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Biomedical subjects

A Ishida

Publications and source records attributed to A Ishida.

At least 307 records · Page 17Linked to original sources
PubMed1984

[Rhabdomyosarcoma of the bladder in a child: report of a case].

A case of rhabdomyosarcoma of the urinary bladder in an 11-month-old boy is presented. The chief complaint was complete urinary retention and histological examination showed embryonal rhabdomyosarcoma of the bladder. Initially, he was treated with vincristine, actinomycin-D and radiation therapy. This therapy was not effective, and he was next treated with vincristine, bleomycin, and cis-DDP. This therapy was significantly effective, and the tumor became non-palpable on physical examination. After six courses he was discharged and maintenance chemotherapy was continued until the tumor relapsed 1 year later. Finally he died of dyspnea due to diffuse pulmonary metastases approximately 2 years after the first diagnosis. We herein discuss several points which affect the prognosis and the usefulness of chemotherapy, especially combination therapy with vincristine, bleomycin, and cis-DDP for recurrent cases.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1983

Variation of amount of muscle discharges during ballistic isometric voluntary contraction in man.

The effect of force velocity on the relation between voluntary force exertion and amount of muscle discharge was investigated. Surface e.m.g. from the adductor pollicis muscle and voluntary force curve were recorded simultaneously. Examined forces were selected from 500 to 3500 g at the peak force (about 30% of MVC) with time to peak of force curve between 60 ms and 500 ms. The amount of discharge during slow ramp contraction increased with the force increment. In ballistic contractions there was a wide variation in both the amount and rate of discharge. It is suggested that the modes of motor unit activities are different between force exertion with time to peak of less than 150 ms and force exertion with time to peak of over 150 ms.

Adult↗
PubMed1983

Antiallergic agents. 2. N-(1H-tetrazol-5-yl)-6-phenyl-2-pyridinecarboxamides.

A new series of N-(1H-tetrazol-5-yl)-6-phenyl-2-pyridinecarboxamides was prepared to determine the effects of substituents on the benzene and pyridine rings on antiallergic activity in the rat passive cutaneous anaphylaxis (PCA) assay after oral administration. One member of this series, N-(1H-tetrazol-5-yl)-4-methyl-6-[4-(methylamino)-phenyl]-2- pyridinecarboxamide (231), has an ED50 value of 0.8 mg/kg po and is 85 times more potent than disodium cromoglycate (DSCG) on intravenous administration. Further evaluation of 231 as a clinically useful antiallergic agent is in progress.

Animals↗
PubMed1983

Modulation of the cytotoxicity of the antitumor antibiotic peplomycin by membrane-interacting drugs and by increased levels of calcium ions.

The cytotoxic effect of peplomycin (PEP), a new derivative of bleomycin glycopeptide antibiotics, toward HeLa cells and mouse FM3A cells was markedly enhanced by combination of PEP with non-toxic doses of the membrane-interacting drugs verapamil (0.1-0.2mM) and dibucaine (0.15-0.25mM), or with CaCl2 (10-16 mM). Treatment with verapamil or CaCl2 following PEP treatment also effectively enhanced the cytotoxic effect of PEP, suggesting an interaction with PEP-induced damage. Cellular uptake of PEP did not increase in dibucaine-treated cells, suggesting no correlation between membrane permeability to PEP and enhanced cytotoxicity. Increased Ca2+ did not enhance the cytotoxic effects of adriamycin, mitomycin C, cis-diamminedichloroplatinum (II), vinblastine or macromomycin, thus suggesting a unique interaction with PEP. The enhancing action of verapamil and Ca2+ was greatly promoted by 41 degrees hyperthermia, although 41 degrees alone scarcely enhanced PEP cytotoxicity. Cytochalasin B and alpha-tocopherol, but not cytochalasin D, reversed the enhanced cytotoxicity produced by PEP and verapamil or dibucaine, but did not reverse the enhanced cytotoxicity by PEP combined with increased Ca2+. The enhancing action of Ca2+ was, however, antagonized by ruthenium red and lanthanum chloride, which are potent inhibitors of Ca2+ uptake by cells, or by magnesium chloride. Verapamil and increased Ca2+ promoted the decomposition of the DNA-membrane complex induced by PEP in HeLa cells, and also impaired the regeneration of the decomposed DNA complex.

Animals↗
PubMed1983

Differential enhancement of cytotoxicity by combination of the carcinostatic agent benzaldehyde and hyperthermia in simian virus 40-transformed and normal cell lines.

The effect of the carcinostatic drug benzaldehyde (BA) on hyperthermia-induced cytotoxicity was studied in various types of cultured mammalian cell lines by colony formation assay. The treatment of HeLa cells with nontoxic doses of BA (1 to 4 mM) at 42 and 43 degrees for up to 3 hr induced enhancement of cell killing. BA-induced enhancement of hyperthermic cell killing was much greater in SV40-transformed rat fibroblast cells than in the untransformed cell line. This BA-induced differential enhancement of hyperthermic cell killing increased as the time and dose of BA treatment were increased at elevated temperatures. For example, BA (2 mM) treatment for 4 hr at 42 degrees decreased the survival of transformed cells to 2 X 10(-4), but decreased the survival of untransformed cells to only 0.1. Furthermore, BA (4 mM) treatment for 2 hr at 42 degrees reduced the survival of transformed cells to less than 10(-5), but reduced the survival of untransformed cells to 5 X 10(-2). Differential enhancement of hyperthermic cytotoxicity by BA was also evident between normal human diploid fibroblast W138 cells and its SV40-transformed cell line, although the magnitude of the enhancement was less than that observed with rat cell lines. The determination of BA remaining in the medium after incubation with transformed and untransformed rat cells at 37, 42, and 43 degrees suggested that greater amounts of BA reacted with or were consumed by transformed cells irrespective of temperature.

Animals↗