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Biomedical subjects

A Denys

Publications and source records attributed to A Denys.

At least 109 records · Page 6Linked to original sources
PubMed1989

[Effect of cefoperazone on the activity of selected parameters of cellular immunity in mice with experimental viral-bacterial infection].

We applied mixed, viral-bacterial infections of mice (with influenza virus to the respiratory tract and Staphylococcus aureus) intereperitoneally. We used Sodium Cefoperazone subcutaneously in the dose of 30 mg/kg body weight 24, 48 and 72 hours after the infection. We evaluated phagocytic activity of the granulocytes isolated from animals and bactericidal activity of these cells expressed as their chemiluminescent activity. We studied it on the 3rd, 6th, 9th, and the 14th day after the infection. Phagocytic activity of cells taken from infected mice and treated with the preparation expressed as phagocytic index was the following: 0.38, 0.19; 0.88; 0.99 respectively. In the comparative studies, concerning the effect of preincubation of cells with antibiotic (at the concentration produced in blood serum by therapeutic doses) we found the increase in chemiluminescent process by 57% on the average. Analyzing the preliminary data form the experimental studies on the influence of Cefoperazone on mixed, viral-bacterial infections in mice we found the positive effect of the antibiotic evaluated in some immunological test. Intracellular killing of bacteria is stimulated. Preincubation of granulocytes with the antibiotic gives higher chemiluminescent activity of cells. However, chemotactic and phagocytic activity of cells are not changed.

Animals↗
PubMed1989

[Studies of the effects of BCG and Corynebacterium parvum vaccines in experimental infection of mice with influenza virus. Foot pad test, splenic indicator and histologic changes in the thymus gland, spleen and lymph nodes].

Evaluation of the influence of BCG and Coparvax on reticulo-endothelial system in mouse was performed. Mice were stimulated i.p. with BCG vaccine and Coparvax vaccine. Spleen index and histological changes in thymus, spleen and lymph nodes were evaluated after 14 days in mice vaccinated with BCG and after 7 days in mice vaccinated with Coparvax. Foot pad test was also performed by giving vaccine into three feet. Tuberculin was injected into mouse foot pad on the day 7th and 14th and a lysate of Coparvax vaccine on the day 7th. Spleen index and foot pad test showed higher values in mice vaccinated with Coparvax than with BCG. Histological changes of thymus, spleen, and lymph nodes showed morphological differences depending on the type of vaccine used. Both preparations were characterized by stimulating effect on reticuloendothelial system, which was much more pronounced after giving Coparvax vaccine.

Animals↗
PubMed1989

The effect of Corynebacterium parvum in influenza infected mice.

This paper is the continuation of earlier studies on the effect of the killed suspension of Corynebacterium parvum in influenza virus infected mice. Our investigation showed the normalized effect of these drugs on disturbed function of cell mediated immunity during experimental influenza infection especially in phagocytic and bactericidal activity of granulocytes. The present experiments concern the explanation of these infection mechanisms. Intraperitoneal injection of Corynebacterium parvum stimulated spleen index. Foot pad test is higher than in comparatively treated BCG group. The pathomorphological analysis of the spleen, thymus and peritoneal lymph nodes points out to the multiplication of multiple lymph nodes sinus cells. Generally, C. parvum possessed protective effect in experimental influenza infection. We tested the following parameters: phagocytic and bactericidal activity of granulocytes, liberation of leukocytes migration inhibition factor (LIF).

Adjuvants, Immunologic↗
PubMed1989

[Effect of Corynebacterium parvum preparation on selected immunologic indicators and clinical results in patients with chronic post-traumatic osteomyelitis].

Nineteen patients with chronic post-traumatic staphylococcal osteitis++ of a mean 5-years-clinical course were given intramuscularly 3.5 mg of Coparvax for two weeks. An increase of IgG concentration in the serum, starting from the 8th week of observation, and a marked increase of the ability of absorbtion and destruction of staphylococci by the neutrophils of the peripheral blood have been found. It has been accompanied by subsidence of clinical symptoms of ostitis in 13 patients and a marked decrease of intensity of the inflammatory process in 4 other patients. No effect has been found in two patients.

Adjuvants, Immunologic↗
PubMed1987

Effect of physical exercise on some parameters of immunity in conditioned sportsmen.

The studies were performed on 20 conditioned cyclists and 19 untrained men. At rest absolute and percent number of neutrophils, eosinophils, and monocytes, neutrophil bactericidal activity, and blood plasma beta-glucuronidase, acid phosphatase, and lactic dehydrogenase (LDH) activities were similar in sportsmen and untrained men, while neutrophil adherence was lowered in sportsmen. Maximal physical exercise induced significant rises in absolute numbers of neutrophils and monocytes in both groups. In sportsmen, adherence of neutrophils and monocytes and neutrophil bactericidal activity significantly decreased under the influence of exercise, while neutrophil phagocytic activity did not change. On the other hand, in untrained men, maximal physical exercise did not induce significant changes in neutrophil and monocyte adherence and bactericidal activity of neutrophils, but their phagocytic activity increased. Blood plasma beta-glucuronidase, acid phosphatase, and LDH activities increased during exercise in both groups. The changes observed tended to normalize during 2-h recovery. The results obtained suggest that intensive physical exercise tends to depress nonspecific immunity, which may render sportsmen more susceptible to infections.

Adolescent↗
PubMed1987

Cell phenomena in experimental viral-bacterial infections in mice. I. The release of leukocyte inhibiting factor (LIF) from mouse spleen leukocytes in the presence of specific antigens.

The aim of the presented experiments was to estimate LIF production in the course of experimental infections with influenza viruses (of different degrees of adaptation), with S. aureus 209P and in the course of simultaneous viral-bacterial infections in mice. Spleen cells incubated with specific influenza antigens obtained according to Nath's method were used for the experiments as well as cells incubated with staphylococcal staphylolysine (Boehring Werke). The interdependence between LIF release and the degree of adaptation of viruses to lung tissue was observed. When influenza viruses APR-8 of high degree of adaptation to animal lung tissue were used, inconsiderable LIF production was observed in the early stage of observation. When the animals were infected with viruses A/Scotland/74 of low degree of adaptation, significant LIF production could be noticed since the 1st day of observation. Mixed, viral-bacterial infections influenced insignificantly LIF release. Its production was mainly conditioned by influenza viruses used for the infections.

Adaptation, Physiological↗
PubMed1987

Cell phenomena in experimental viral-bacterial infections in mice. II. Phagocytic activity of granulocytes during infection with influenza viruses and staphylococci.

In the paper the influence of viral, bacterial and mixed viral-bacterial infections on phagocytic activity of mouse peritoneal granulocytes was estimated. Granulocytes isolated on the 3rd, 6th, 9th and 14th day of infection were incubated for 1 h at 37 degrees C together with S. aureus 209P cultivated in the presence of 14C labelled glucose. The radioactivity of samples was measured in the scintillatory counter. The results were shown as phagocytic index of granulocytes. The obtained data point to the decrease of granulocyte phagocytic activity in mice infected with bacteria in the first period of infection. When mice were infected with non-adapted influenza virus the decrease of granulocyte phagocytic activity was observed in the late stage of infection. The infection with adapted virus as well as mixed infection led to the complete disfunction of granulocyte phagocytic activity.

Adaptation, Physiological↗
PubMed1987

Cell phenomena in experimental viral-bacterial infections in mice. III. Estimation of the phagocytic activity of peritoneal granulocytes after in vitro contact with viral and bacterial antigens.

In the paper, the influence of in vitro contact of granulocytes with the antigen obtained from A/Scotland/74 and APR-8 influenza viruses as well as with staphylococcal anatoxine on phagocytic activity of granulocytes was examined. The results are given as phagocytic indices. In the group of animals infected with non-adapted influenza viruses the increase of phagocytosis was observed only in the first period of infection (on the 3rd and 6th day), whereas phagocytic index of granulocytes obtained from animals infected with adapted virus increased only on the 3rd day. In mixed infections, when granulocytes were contacted in vitro with viral as well as bacterial antigens, the insignificant increase of phagocytic indices was observed in the initial period of infection. On the other days of observation (on the 6th, 9th and 14th day) the obtained results approximated to values of phagocytic indices of granulocytes from the control group of animals.

Adaptation, Physiological↗
PubMed1987

Cell phenomena in experimental viral-bacterial infections in mice. IV. Bactericidal activity of peritoneal granulocytes in experimental viral and viral-bacterial infections.

Bactericidal activity of mouse peritoneal granulocytes was examined on the 3rd, 6th, 9th and 14th day after infection. 18-hour-old S. aureus 209P culture was used for examination. Human serum of AB group was used for opsonization. The mixture of bacteria, leukocytes and serum was placed at 37 degrees C for 2 h. Every 30 minutes the samples were taken to estimate the total number of living bacteria and the number of live intracellular bacteria. In the group of animals infected with bacteria as well as in the animals infected with non-adapted influenza viruses, granulocytes showed increased intracellular killing of test bacteria. The decrease of bactericidal activity of granulocytes was observed when they were taken from mice infected with adapted influenza viruses. In mixed infections, with the use of non-adapted virus and bacteria, the decrease of bactericidal activity of granulocytes was observed on the 6th and 9th day after infection. In the group of animals infected simultaneously with non-adapted virus and bacteria, increased bactericidal activity of granulocytes was observed in the initial period of infection. At the same time, on the 14th day this ability significantly decreased.

Animals↗
PubMed1985

The influence of isoprinosine (ISO) on some cell immunity parameters in mice infected with influenza viruses and in mixed viral-bacterial infections. I. The release of the migration inhibiting factor from spleen leukocytes.

The influence of ISO, the antiviral drug of immunomodulating activity, on the course of experimental influenza infections and mixed, viral-bacterial infections was studied. Spleen leukocytes migration inhibition test, performed in vitro in the presence of specific antigens stimulating influence of the drug administered to the infected animals was observed.

Animals↗
PubMed1985

The influence of isoprinosine (ISO) on some cell immunity parameters in mice infected with influenza viruses and in mixed viral-bacterial infections. II. Phagocytosis and bactericidal activity of granulocytes.

The influence of ISO on some stages of the phagocytosis was examined. Namely, the capacity for absorbing and intracellular killing of bacteria in animals infected with influenza viruses and with mixed, viral-bacterial infections was considered. The stimulative influence of the drug was observed. It was particularly visible with the viruses adapted to mice. The influence of the drug is probably in some way specific.

Animals↗