Simple test for serological diagnosis of infection with enterotoxigenic Escherichia coli.
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Biomedical subjects
Publications and source records attributed to A Dodin.
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After realization of a new synthetic medium for Whitmore bacillus enrichment and isolation, the authors describe a new specific and immunologic process for Pseudomonas pseudomallei isolation from very polluted samples.
The biological activity of a synthetic fragment of choleric toxin. gamma Chain A subunit, was studied in vivo. This pentadecapeptide (10-24) was synthesized by solid phase method and finally purified by HPLC. Associated with the B subunit, the peptide inhibits the tissue water loss induced by commercial choleric toxin.
The authors describe a new specific and immunologic process for V. cholerae isolation. Specific anti-V. cholerae antibodies (specific IgG anti-fraction Ch 1 + 2) are sticked on magnetic beads. These beads are added to choleric stool. V. cholerae germs stick on the beads. These particles are drawn out of the mixture with the help of a magnet and laid on a Mueller-Hinton agar plate. Incubation is carried out for 18 hours. One drop of toluen is then added on the beads. Wells are sinked in the agar plate and filled with specific serum or antitoxin serum. 12 hours after, the appearance of a precipitation line, facing the specific serum, reveals the presence of toxinogen V. cholerae.
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Two thymus extracts were isolated and partially purified on a Sephadex column. They showed a a molecular weight of 3000 and 12000 respectively. These extracts participate in the protection of the mouse when it has been infected with Yersinia pestis.
Starting from a lysate of Vibrio cholerae, the authors isolated by column chromatography an antigen called Ch1+2 which is characterized by a double line of precipitation by the double gel diffusion technique. (Results of inoculation into animals and humans are given in the following communication).
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