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Biomedical subjects

A Ferretti

Publications and source records attributed to A Ferretti.

At least 109 records · Page 6Linked to original sources
PubMed1979

Simultaneous mass spectrometric measurement of prostaglandins E1 (PGE1) and PGE2 with a deuterated internal standard.

The use of (3,3,4,4-2H4)PGE2 as internal standard for the quantitative measurement of PGE1 by gas chromatography-mass spectrometry is reported. A method for the simultaneous analysis of PGE1 and PGE2 is also described. The prostaglandins were analyzed by selected ion monitoring as the methyl ester-trimethylsilyl ether (ME-TMS) derivatives of PGB1 and PGB2, respectively. In all cases, a linear response over the range 1 to 70 ng (2.8 to 198 pmoles) was demonstrated. The use of this procedure is exemplified by its application to the PG analysis of sheep seminal vesicles.

Chromatography, Gas↗
PubMed1978

Jumper's knee.

The authors present a clinical study on twenty six cases of a painful syndrome affecting the distal quadriceps tendon complex, popularly known as "jumper's knee". They review the diagnostic aspects and discuss the treatment. In particular, they suggest a surgical approach that is not limited to the inflammatory or degenerative component of the syndrome, but also aims to correct imbalance of the extensor apparatus.

Adult↗
PubMed1977

Tissue variation in hydrocarbon composition in the rabbit.

This study, which deals with the distribution of hydrocarbons in seven types of rabbit tissues, was done for the purpose of providing information that might help shed light on the biological relevance of the hydrocarbons in mammalian metabolism. Liver, kidneys, brain, spleen, skeletal muscle, perinephric adipose, and a sample of blood serum were collected from a single animal for analysis of their hydrocarbon composition. The analytical methodology consisted of solvent extraction, saponification (adipose), elution chromatography on hydrated alumina, and combined gas chromatography-mass spectrometry. Hydrocarbons were detected in all of the tissues examined at concentrations estimated to range from 0.1 to 0.01% of the total lipid extracted Three quite distinct distribution modes were recognized. The bulk of the identified components consisted of normal, saturated, nonterpenoid hydrocarbons in the C16 to C33 range. Squalene, phytene, phytadiene, and pristane were the only terpenoids detected. Nonterpenoid branched (iso and anteiso) hydrocarbons were identified unequivocally and in significant amounts in the muscle only. The adipose was the only tissue which was relatively rich in monoalkenes, and its overall hydrocarbon composition closely resembled that of the feed. The results of the study are not consistent with metabolic inertness. The observed qualitative and quantitative differences might reflect function and metabolic activities of the individual organs in a way yet to be elucidated.

Adipose Tissue↗
PubMed1975

Characterization of two steroidal ketones and two isoprenoid alcohols in dairy products.

Two steroidal ketones, delta-4-cholesten-3-one and delta-3,-5-cholestadiene-7-one, were isolated and identified for the first time in anhydrous milk fat and in nonfat dry milk. Together with these, two isoprenoid alcohols, phytol and dihydrophytol, were identified in anhydrous milk fat. Their identities were established on the basis of chromatographic and mass spectral data and confirmed by comparison with authentic materials.

Animals↗
PubMed1973

Hydrocarbons and polychlorinated biphenyls from the unsaponifiable fraction of anhydrous milk fat.

Using a combination of gas-liquid chromatography and mass spectrometry, the presence of 39 aliphatic hydrocarbons was firmly established in the unsaponifiable fraction of anhydrous milk fat. The hydrocarbons were the C(14) to C(27) and the C(29) to C(31) straight-chain paraffins, their monoolefin analogs, and the C(25) to C(29) branched alkanes. Phytene (3,7,11,15-tetramethyl-n-hexadec-2-ene), identified for the first time in milk fat, was isolated and identified by high-resolution mass spectrometry and infrared analysis. The total hydrocarbon content amounted to 30 ppm of the milk fat. Polychlorinated biphenyls also were detected in trace amounts in the area of the chromatogram between the C(18) and the C(23) hydrocarbons.

Animals↗
PubMedDate not supplied

Hinged Ilizarov external fixation for correction of antebrachial deformities.

OBJECTIVE: To evaluate hinged circular external fixation for correction of antebrachial deformities in dogs. STUDY DESIGN: Uncontrolled clinical trial. ANIMAL POPULATION: Seven client-owned dogs. METHODS: Six dogs had one radius corrected and one dog had both radii corrected. Preoperative planning included measurement of the craniocaudal and mediolateral angular deformities, rotational deformity, length deficit, origin of deformity, graphical or mathematical determination of the amplitude and direction of the actual limb deformity, and frame assembly. RESULTS: Preoperatively, function and cosmesis were assessed to be fair to poor in all dogs. Deformity correction started 48 to 60 hours postoperatively and ranged from 0.46 mm to 1.36 mm twice daily. Hospitalization time ranged from 4 to 6 days. Corrections were mostly made by the owners, at home. Lengthening and angular correction ranged from 3 to 38 mm and 18 degrees to 48 degrees. Mean residual deformities were 2.7% of radial length and 2.7 degrees. The time duration with the circular external fixators in place ranged from 29 to 71 days. Two additional surgeries were necessary in one dog because of wire breakage. Mean follow-up was 40 months. Long-term function and cosmesis were good to excellent in all dogs. CONCLUSION: Although complications were present in six of seven dogs, the outcome of hinged Ilizarov external fixation was successful in all dogs treated for deformities of the antebrachium. CLINICAL RELEVANCE: Despite complex preoperative planning, the placement of hinged circular external fixators is straightforward, and allows precise correction of complex antebrachial deformities with minimal tissue trauma.

Animals↗
PubMedDate not supplied

Regeneration of the semitendinosus tendon harvested for anterior cruciate ligament reconstruction. Evaluation using ultrasonography.

In a prospective study, 40 consecutive patients who underwent anterior cruciate ligament reconstruction with doubled semitendinosus and gracilis tendon autografts were examined pre- and postoperatively by ultrasound to investigate the anatomy of the donor site before and after the harvest of the tendons. The patients underwent ultrasonography at 2 weeks and 1, 2, 3, 6, 12, 18, and 24 months postoperatively. A total of 298 postoperative sonographic evaluations were performed. The semitendinosus tendon was imaged in the sagittal and axial planes: structure and margins were evaluated with the sagittal views; thickness and width were measured with the axial views. In all cases the following sequence of healing was documented: 2 weeks after surgery the semitendinosus tendon site was occupied by an area of increased thickness and decreased echogenicity, suggesting the presence of traumatic edema of the soft tissue surrounding the tenotomy. At 1 month, an irregular hypoechoic structure appeared in a near-anatomic position; at 2 months after surgery, thickness, width, and cross-sectional area of this structure were significantly greater than preoperatively. The amount of regenerated tissue increased up to that seen in the tissue of the 6-month examinations, which also showed a more uniform echostructure. The scans performed at 1 year showed distinct edges and reduction in thickness and width. At 18 and 24 months the echogenicity of the structure occupying the donor site was very similar to that of the normal semitendinosus tendon. However, this structure was clearly identified about 4 cm proximal to the pes anserinus, revealing a more proximal insertion of the regenerated semitendinosus tendon.

Adult↗
PubMedDate not supplied

Hemathrosis treated by aspiration and casting. How to condemn the knee.

Eighty-eight athletes treated for an acute hemarthrosis of the knee without any demonstrable ligament tear were evaluated. Analysis of the clinical data from these cases, all treated between 1970 and 1975 with aspiration and casting, revealed that the injury was apparently worse than had been thought at the time of first treatment. Surgery was subsequently performed in 32 (37%) because of meniscus tear, and 28 demonstrated anterior cruciate ligament insufficiency. In the 56 who did not have surgical treatment, 38 (43% of the entire group) had significant symptoms and disability causing them to decrease sports participation, and only 18 (20% of the overall group) returned to sports without symptoms or disability. Objective findings at followup suggested that in 70% of the patients injury of the anterior cruciate ligament occurred during the original trauma. A precise diagnosis is needed in every knee ligament sprain with hemarthrosis before one decides upon conservative treatment such as aspiration and casting.

Adolescent↗
PubMedDate not supplied

Jumper's knee.

Jumper's knee (patellar or quadriceps tendon tendonitis) is found in a high number of athletes, especially in volleyball and basketball players. Conservative treatment (rest, stretching, physical therapy and antiinflammatory drugs) is usually successful. The athletes often recover completely and resume their sports activity. The purpose of this study is to present the histologic findings and our surgical repair of 18 knees of patients who underwent surgery after failure of conservative treatment. Histologic findings confirm that the so-called "jumper's knee" is a pathology localized at the bone-tendon junction. In all cases the following abnormalities were found: pseudocystic cavities at the borderline between mineralized fibrocartilage and bone, disappearance of the "blue line," increased thickness of the insertional fibrocartilage with myxomatous and hyaline metaplasia, mineralization, and ossification of the fibrocartilage far from the "blue line." Abnormalities of the patellar tendon were observed only in one patient who received local injection of corticosteroids. Eleven of the 18 surgically treated knees obtained a satisfactory result with complete resumption of sports activity.

Adult↗
PubMedDate not supplied

Meniscal tears and associated anterior cruciate ligament tears in athletes: course of treatment.

Sixty-two athletes whose injured knees had negligible instability were found to have an absent or functionless anterior cruciate ligament (ACL) at the time of medial meniscectomy. After a mean of 52 months of followup, 51 patients were examined; 24 (47%) had returned to full sports activities, whereas 27 (53%) acknowledged incidents of the knee "giving way" or having completely given up sports because of instability. By analyzing the following variables, the jerk test, the type of meniscal lesion, and the type of sports engaged, we formulated a rating system to be used intraoperatively for determining the need for combining meniscectomy with a ligament reconstruction.

Adolescent↗
PubMedDate not supplied

Evaluation of the results of extensor mechanism reconstruction.

One hundred sixteen patients underwent extensor mechanism reconstruction. Ninety-four returned at followup (3 to 11 years after surgery). Twenty-two patients were lost to followup. Subjectively, 74% of the patients had either an excellent or good result and 26% either a fair or poor result. We found retrospectively, that the patients with either fair or poor results had a stable or only mildly unstable patella preoperatively.

Adolescent↗
PubMedDate not supplied

Chondromalacia and chronic anterior instabilities of the knee.

In this study, we surveyed a consecutive series of 500 patients who had undergone an open procedure for chronic anteromedial and/or anterolateral instabilities. Those patients who had an open arthrotomy were separated for analysis. All of the patients were examined before and during surgery. A knee sheet, based on the kind used at the Hughston Orthopaedic Clinic in Columbus, Georgia, was used to record all clinical findings. Chondromalacia of the articular surface of the femur was detected at surgery in 161 patients (32%). A statistical analysis showed that the variables directly influencing degenerative changes of the cartilage are: a previous surgery that did not sufficiently restore joint kinematics (chi square = 20.238, P less than 0.001) and a time lapse of more than 30 months between first trauma and surgery (chi square = 21.736; P = 0.001). A higher score on dynamic (jerk or pivot shift) and static (internal and external anterior drawer) tests, indicating instability, or a meniscal tear alone do not statistically correlate with chondromalacia, but together they influence degenerative changes of the cartilage.

Adult↗
PubMedDate not supplied

Knee ligament injuries in volleyball players.

The authors report a series of 52 cases of serious knee ligament injuries in volleyball players. The most frequent mechanism of injury was landing from a jump in the attack zone. Women were more affected than men. Injuries were more frequent during games than training. Volleyball must then be considered among high-risk sports according to the frequency and gravity of our surgical findings. Results are similar to those obtained in athletes in other sports who underwent the same surgical procedure.

Adolescent↗