We analyzed whether normal human hepatocytes, which normally do not display Class II major histocompatibility complex antigens, can be induced to express them in vitro, and whether this induction has an in vivo counterpart in chronic liver diseases. While both alpha- and gamma-interferon induced expression of Class I antigens, only gamma-interferon induced expression of Class II antigens on hepatocytes in vitro. Recombinant interleukin 2 had no effect on major histocompatibility complex antigen expression. Both Class I and Class II antigens could be detected by indirect immunofluorescence on hepatocytes from patients with various forms of chronic liver disease, regardless of etiology. These findings suggest that gamma-interferon produced by T lymphocytes that infiltrate the liver during the course of chronic hepatitis induces Class II major histocompatibility complex antigen expression and may endow the hepatocytes with the capacity to perform accessory (antigen-presenting) cell functions.

Antibodies, Monoclonal↗

PubMedDate not supplied

Blood levels of histamine, IL-1 beta, and TNF-alpha in patients with mild to moderate Alzheimer disease.

In this study, we have evaluated the levels of blood histamine, serum interleukin-1 beta (IL-1 beta), and plasma tumor necrosis factor-alpha (TNF-alpha) in 20 patients with mild to moderate Alzheimer disease (AD; 13 early onset and 7 late-onset AD subjects) and in 20 age-matched control subjects (C). AD patients showed higher concentrations of histamine (AD = 452.9 +/- 237.9 pmol/mL; C = 275.3 +/- 151.5 pmol/mL; p < 0.05) and IL-1 beta (AD = 211.2 +/- 31.1 pg/mL; C = 183.4 +/- 24.4 pg/mL; p < 0.01), and lower values of TNF-alpha (AD = 3.59 +/- 2.02 pg/mL; C = 9.47 +/- 2.64 pg/mL; p < 0.001) than elderly controls. Increased levels of histamine and decreased levels of TNF-alpha were observed in both early onset AD (EOAD) and late-onset AD (LOAD) patients, but only EOAD subjects had elevated serum IL-1 beta values compared with age-matched controls. Age negatively correlated with histamine (r = -0.57; p < 0.05) and positively with IL-1 beta levels (r = 0.48; p < 0.05) in healthy subjects, but not in AD, whereas a positive correlation between TNF-alpha scores and age was only found in AD patients (r = 0.46; p < 0.05). Furthermore, histamine and TNF-alpha values correlated negatively in AD (r = -0.50, p < 0.05). In addition, cognitive impairment increased in patients with lower TNF-alpha and higher histamine and IL-1 beta levels, as indicated by the correlations between mental performance scores and histamine (r = -0.37, ns), IL-1 beta (r = -0.33, ns) and TNF-alpha levels (r = 0.42, p < 0.05). Finally, histamine concentrations decreased as depression scores increased in AD (r = -0.63, p < 0.01). These data suggest a dysfunction in cytokine and histamine regulation in AD, probably indicating changes associated with inflammatory processes.

Age of Onset↗

PubMedDate not supplied

[How can psychological help be provided for the patient/caregiver tandem home?].

INTRODUCTION: Elderly people and their family helpers are often isolated at home and do not have access to the professional psychological help that they require. For an elderly population, the trips to consulting rooms are difficult, tedious and expensive. Besides, maintaining a patient at home is heavy to manage for close relatives because of the organization, financial issues and above all the risk of psychological burnout. The literature shows us that psychological assistance is more common at distance from home, in hospitals, in special institutions or specific organizations. However, there is a clear need of help at home. We propose to develop psychological assistance at home for the patient/helper tandem in cases of dementia. This prospective study reports three different cases. METHOD: This study is a qualitative pilot study. Participants characteristics: patients were diagnosed with severe dementia, assistance showed deep fatigue of the helper, both members of the tandem lived at home, age above 69 years, the eed for nursing care at home. THERAPEUTIC ASSESSMENT: The psychological clinician acts after proposing his helpand not on a clear request from the patient. He will meet his patient 7 times during 4 months. The meetings always take place in the same place and last 45 minutes. Confidentiality during the meeting is explained and guaranteed so the patient can speak openly. EVALUATION: An independent psychologist assessed the monitoring in the hospital. Our methodology included two assessments: the first, assessing the task of the helper before the evaluation and the second taking into account a new measurent of the task and the opinions of the participants following a semi-directed interview. The evaluation of the task was performed using Zarit's scale. The evaluation was also based on the clinical observation of the psychologist. RESULTS: Organization of the monitoring: one meeting per week was sufficient for all participants with a minimal duration of 45 minutes. The fact that the meetingsrook place at home was appreciated, because of their simple and convenient organization. The patient/helper tandems in those suffering from advanced dementia could only have taken place in the home because transport any where else would have beent too difficult for them. The sessions during this research study were free of charge. Two out of three participants were ready to pay so long as the study could continue. The managers were bothered a few times by phone calls and/or unexpected visits, invitations for a cup of tea and requests for a small service (like mailing a letter). Assistance for the caregiver: all the caregivers declared that assistance was a personal improvement in a period of doubt, loss of self-confidence and isolation. Furthermore, clinical observation of the therapeutic assistance appears to show that psychological help at home could lead to the improved psychological function of the helper. This was emphasized when we established the limits of the caregiver/patient relationship. We observed a better balance in the input of investing and de-investing and better acceptance of the identity modifications which were required for the caregiver. Assistance for the patient: we believe that this sort of intervention has positive effects on the patients themselves. This care at home protects the destructurated identity of the patients and their intimity. Moreover, intrapsychic tension can be lowered by being shared with the psychologist. Assessment of the burden: among the three patients who wete studied, the burden was unaltered in one case (43/88; 43/88), significantly improved in one case (41/88; 24/88) and remained light in one case (18/88; 16/88). DISCUSSION: We discovered that Zarit's test showed some limits. The time to complete the test is quite long and tedious for caregivers. Some questions are too direct and can put the caregivers in a guilty position. The mini Zarit version with only seven items, appears more satisfactory because it's shorter and provokes less guilt. The other point concerns the therapeutic frame at home. The usually represents all the constants of the therapeutic process including the role of the psychologist and all the items that refer to space, time, management of the timetable, payement and interruptions in care. Could the fact of being at home be harmful for the therapeutic process? Of course, the place of residence is less neutral than a office in an institution and the superposition of the frame of life and of the therapeutic frame raises lots of questions for the psychologist: entering a private house is like entering a private life, which is not without consequences on the follow-up. We are here far from a classical frame of therapeutic interviews, so the frame must be clearly defined. CONCLUSION: This prospective study leads us to the conclusion that the superposition of the frame of life and of the therapeutic frame represents a limit to psychotherapy but is not exclusive of psychological support at home. As a supplement to this face to face follow up at home, we could imagine other ways of providing such psychological support, by phone or by telemedicine for instance. Could the new technologies of communication help to compensate the lack of means in favour of the caregivers at home? Although these new technologies are more dedicated to institutions than to providing care at home, could they not be helpful for organizing psychological help at home? However, in order to validate such devices, they need to be fried and assessed at home.

Adaptation, Psychological↗

PubMedDate not supplied

[The globalization of health].

In this article diverse aspects of the relationship between health and globalization are explored. Different dimensions of globalization (economic, technological, cultural and political) are considered. Aspects of its effects on health (epidemiological, ethical and environmental), as well as its relationship with public health, power distribution and equity are discussed. Data that demonstrate the globalization of risks and of diseases, due to the current model of international relations and geographical mobility, are analyzed. The article defends the globalization of health and integrates renewed concepts and scientific advances in public health with politics, social strategies and new organizational forms of the practice of public health. Finally, we discuss the opportunities that have been provided by globalization since the middle of the last century for redefining world government and for developing local movements, based on solidarity and a new concept of politics, which could favor the universalization of health.

Global Health↗

PubMedDate not supplied

[Value of electromyography in the thoracic outlet syndrome (author's transl)].

Electromyogram studies were conducted in 55 patients with the thoracic outlet syndrome, together with the motor nerve conduction velocity (M.N.C.V) in the median cubital nerves, between the Erb's point and axilla and in the upper limb. Distal S.N.C.V. was measured in 19 cases. Proximal nerve compression was confirmed in 29% of cases, with associated carpal canal compression in 22%. The proximal N.C.V. of the median and cubital in the neurological forms, and of the median in the neurovascular forms, were significantly different from normal values in 18 control subjects, but were not so in the vascular forms. Through E.M.G. examination cannot confirm the need for surgery it can supply supportive evidence. No correlation between the clinical course and the proximal N.C.V. was found in the 18 patients seen after treatment (surgery in 13 cases).

Adolescent↗

PubMedDate not supplied

The role of T helper lymphocyte subsets in antiviral immunity.

Explore the source record for details and available documents.

Acquired Immunodeficiency Syndrome↗

PubMedDate not supplied

[Comparison of conductivity with sodium determination in the same sweat sample]

OBJECTIVE: To correlate sweat conductivity by Wescor with quantitative sodium determination in the same sample. METHODS: We analyzed 206 sweat samples, being 31 obtained from patients with cystic fibrosis (CF). Sweat was collected by quantitative pilocarpine iontophoresis in the Macroduct system for 30 minutes. Samples were immediately determined by Sweat-Chek analyzer (Wescor) and sodium quantification with a flame photometer. RESULTS: In non-CF subjects sweat (n=175), mean conductivity and quantitative sodium were 41mmol/L (16-75mmol/l) and 36mEq/ l (12-75mEq/l) respectively. In CF subjects (n=31), mean values of conductivity and sodium were 119mmol/L (84-155mmol/l) and 113mEq/l (80 - 146mEq/l) respectively. None of CF patients showed values lower than 80mmol/L. There was a correlation between sweat conductivity and sodium determination (r= + 0.99; p<0.0001). CONCLUSION: Sweat conductivity by Wescor simplifies the analysis with lower volumes and is well correlated to sodium concentration.

Journal Article↗