline phosphatase (PL) was investigated in 120 Japanese placentae by means of starch gel electrophoresis and an improved staining method for the isoenzymes using naphthol AS-MX phosphate as substrate instead of beta-naphthyl phosphate. The present method gave more satisfactory results for the detection of the PL bands. Besides five of the six common phenotypes three different uncommon variants were identified. The gene frequencies of PL types were estimated at PL1 = 0.729, PL2 = 0.054, PL3 = 0.200 and PLv = 0.017, which were very similar to those reported in other districts of Japan.

Alkaline Phosphatase↗