Search PubMed⌕ Search

Biomedical subjects

A Duprez

Publications and source records attributed to A Duprez.

At least 55 records · Page 3Linked to original sources
PubMed1987

[Histo-immunolocalization of human and murine type I and III collagens in human cancers grafted to congenital athymic mice].

Seven human carcinomas and five sarcomas are microsurgically grafted to the nude mouse. We demonstrate with antibodies to human and murin Type I and III collagens that the capsule of the grafted human tumors and the intratumoral perivascular collagen are always of murin origin. Mouse Type I and III collagens take progressively the place of their human counterparts in the extracellular matrix of carcinoma. In contrast the intratumoral collagens of sarcomas are ever of human nature. The murin collagen, probably induced by the tumors, is perhaps one of the factors which prevents in the nude mouse the metastasis of the human grafted tumors.

Animals↗
PubMed1986

[Microsurgical transplantation of bone tumors of uncertain prognosis in athymic nude mice].

Eight cases of human bone or soft tissue tumours were transplanted to nude mice. After such transplants to nude mice which are immunologically deficient, the malignant tumors developed like benign tumours, but maintaining malignant cytological characteristics. The transplants of normal human tissues or of benign tumours decreased in size or remained stable. The technique allowed a change of an original diagnosis of osteosarcoma to a final diagnosis of chondrosarcoma. It made it possible to diagnose a benign osteoblastoma, the diagnosis of which was doubtful before the transplant between osteosarcoma and chondroblastoma. It was possible to diagnose the malignancy of a haemangiopericytoma of muscle. Two aggressive tumours--a non-ossifying fibroma and a giant-cell tumour--were rated as benign after transplantation. This technique also allowed a more precise diagnosis of the grade of one chondrosarcoma and one osteosarcoma. Finally, transplantation also made it possible to test the efficacy of chemotherapy. In a patient so treated, the extreme cell proliferation after transplantation to the nude mouse led to a change in the drugs administered.

Adolescent↗
PubMed1984

Behaviour of thyroid tissue from patients with Graves' disease in nude mice.

Thyroid tissue from five patients with hyperthyroidism due to Graves' disease was transplanted into nu/nu mice (2 to 4 mice per thyroid) in order to assess whether the tissues would remain hyperfunctional. Before surgery, the patients received only propranolol and iodine for ten days. Transplants were removed from the mice after 10, 20, 30 or 57 days, and compared to the initial tissue, as well as toxic nodules from two patients and thyroid tissue from two normal subjects grafted similarly. All transplants survived, as proven by histology and autohistoradiography with 131I uptake, while all signs of hyperfunction and dysimmunity disappeared. Conversely, both transplanted toxic nodules remained hyperfunctional. These results indicate that, in spite of the in situ presence of most factors of auto-immune reactions, thyroid tissue from patients with Graves' disease is not autonomous and depends on the extra-thyroid environment.

Adult↗
PubMed1984

Transplantation of human hyperthyroid tissue to the nude mouse. An experimental model.

This study analyzes the outcome of human normal and hyperfunctioning thyroid tissue transplanted to the nude mouse. Thyroid fragments from 7 patients with Graves' disease were transplanted to nude mice (nu/nu). Before surgery, the patients had been treated with propranolol and iodine; none had received antithyroid therapy. The transplants were removed on the 12th day following transplantation and were studied by light microscopy and autohistoradiography. At this time, all immunologic disorders found on the operative samples had disappeared, and the tissue had lost its hyperfunctioning characteristics. In contrast, transplants from toxic adenoma remained hyperfunctioning, with elevated serum T3 and T4 levels. Similarly, transplants from normal thyroid tissue remained unchanged, and serum T3 and T4 levels remained within the normal range, as if under the influence of the hypothalamic and pituitary regulation of the mice. These findings emphasize the role of the extrathyroid immunologic environment in the regulation of Graves' disease, whereas toxic adenoma remains autonomous.

Adenoma↗
PubMed1982

[Contribution of per- and trans-endoscopic pH measurements to the exploration of the upper digestive tract (author's transl)].

Per- and trans-endoscopic pH measurements in the upper digestive tract have provided new and interesting data. Performed by the authors on a series of 314 patients, they showed that the gastric pH is seldom acid, even in duodenal ulcer, and is always alkaline in gastric ulcer and gastric carcinoma. Gastritis may be divided into two groups, depending on whether the pH is normal or hypoacid (4.5 in the antrum, 2.5 in the fundus). Biopsies demonstrated the presence of intestinal metaplasia and epithelial dysplasia in 57.8% of 83 patients with hypoacid gastritis. The method therefore constitutes a simple and reliable means of determining markers of precancerous lesions.

Esophagoscopy↗
PubMed1980

[Surgical grafting of malignant human melanomas into athymic nu/nu mice : macroscopic and microscopic study].

Tissue from 5 human malignant melanomas was surgically grafted into 39 athymic mice, 20 on lombar muscles covered by their aponevrosis, 13 on the intraperitoneal surface of the abdominal wall, 6 between the dorsal muscles and rib cage. These encapsulated, non-infiltrating, non-destructive tumours were clinically, macroscopically and histologically benign, but cytologically they have a malignant aspect. These were no perceptible visceral, cutaneous or muscular metastases. It would seem that a phenomenon of loosing of invasivness and destruction appeared. However further studies with other tumours and different experimental conditions will be needed to confirm the existence of this phenomenon.

Animals↗
PubMed1980

[Grafts of human skin containing nevi or superficial nevocarcinoma onto athymic nu/nu mice: macroscopic and microscopic studies].

17 thin grafts of corpse skin containing neavi, were grafted onto the back of athymic mice. All the grafts took. The naevi were than deeply incised on 3 occasions. No evidence of abnormal healing, of pigment release, or of malignant transformation, appeared. 10 epidermal grafts of superficial malignant melanoma, taken from 4 different patients, took successfully. In 7 of these, superficial ulcerations appeared but were definitively re-epithelialised from the adjacent Mouse skin. 5 and 7 months later, the melanoma are stable, without local extensions, infiltration or apparent metastase. Despite the absence of an inflammatory reaction, these melanoma are paradoxically quiescent in nude Mice.

Animals↗