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Biomedical subjects

A I Esakov

Publications and source records attributed to A I Esakov.

At least 19 recordsLinked to original sources
PubMed1990

Litorin and litorin-albumin conjugate as effective regulators of body temperature in rats.

Intraperitoneal injection of the bombesin-like peptide litorin (0.5 ml of a 10(-5) M solution) led to a short-lived decrease in the body temperature (maximum of 0.9 degrees C) of laboratory rats. In contrast, immunization with litorin conjugated to bovine serum albumin (BSA) led to an increase in body temperature from 36.6 +/- 0.1 to 37.2 +/- 0.1 degrees C that persisted for at least 4 weeks and which was probably due to the binding of endogenous litorin by the newly formed antibody. These results suggest that litorin is involved in the physiological regulation of thermal homeostasis. The temperature of control rats immunized with Freund's adjuvant and BSA without litorin fluctuated around normal at 36.6 +/- 0.1 degrees C over this period. Rat body weight increased evenly during the experiment in both the experimental animals and the two control groups. However, the volume of food intake in the hour following 24 h food deprivation was lower in rats immunized with conjugated litorin than in the control groups.

Animals↗
PubMed1989

[Intake of water and sodium chloride solution by the rats after central administration of litorin and immunization with litorin-bovine serum albumin conjugate].

Central injection of litorin suppressed water and sodium chloride solution intake, and also decreased the preference of saline of unrestrained rats during 1 hour test. Active immunization of animals with litorin-bovine serum albumin conjugate led to the augmentation of their daily saline preference. Immunized rats increased also avidity to salt after deprivation, though the consumption volume of liquids decreased. The data, obtained on the immunized animals prove the importance of definite level of endogenous bombesin-like peptide in the salt appetite of rats. The central effect of litorin is discussed in the aspect of changing by it the evaluation of gustatory information.

Animals↗
PubMed1986

[Pharmaco-histochemical study of monoamine-containing cells in the taste buds as affected by capsaicin-induced substance P deficiency].

A clearly positive correlation between the level of substance P (SP) in the taste nerves and cellular serotonin content in the taste buds has been established using fluorescent-histochemical technique. In chronic SP deficiency caused by capsaicin, cellular serotonin content was considerably decreased due to the disturbances in its synthesis and uptake. The injection of exogenous SP resulted in the recovery of the initial serotonin level. The data obtained confirm an important role of SP in the maintenance of the optimal cellular serotonin level owing to the activation of monoamine synthesis and uptake.

Animals↗
PubMed1986

[Histoarchitectonics of the cholinergic innervation of the frog tongue].

Acetylcholinesterase (AChE) activity was studied in dorsal tongue surface structures and in both tongue nerves (hypoglossal and glossopharyngeal) of frogs (Rana temporaria). AChE was found in nerve fibers of fungiform and filiform papillae, blood vessels, glandular ducts of tongue mucosa, both nerve fibers and also in the bodies of cholinergic neurons in subepithelial connective tissue and along the glossopharyngeal nerve. Their parasympathetic origin was suggested. The experiments with butirilthiocholin have revealed no activity of non-specific cholinesterases in the above structures. Possible role of cholinergic system in the regulation of tongue receptor function is discussed.

Acetylcholinesterase↗
PubMed1985

[Activating effect of cholecystokinin-pancreozymin on the taste-receptor system of the rat].

The effect of the intraperitoneal injection of 10(-5) M cholecystokinin-pancreozimin solution on the taste total impulse activity of the rat chorda tympani was studied. The taste responses to sodium chloride and sucrose increased within 5 min and responses to quinine increased within 30 sec after the peptide injection. Responses to citric acid solution did not augment significantly. The augmentation of the taste responses under peptide injection can be blocked by cutting the chorda tympani nerve or subepithelial administration of atropine solution.

Animals↗
PubMed1984

[Effect of substance P on the monoamine-containing cells of the taste buds].

The effect of substance P (SP) on monoamine-containing cells of the frog taste buds was studied by fluorescent microscopy. Intraperitoneal injection of SP resulted in a gradual increase of cell serotonin content. In monoamine deficiency caused by previous injection of rausedyl, SP favoured the recovery of the serotonin level to initial. When SP and rausedyl were used combined, SP protected the serotonin-containing cells from the depleting effect of rausedyl. The functional role of SP in the taste apparatus is discussed.

Animals↗
PubMed1983

[Effects of colchicine, cyclic nucleotides and biogenic amines on the chemoreceptor apparatus of the tongue].

Colchycine considerably decreased sensitivity of the Rana temporaria tongue's chemoreceptors to adequate stimulation. The effect is reversible and can be abolished by administration of drugs initiating setting of the cells' microtubular apparatus: 3',5'-cAMP, theophylline, adrenaline as well as by activation of the sympathetic system. The restoring effect of these agents is specific since 2',3'-cAMP, 5'-AMP, serotonin, inosine, acetylcholine and 3,'5'-cGMP prove ineffective in restoring the reactions after colchycine administration although they do alter the activity of the receptors in normal conditions. The revealed colchycine-sensitive, 3',5'-cAMP-dependent process seems to be associated with the microtubular apparatus of the receptor cell. Its role in the receptor structures is discussed.

Adenosine Monophosphate↗
PubMed1983

[Effect of antibodies against tubulin on the activity of taste receptors].

Electrophysiological studies were carried out to investigate the effect of monospecific tubulin antibodies on the activity of the receptor gustatory apparatus of Rana temporaria under adequate stimulation of receptors with NaCl and glucose solutions and water. There was a significant decrease in receptor responses to NaCl and water stimulation after administration of antibodies. Glucose responses were less prone to the effect of antibodies. The authors discuss the mechanism of the involvement of the microtubules-tubulin system found in receptor formations into the peripheral mechanisms of chemoreception.

Animals↗
PubMed1983

[Electrical response of frog lingual epithelium to stimulation of the gustatory nerve].

Electrical stimulation of the gustatory nerve evoked a positive electrical response of the outer surface of the tongue (relative to its inner layers). The amplitude and the rate of rise of the response grew and the latency fell with increased frequency of stimulation. The response originated in the superficial epithelial layer (30-60 micrometers) and was produced by a decrease of "resting potential" (negative-outside) of this layer. The response was accompanied by a strong decrease of the superficial tongue layer resistance and arose probably as a result of short-circuiting of the epithelial layer by high-conductive nonspecific channels. Similar response accompanied by a decrease of the superficial tongue layer resistance was recorded with adequate stimulation (by acetic acid vapours). The results are compared with the response of the frog skin to the stimulation of cutaneous nerve.

Animals↗
PubMed1982

[Recovery of colchicine-blocked chemoreceptor reactions of the frog tongue as affected by electrical stimulation of the sympathetic nervous system].

Under adequate stimulation of tongue chemoreceptors with feeds synchronous recording of the total afferent impulse activity of the glossal branches of Rana temporaria glossopharyngeal nerves was used to demonstrate the facilitation effect of electric stimulation of the I sympathetic ganglion on the receptor reactions in health and after treatment with colchicine provoking a considerable weakening of the reactions. The data are discussed in the light of the adaptation-trophic effect of the sympathetic system at the level of molecular-metabolic intracellular processes. No poisoning effect of alkaloid on the sympathetic fibers has been recorded.

Animals↗
PubMed1980

[Possible role of sympathetic influences and cAMP in centrifugal inhibitory control of the gustatory receptor apparatus].

The electrophysiological analysis of the taste total impulse activity of the rat revealed the inhibitory influence of the gastric distention on the salt and sour taste responses to be decreased by cutting of the neck sympathetic nerve and prevented by the propranolol injection into the taste epithelium. Salt and sour taste responses were diminished by the injection of isoproterenol, cAMP, dibutyril--cAMP, and the inhibitors fo phosphodiesterase theophilline and paraverine; the activator of phosphodiesterase nicotine acid increased the taste responses. The finding suggest that the adrenergic transmitters and cAMP take part in the actualization of the inhibitory centrifugal influences.

Animals↗
PubMed1979

[Development of accessory structures of the mechano- and chemoreceptor apparatus of the tongue during rat ontogeny (scanning electron microscopy)].

The dynamics on the formation of different types of mechno- and chemosensitive papillae in the rat tongue has been studied during different periods of postnatal ontogenesis by means of scanning electron microsopy. Asynchronous maturation of these papillae has been demonstrated, they perform certain functions during the early postnatal ontogenesis and reach their functional maturity by the age of 3 weeks when animals go over from suckling to solid food.

Animals↗
PubMed1979

[Morphofunctional characteristics of the accessory structures of the cat lingual receptors].

By means of scanning electron microscopy detailed topographic map of the cat tongue was made. It was demonstrated that besides three types of chemoreceptor formations, the dorsal surface of the cat tongue possess the same amount of additional mechanoreceptor structures. By means of registration of afferent impulses, from the lingual nerve fibres at their adequate stimulation, it was possible to determine functional role of some of them.

Animals↗
PubMed1978

[Possible nature of the biogenic amine in the dumbbell-shaped cells of frog taste buds].

An attempt was made to identify specific monoamines contained in the dumb-bell shape cells of the frog taste bud by means of histochemical analysis. It was shown by fluorescent microscopy that preliminary administration of exogenous serotonin into the blood channel of frog tongue resulted in a sharp increase of specific fluorescence of the dumb-bell shape cells, whereas serotonin synthesis inhibition with p-chlorphenylalanine led to reduction and elimination of specific fluorescence. It was concluded that-specific monoamine of the dumb-bell shape cells was possibly of serotonin-like nature.

Animals↗