Immunization of humans with tetanus toxoid (TT) results in an inhibition of the reactivity of peripheral lymphocytes to TT as well as to one-way mixed lymphocyte reaction. The strongest inhibition was observed in cells taken 3 or 7 days after immunization. These reactivities reappeared on the 14th day and reached their initial levels by the 21st day after immunization. The role of the various cell types was studied by culturing separately purified T or B lymphocytes taken on day 7 after immunization. B cells alone are not stimulated by TT or allogeneic cells. Purified T cells are stimulated much more than the initial total cell population. When B cells were mixed and cultured along with T cells, a suppressor effect upon stimulation appeared which reduced 3H-thymidine incorporation to the levels of the initial population. Treatment with 5-bromodeoxyuridine followed by exposure to light of cells stimulated with TT abrogated their ability to respond to subsequent stimulation with TT; whereas the responsiveness of treated cells to allogeneic lymphocytes was not significantly affected and vice versa. These results suggest that each stimulator activated a separate population of T cells but that in vivo immunization provoked the development of both a specific and nonspecific suppressor activity. Most probably this suppressor activity was brought about by adherent, surface Ig-bearing (B) cells.

Adult↗

PubMed1976

[Experimental polymyositis induced by the injection of acetylcholine receptors. Study of the lesions in rats and guinea pigs].

The injection of acetylcholine receptors, from the Electrophorus electricus induces in Guinea Pigs and Rats an experimental disease, which is characterized by the appearance of antibodies to sarcolemma and by massive histological alterations of the degenerative type, similar to those observed in clinical polymyositis.

Animals↗

PubMed1976

Mast cell sensitizing antibodies in experimental myositis.

Mast cell sensitizing antibodies, direct mast cell degranulation and positive delayed skin tests were detected in guinea pigs and rats during experimental paralysis provoked by inoculation with acetylcholine receptors from the Electrophorus electricus. The animals also developed fluorescent antibodies to the sarcolemma, and massive myositis with histological changes in muscles similar to those characterizing clinical polymyositis.

Acetylcholine↗

PubMed1975

Antibodies against myoid thymic cells and striated muscle, and monoclonal gammapathy in myasthenia gravis.

The serum of a patient with myasthenia gravis contained a particularly high titer of antistriated muscle antibodies, as well as a monoclonal IgM Kappa immunoglobulin. The antibody activity was only associated with IgG fractions and not with the monoclonal IgM, when these immunoglobulins were separated by chromatography. The association of these two immunological disorders is discussed.

Aged↗

PubMed1975

Titration of antibodies to tetanus toxoid by agglutination of purified tetanus toxoid sensitized latex particles.

The method of sensitized latex particle agglutination which we have devised allows the selection of samples of plasma for the preparation of immunoglobulins. The population of thus selected samples is the same as is selected by the in vivo test, having a titer greater than or equeal to 6 IU/ml. The test gives a clear-cut difference between the positive and negative results and does not give any prozone phenomenon. It can be used extensively, as the batches of sensitized latex particles may be stored for quite a long time.

Adult↗

PubMed1975

Mast cell sensitizing antibodies in myasthenia gravis.

Mast Cells Sensitizing Antibodies (MCSAb) were detected in patients with Myasthenia Gravis (MGr), by the Indirect Mast Cell Degranulation (IMCD). In most of the examined sera a correlation could be established between MCSAb and fluorescent antibodies to muscle and to thymus. The MCSAb were specific for muscle extract. They were resistant to prolonged heating and their reactivity in the IMCD test was not complement depending. Presence of MCSAb in sera of patients with MGr evidences the auto-allergic features of this disease.

Animals↗

PubMed1975