Search PubMed⌕ Search

Biomedical subjects

A Diallo

Publications and source records attributed to A Diallo.

At least 91 records · Page 5Linked to original sources
PubMed1989

Differentiation of rinderpest and peste des petits ruminants viruses using specific cDNA clones.

The morbilliviruses which infect ruminants, rinderpest (RPV) and peste des petits ruminants (PPRV), are difficult to distinguish serologically. They can be distinguished by differential neutralisation tests and by the migration of the major virus structural protein, the nucleocapsid protein, on polyacrylamide gels. Both these methods are time consuming and require the isolation of live virus for identification; they are not suitable for analysis of material directly from post-mortem specimens. We describe a rapid method for differential diagnosis of infections caused by RPV or PPRV, which uses specific cDNA probes, derived from the mRNAs for the nucleocapsid protein of each virus, which can be used to distinguish unequivocally the two virus types rapidly.

Animals↗
PubMed1989

Prevention of tourniquet pain by spinal isobaric bupivacaine with clonidine.

In order to assess the effect of spinal clonidine on tourniquet pain, 30 patients scheduled to undergo orthopaedic surgery under spinal anaesthesia were allocated randomly to two groups. Patients in group I (n = 15) received 0.5% isobaric bupivacaine 15 mg plus isotonic saline 1 ml. Patients in group II (n = 15) received 0.5% bupivacaine 15 mg plus clonidine 1 ml (150 micrograms). Sensory block was evaluated by pinprick and motor block with Bromage's scale. The presence of clonidine significantly prolonged the duration of sensory and motor block. Three patients in group I, but none in group II, experienced tourniquet pain. Hypotension and bradycardia were not worsened by spinal clonidine. The use of clonidine may be a useful technique to augment bupivacaine spinal block.

Adult↗
PubMed1989

Characterization of a new temperature-sensitive and avirulent mutant of the rabies virus.

A temperature-sensitive (ts) mutant, tsG1, has been isolated from the CVS (Challenge Virus Standard) strain of rabies virus. The ts mutation affects the glycoprotein (G protein); it consists of an amino acid substitution (leucine to phenylalanine) at position 132. tsG1 exhibits a slightly reduced pathogenicity when administered via the intracerebral route and complete avirulence after intramuscular inoculation, associated with a very high protective power for adult mice. The ts mutation does not seem to block the transport of the G protein to the plasma membrane at the non-permissive temperature (39.6 degrees C). It abolishes the c.p.e. of the virus in cell cultures.

Amino Acid Sequence↗
PubMed1989

[Attenuation of a strain of rinderpest virus: potential homologous live vaccine].

Peste des petits ruminants (PPR) is a highly contagious disease of small ruminants frequently associated with severe mortality in these hosts. In countries where it occurs, PPR represents an important constraint to the improved productivity of sheep and goats. Until now the only way to combat this plague has been the use of heterologous rinderpest vaccine; all attempts to develop a homologous vaccine have ended in failure. The present communication describes the attenuation of the Nigerian strain PPRV Nig 75/1 by serial passage in Vero cells. The avirulent virus obtained has the same characteristics as Plowright and Ferris' rinderpest vaccine. The virus is advanced as a potential homologous vaccine against PPR.

Animals↗
PubMed1987

Comparison of proteins induced in cells infected with rinderpest and peste des petits ruminants viruses.

The two morbilliviruses rinderpest virus (RPV) and peste des petits ruminants virus (PPRV) are closely related and cause severe disease in large and small ruminants, respectively. They show distinct epidemiological patterns and are distinguishable by reciprocal cross-neutralization tests. We have analysed the proteins induced by these viruses in infected cells and have shown that they can be distinguishable by a very marked difference in the apparent mol. wt. of the nucleocapsid (N) protein. The N protein of PPRV is almost identical in mobility on polyacrylamide gels to the N proteins of measles virus and canine distemper virus (60K). Several strains of RPV and PPRV from widespread geographical locations were studied and found to show this difference in the N protein.

Antibodies, Monoclonal↗
PubMed1986

[Avirulent mutants of the rabies virus: change in site III of the glycoprotein].

Using antiglycoprotein neutralizing monoclonal antibodies, avirulent mutants of rabies virus have been selected. All these mutants have a change in the site III of the glycoprotein: Arginine 333 is replaced by either glutamine, or isoleucine, or glycine. The possibility of selecting avirulent mutants by using neutralizing monoclonal antibodies may allow to get live vaccines and to study the molecular basis of viral virulence.

Animals↗