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Biomedical subjects

A Fuchs

Publications and source records attributed to A Fuchs.

At least 127 records · Page 7Linked to original sources
PubMed1991

DNA flow cytometric evaluation of serous and mucinous cystic neoplasms of the pancreas.

There have been few reports of flow cytometric studies of pancreatic neoplasms, whether endocrine or exocrine. In this report, the DNA content of three mucinous and seven serous cystic tumors of the pancreas was evaluated by flow cytometry on formaldehyde solution-fixed, paraffin-embedded tissue. Two serous cystadenomas were aneuploid, with the remaining five serous and all three mucinous being diploid. All cases had a low S phase (ranging from 0.1% to 3.1%), indicating neoplasms with a low turnover. From these results, it appeared that DNA flow cytometry as an independent factor did not discriminate between the benign pancreatic serous cystadenomas and the more aggressive mucinous cystic neoplasms.

Adult↗
PubMed1990

Synthesis of species-specific stress proteins by virulent strains of Listeria monocytogenes.

Listeriolysin is a virulence factor that appears to be necessary for the intracellular survival of Listeria monocytogenes. As shown in this investigation, listeriolysin is produced in only small amounts by clinical isolates of L. monocytogenes belonging to the serogroup 1/2a, but its synthesis can be induced by heat shock and to a lesser extent by oxidative stress. In addition to about 15 heat shock proteins that appear to be common to L. monocytogenes and Listeria species that are nonpathogenic for humans, at least five heat shock proteins are specifically coinduced with listeriolysin in all L. monocytogenes strains under heat shock conditions but not in the other Listeria species. One type of L. monocytogenes mutant blocked in the expression of listeriolysin failed to synthesize several of these specific heat shock proteins.

Bacterial Toxins↗
PubMed1990

Detection and interpolation of outliers in biosignals.

Detection of outliers in recorded biosignals and their interpolation is described. The detection of outliers is based on the simple rule of 3 sigma. The subsequent interpolation is performed according to the method proposed by Brubacher and Wilson for autoregressive processes. The output of the suggested procedure can be treated by standard programs of statistical analysis as long as the influence of outliers is eliminated. The program in Fortran IV developed in the Institute of Hygiene and Epidemiology, Prague, performs automatically the detection of outliers in an input signal and their replacement by means of the proposed interpolation method.

Animals↗
PubMed1989

Reconstitution of the thermostable trimeric phage P22 tailspike protein from denatured chains in vitro.

Intermediates in the intracellular chain folding and association pathway of the P22 tailspike endorhamnosidase have been identified previously by physiological and genetic methods. Conditions have now been found for the in vitro refolding of this large (Mr = 215,000) oligomeric protein. Purified Salmonella phage P22 tailspikes, while very stable to urea in neutral solution, were dissociated by moderate concentrations of urea at acidic pH. The tailspike protein was denatured to unfolded polypeptide chains in 6 M urea, pH 3, as disclosed by analytical ultracentrifugation, fluorescence, and circular dichroism. Upon dilution into neutral buffer at 10 degrees C, the polypeptides fold spontaneously and associate to form trimeric tailspikes with high yield. Like native phage P22 tailspikes, the reconstitution product is resistant to denaturation by dodecyl sulfate in the cold and displays endorhamnosidase activity. Sedimentation coefficients, electrophoretic mobility, and fluorescence emission maxima of native and reconstituted tailspikes are identical within experimental error. By characterization of intermediates, localization of temperature-sensitive steps, and analysis of the effect of previously identified folding mutations, the reconstitution system described should allow comparison of in vivo and in vitro folding pathways of this large protein oligomer.

Electrophoresis, Polyacrylamide Gel↗
PubMed1989

Spontaneous motor activity in healthy volunteers after single doses of haloperidol.

Video recordings of the spontaneous motor activity of 6 healthy volunteers after treatment with 0.75 mg haloperidol i.v., or placebo, were transcribed into a time-series protocol of motor behaviour. Characteristic changes seen after the injection of haloperidol consisted in a reduction of the motility of the extremities and prolongation of the phases of both movement and immobility of the head. The tested dose of haloperidol induced a distinct rise in serum prolactin and sedation, but had no effects on the pharmaco-EEG or on the critical flicker-fusion frequency. Analysis of the motor phenomena evoked by neuroleptics in healthy persons and in patients may help, on the other hand, to establish correlations with the motor effects observable in preclinical investigations in animals and might also contribute towards the elucidation of the extrapyramidal side-effects of these drugs.

Adult↗
PubMed1988

Pattern recognition and associative memory as dynamical processes in a synergetic system. I. Translational invariance, selective attention, and decomposition of scenes.

We consider a model for associative memory and pattern recognition which was devised by Haken (1987b). This model treats the activity of the neurons as continuous variables and exploits an analogy with pattern formation in synergetic systems. The capability of such a system to act as associative memory is demonstrated by the reconstruction of faces which are partially offered to the system, and which are restored by the corresponding dynamical process. We demonstrate how this model can be cast into a form which is translation invariant and how partially hidden faces in scenes can be recognized by means of the control of attention parameters of specific patterns.

Cybernetics↗
PubMed1988

EEG as a neurotoxicological indicator.

In living organisms both chemical agents and physical factors [16] may produce neurophysiological change that affects EEG activity. EEG signals are very suitable for non-invasive measurement of CNS reactions, but quite complicated equipment is necessary for measurement and analysis. We have implemented a system that permits the study of EEG changes both in time and frequency domains using broad-band analysis or fast Fourier transformation (FFT). Experimental animals were influenced by high doses of toxic agents (CO, CS2, barbiturates, pesticides), drugs and both non-ionizing and ionizing radiation. The EEG changes reflecting the influencing factor, respectively its quantity may be divided into several classes: (1) appearance of new activities; (2) disappearance of some activities; (3) increase of amplitudes, respectively spectral power densities (SPD) in certain frequencies; and (4) decrease of amplitudes, respectively SPD in certain frequencies. All changes are related to the controls, i.e. to the relatively normal state of CNS. Furthermore it is possible to investigate the temporal dynamics of these changes. Physiological concordance of these findings is sometimes possible from clinical analogues, but in other cases is unknown and considerable effort will be necessary to elucidate these correlates. Anyhow in some toxic substances, the EEG may be quite insensitive as an indicator of neurotoxicity. The best way to solve these problems is to collect sufficient experimental data for complex analysis. Although few relevant data are currently available, temporal and frequency domain measures of EEG activity appear to have promise as neurotoxicity indicators.

Animals↗
PubMed1988

The two- and three-dimensional structure of the microcirculation of the chick chorioallantoic membrane.

The chick chorioallantoic membrane (CAM) is a common model for studying biological processes, but descriptions of the CAM circulatory system and especially experimental preparations of the CAM in shell-less eggs are both scant and controversial. We studied the CAM structure and the three-dimensional spatial configuration of the CAM vessels using five methods: in vivo stereoscopic observations, whole-mount preparations, histological sections, corrosion cast microinjection techniques, and the reconstruction of a three-dimensional wax model. Our findings show that the CAM consists of a superficial two-dimensional layer composed of a network of a very dense capillary mesh, floating over and enclosing a deeper three-dimensional space in which medium and larger free-floating vessels are seen to supply and drain the superficial layer. It is interesting to note that no tips or sprouts of blood vessels were observed during the development of the CAM vessels. In a shell-less egg preparation, the capillaries were found in the mesoderm layer of the CAM and not in or superficial to the ectoderm as is the case in the CAM of the intact egg which adheres to the shell membrane.

Allantois↗
PubMed1988

[Characterization and detection of bulimia in the city of Buenos Aires].

A screening of Bulimia, an eating disorder associated to Obesity and Anorexia Nervosa, has been carried out in the city of Buenos Aires. The data were obtained by means of the questionnaire of Pope & Hudson, administered to young women who attend diet and physical activity programs. Control groups were constituted by female university students and boutique employees. The results indicate that diet and physical fitness searchers are groups at risk of Bulimia. It is concluded that proper preventive measures can be taken by informing and training the personnel, professional or not, in charge of those institutions, and also by promoting the adequate information within the population.

Adolescent↗
PubMed1988

[Effect of in vivo nuclear magnetic resonance tomography on somatosensory and visual evoked potentials in the human].

By means of registration of somatosensory (SSEP) and visual (VEP) evoked potentials under the influence of different magnetic and electromagnetic fields of Magnetic Resonance we investigated the influence of Magnetic Resonance Imaging (MRI) on human nervous system (all measurements with an 1.0 T MR imager are performed outside and inside the static magnetic field, before and after MR imaging). In vivo experiments on 20 healthy volunteers show in SSEP registrations as well as in VEP-registrations no measurable influence on nerve conduction. The measured latencies before and after MR imaging are found inside the standard values which are valid for healthy volunteers. In vivo experiments at field-strengthens up to 1.0 Tesla show no significant changes of the central and peripheral conduction velocity with human beings.

Adult↗
PubMed1987

Biological assessment of exposure to antimony and lead in the glass-producing industry.

The oxide of trivalent antimony is used in the glass-producing industry as a refining agent and as a glass colouring. The batch contains up to 2% Sb2O3, a substance which has shown carcinogenic properties in animal experiments. The internal levels of antimony and lead in blood (SbB and PbB) as well as the excretion with the urine (SbU and PbU) were determined by hydrid and electrothermal atomic absorption (HY-AAS and ET-AAS), respectively. In addition, measurements of airborne Sb2O3-concentrations were performed. The 109 volunteers were employed in four different fields: melting area, batch bunker, glass-washing area, and transport/maintenance. Differences between the concentrations of antimony and lead in blood and urine with respect to the fields of activity were evaluated statistically. The highest values of airborne Sb2O3, with up to 840 micrograms/m3 (TWA), were detected in the batch bunker. Correspondingly, significantly enhanced SbU-values from 1.5 to 15.7 micrograms/l (median: 5.0 micrograms/l) were found in specimens collected from the batch mixers. In the same group, the lead excretion (PbU) with values from 9 to 110 micrograms/l (median: 43 micrograms/l) was also found to be the highest. Due to the fast renal excretion of antimony, the determination of SbU is useful for biological monitoring.

Adult↗
PubMed1987

The anti-proliferative effect of lithium chloride on melanoma cells and its reversion by myo-inositol.

The effect of LiCl on melanoma cell growth and differentiation was studied in mouse and human melanoma cell lines. LiCl markedly inhibited B16 and HT-144 melanoma cell growth in vitro. Clonogenicity in soft agar of the melanoma cells was also markedly inhibited by LiCl. Pretreatment of B16 mouse melanoma cells with LiCl delayed the appearance of melanoma tumours in syngeneic mice. Growth inhibition of cells was accompanied by morphological and biochemical alterations. LiCl induced cell enlargement and formation of dendrite-like structures. The activity of NADPH cytochrome c reductase, an enzymatic marker of endoplasmic reticulum was significantly (2-3 fold) increased. Addition of myo-inositol to cell cultures partially reversed the anti-proliferative and morphological effects of LiCl on melanoma cells. This finding may suggest that the anti-proliferative effect of LiCl is related to its effect on phosphatidylinositol metabolism.

Animals↗
PubMed1987

Beta 2-microglobulin amyloidosis (AB2M) in patients undergoing long-term hemodialysis. A new type of amyloid.

Amyloidosis has been increasingly recognized in association with renal failure and chronic hemodialysis. This report describes three patients who had long-term hemodialysis (between 7-18 years), in whom deposits developed of a new type of amyloid of beta 2-microglobulin origin. Beta 2-microglobulin amyloid (AB2M) was found in multiple organs, i.e., bone, subendocardium, gastrointestinal blood vessels, tongue, and carpal tunnel connective tissue. AB2M displayed characteristic amyloid features on conventional light and polarized microscopic examination after congo red staining. However immunostaining with anti-amyloid A protein, kappa, and lambda antisera were negative. The studied material reacted positively with beta 2-microglobulin antisera, identifying AB2M in all three cases. Ultrastructural study revealed an unusual curvi-linear fibrillar configuration. AB2M appears to be a new subtype of systemic amyloidosis secondary to renal failure and long-term hemodialysis.

Adult↗