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Biomedical subjects

A Ferretti

Publications and source records attributed to A Ferretti.

At least 91 records · Page 5Linked to original sources
PubMed1986

Chronic anterior laxity of the knee: classification and coding of the results of surgical treatment.

Between April 1976 and December 1983 we treated 578 cases of anterior capsuloligamentous laxity of the knee surgically (148 acute and 430 chronic). The chronic cases were treated by a variety of techniques; peripheral plastic repair (191 cases), reconstruction of the anterior cruciate ligament with semitendinosus (127 cases), and with semintendinosus and gracilis (112 cases). To achieve uniformity of classification in such a large number of cases it was necessary to simplify and consolidate the data relative to history, objective examination, operative findings and criteria of evaluation of the results. A personal computer was used to provide rapid processing.

Chronic Disease↗
PubMed1985

The natural history of jumper's knee. Patellar or quadriceps tendonitis.

We have reviewed the clinical records and the results of conservative and surgical treatment of 125 out of 172 cases of Jumper's Knee followed for at least 2 years since the onset of symptoms. All were athletes involved in various sporting activities. At follow up 15 patients had given up sport and the lesion had healed spontaneously. We used the classification of Blazina et al as modified by Roels et al. Twenty-four patients were in stage 1, 42 in stage 2, 43 in stage 3 and one in stage 4. Conservative treatment was used primarily on all patients with one exception where the tendon had ruptured. The results were very good in 42, good in 32, and poor in 43, but in 36 patients a long period of rest and reduction in sporting activity was necessary before a significant improvement was obtained. Operation was carried out on 16 patients (19 knees), 18 were in stage 3 and one in stage 4. Various surgical techniques were used. The results were very good in 7 knees, good in 5 and poor in 7. Operation is indicated after the failure of conservative measures and in patients who do not want to reduce their sporting level. The aim should be to induce healing at the bone tendon junction. We emphasize the importance of the prevention of jumper's knee in athletes.

Athletic Injuries↗
PubMed1985

Moderate changes in linoleate intake do not influence the systemic production of E prostaglandins.

A pilot study was undertaken to determine if moderate changes in linoleate (18:2 omega 6) intake would modulate the prostaglandin E turnover concurrently with, or independently of, changes in the plasma prostaglandin (PG) precursor levels. Four adult male volunteers in good health were fed two controlled diets containing 35% of energy from fat, with either 10 (diet L) or 30 g (diet H) linoleate/day, 30 to 50 g saturated fatty acids/day, and the balance mainly monounsaturated fatty acids. All four subjects were consuming sufficient amounts of polyunsaturates before the study. Protein (13-14%) and carbohydrate (51-53%) contribution to total caloric intake was kept constant. The menu cycle was 7 days, and all diets were calculated to provide adequate amounts of nutrients known to be required by man when data were available. Plasma fatty acids were determined by gas-liquid chromatography, and the turnover of E prostaglandins was assessed by measuring the urinary output of the major metabolite of PGE1 + PGE2 (PGE-M). Whereas we found a clear correlation between 18:2 omega 6 intake and 18:2 omega 6 concentrations in the neutral lipid (P = 0.007) and phosphoglyceride (P = 0.012) fractions of plasma, arachidonate (20:4 omega 6) concentrations in those same plasma fractions did not respond significantly to changes in linoleate intake. Moreover, we could not detect an influence of moderate changes in dietary levels of 18:2 omega 6 on the systemic production of PGE as measured by the daily urinary output of PGE-M.

Adult↗
PubMed1985

A fish oil diet rich in eicosapentaenoic acid reduces cyclooxygenase metabolites, and suppresses lupus in MRL-lpr mice.

Dietary supplementation of fish oil as the exclusive source of lipid suppresses autoimmune lupus in MRL-lpr mice. This marine oil diet decreases the lymphoid hyperplasia regulated by the lpr gene, prevents an increase in macrophage surface Ia expression, reduces the formation of circulating retroviral gp70 immune complexes, delays the onset of renal disease, and prolongs survival. We show that a fatty acid component uniquely present in fish oil but not in vegetable oil decreases the quantity of dienoic prostaglandin E, thromboxane B, and prostacyclin normally synthesized by multiple tissues, including kidney, lung, and macrophages, and promotes the synthesis of small amounts of trienoic prostaglandin in autoimmune mice. We suggest that this change in endogenous cyclooxygenase metabolite synthesis directly suppresses immunologic and/or inflammatory mediators of murine lupus.

Animals↗
PubMed1984

Use of a homologous internal standard for the quantification of the major metabolite of prostaglandin F2 alpha in human urine by multiple ion analysis.

A gas chromatographic-mass spectrometric method for quantitative determination of 9 alpha, 11 alpha-dihydroxy-15-oxo-2,3,4,5,20-pentanor-19-carboxyprostanoic acid, the major urinary metabolite of prostaglandin F2 alpha (PGF-M), was developed. The metabolite was analyzed as the dimethyl ester-O-methyloxime-bis-trimethylsilyl ether derivative. The internal standard consisted of a mixture of diethyl ester + monoethyl ester-delta-lactone of PGF-M. Those two species were converted to the 1-methyl-20-ethyl ester derivative during the analytical process. Linear standard curves were developed in the range 0 to 100 ng of injected prostaglandin. The method comprised extraction with Amberlite XAD-2, methylation, chromatography over octadecasilyl-silica, delactonization, remethylation, and chromatography over silicic acid and Lipidex-5000, followed by methoximation, trimethylsilylation, and instrumental analysis. Interassay coefficient of variation, for the analysis of four identical urine specimens, was 7% and intraassay coefficient of variation, when each sample was injected four times, ranged from 3.2 to 6.0%. Specificity, accuracy, and precision of the method were verified by recovery of the metabolite from two different urine pools. The recovery of authentic, underivatized PGF-M added to urine was 99.1 +/- 2.4% (mean +/- SE, N = 6). The plot of recovered versus added metabolite followed the equation y = 0.936 x + 25.8, with r = 0.9918.

Biotransformation↗
PubMed1984

Arthroscopy in the diagnosis and treatment of meniscal lesions of the knee associated with chronic ligamentous instability.

The role of arthroscopy in the diagnosis and treatment of meniscal lesions of the knee associated with chronic ligamentous instability is discussed and illustrated. Clinical examination and meticulous objective tests are usually sufficient to enable the surgeon to arrive at a correct assessment of the ligamentous instability, but in the associated meniscal lesion arthroscopy plays an important role in disclosing the site, extent and type of the lesion. This may be such that it is amenable to repair by subsequent open operation. However, if this is not the case, arthroscopic removal of such parts of the damaged meniscus as may be necessary to relieve symptoms can be carried out without risk of aggravating the instability by interfering with the healthy peripheral parts of the meniscus. The ideal treatment of selected cases is illustrated in an analysis of 35 such patients selected from a total case material of 456 patients including 116 arthroscopies during the same period. They have been classified into two clinical groups in which the treatment is discussed and the results reported.

Adult↗
PubMed1983

Quantitative analysis of 11 alpha-hydroxy-9,15-dioxo-2,3,4,5,20-pentanor-19-carboxyprostanoic acid, the major urinary metabolite of E prostaglandins in man.

A system for the assay of the major metabolite of E prostaglandins (PGE-M) in human urine was developed. The metabolite, as the dimethyl ester-bis(O-methyloxime)-trimethylsilyl ether derivative, was quantified by gas chromatography-mass spectrometry with selected ion monitoring. The internal standard was the diethyl ester analog. A linear response ratio over the range 10 to 300 ng of injected prostaglandin was demonstrated in calibrations with authentic material. The 20-ml specimens of urine were extracted with Amberlite XAD-2, methylated, chromatographed over octadecasilyl-silica, silicic acid, and Lipidex-5000, and then methoximated, trimethylsilylated, and instrumentally analyzed. The interassay coefficient of variation, for analysis of four identical urine specimens, was 11% and the intraassay coefficient of variation ranged from 1 to 5%. Specificity, accuracy, and precision of the method were verified by recovery of PGE-M from four different 80-ml urine pools. Each pool was divided into four equal portions, and different amounts of PGE-M were added to three. The recovery of authentic, underivatized PGE-M added to urine was 103.2 +/- 3.3% (mean +/- SE, N = 12). The plot of recovered versus added PGE-M followed the equation y = 1.003x + 1.83, with R = 0.9974.

Fatty Acids↗
PubMed1983

[Technical variations in abdominal heterotransplants of the heart].

The Authors describe the innovations by them introduced in a series of 15 experiments of abdominal heart heterotopic transplantation done in the Institute of Veterinary Physiology and Biochemistry University of Milano. Particularly they recommend the use of cardioplegia and the prolene as suture's material to reduce the ischemic time. Also they signal the importance of a new position of the transplanted heart with the apex looking at the head of the recipient. They think this position should be the best to preserve the anatomical connections between the great vessels of the guest heart.

Animals↗
PubMed1982

Quantitative measurement of prostaglandins E2 and E2 by selected ion monitoring.

A method for the simultaneous quantitative analysis of prostaglandin E2 (PGE2) and PGE3 is described. The PG were analyzed by selected ion monitoring as the methyl ester-TMS ether derivatives of PGB2 and PGB3, respectively. The internal standard for the quantification of both species was [3,3,4,4-2H4]PGE2. A linear response over the range 0.6--50 ng (1.7--143 pmoles) was demonstrated for PGE3. The chromatographic conditions used (2% SP-2330 column) afforded nearly baseline separation of the prostaglandins. New standard curves for PGE3 must be developed each time the ion source parameters are changed. In a typical calibration run, the instrumental precision, expressed as coefficient of variation, ranged from 1.1 to 7.2% for PGE2 (3 to 100 ng injected) and from 1.6 to 11.1% for PGE3 (1.5--50 ng injected). The method was applied to the PG analysis of rat renomedullary tissues. The recovery of synthetic PGE2 added to medullary homogenates was 100.5 +/- 1.7% (mean +/- SEM, n = 9), and the recovery of PGE3 was 91.3 +/- 1.4% (n = 9).

Animals↗
PubMed1982

[Chronic anteromedial and anterolateral instability of the knee in athletes. Results of treatment with peripheral surgery].

The authors have treated 48 unstable knees by lateral and medial ligament plasties using the Hughston technique. 36 patients were athletes who had to cease their sport because of anteromedial or anterolateral instability. 8 had previously had a menisectomy. Forty-three patients were reviewed with a follow up of 15 to 60 months. 30 patients who had both an active and a passive ligament plasty were able to undertake sporting activities but only 12 at top level. There were 13 failures.

Adolescent↗
PubMed1981

Identification and quantification of prostaglandin E3 in renal medullary tissue of three strains of rats fed fish oil.

Three strains of rats were fed a fish oil diet to verify their ability to incorporate and convert dietary eicosapentaenoic acid (20:5 omega 3) into trienoic prostaglandins. Our results show that such conversion indeed occurs in kidney medullae homogenates. Specifically, the presence of prostaglandin E3 (PGE3) was established by gas chromatographic-mass spectrometric (GC-MS) analysis. That compound was conclusively identified by comparison of fragment ions and their relative intensities with those obtained from authentic PGE3. Further evidence was provided by studying the recovery of exogenously added PGE3. Further evidence was provided by studying the recovery of exogenously added PGE3. The crude ethyl acetate extracts of the medullary homogenates were methylated and cleaned up by liquid-gel chromatography with Lipidex-5000 prior to conversion to PGB3 for GC-MS analysis. The PGE3 was quantified by selected ion monitoring (SIM) with [3,3,4,4-2H4] PGE2 as internal standard. The levels of PGE3 were similar, about 3 ng/mg of wet tissue, in the 3 strains of rats. Identical in vivo conversion of the 2.0:5 omega 3 fatty acid to PGE3 could not be positively established by analysis of pooled urine specimens.

Alprostadil↗
PubMed1981

Production of prostaglandins in homogenates of kidney medullae and cortices of spontaneously hypertensive rats fed menhaden oil.

Menhaden oil (MO), whose polyunsaturated fatty acids consist mainly of (n-3) fatty acids, was fed to spontaneously hypertensive rats to determine the effect of (n-3) fatty acid on the in vitro production of prostaglandins produced from arachidonic acid (20:4[n-6]). Capacity to form PGE2 and PGF2 alpha was impaired in homogenates of kidney medullae and cortices from rats fed the MO diet compared to rats fed the control diet. The lower amounts of diene prostaglandins produced corresponded to the decrease in the amount of 20:4 (n-6) in the tissue. Possibly changes produced in tissue lipids by dietary fatty acids affect prostaglandin production by reducing the availability of substrate in tissue lipids.

Animals↗
PubMed1981

Diffuse skeletal angiomatosis.

Diffuse skeletal angiomatosis is a very rare syndrome. Since it was first observed by Shennan in 1915 only fifty-five cases have been reported in the literature (Rondier et al., 1977). It therefore seems that the description of a new case, accompanied for the first time by ultrastructural findings, may offer a new insight into the pathology of this disease.

Angiomatosis↗
PubMed1979

A simple, rapid selected ion monitoring method for the determination of prostaglandins E2 and F2 alpha in human urine.

A new procedure for the simultaneous measurement of prostaglandins E2 and F2 alpha in human urine is described. Quantification was achieved by gas chromatography mass spectrometry with selected ion monitoring and deuterium labeled internal standards. The levels of measured prostaglandins ranged from 25 pg ml-1 of urine (PGE2) to 2500 pg ml-1 (PGF2 alhpa). The interassay coefficient of variation, determined by replicate analysis of four identical samples, was 1.9% for PGE2 and 0.8% for PGE2 alpha. The procedure takes a fraction of the time needed with published methods and can be conducted with as little as one-tenth of the daily urinary output.

Gas Chromatography-Mass Spectrometry↗