Biomedical subjects

A Coyas

Publications and source records attributed to A Coyas.

26 records · Page 2Linked to original sources

PubMedDate not supplied

Metachromatic reaction and antihistaminic drugs.

We studied the effect of antihistaminic substance on the dye-polysaccharide interaction, using a model in vitro system consisting of heparin-Na and the basic dye o-toluidine blue. The results are recorded on absorption curves in the visible range under standard and modified experimental conditions. We concluded that both histamine and antihistaminic drugs interact in vitro with mucopolysaccharide substances. The results are discussed on a pharmacological basis.

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PubMedDate not supplied

Metachromasia as the key to aminoglycoside ototoxicity. Preliminary report.

In in-vitro experiments, it was shown that aminoglycosides, in contrast to other antibiotics, inhibited the metachromatic reaction using o-toluidine blue as a basic dye and heparin-Na as a polysulfate-polysaccharide substrate. The inhibitory concentrations were inversely proportional to the free amino groups of aminoglycosides tested. Taking into consideration (a) that the aminoglycosides antagonize the presence of Ca++ and (b) that mechanisms of ototoxicity involve ionic alterations in endolymph, we suggest that: aminoglycoside ototoxicity could be the result of the reaction between aminoglycosides and anionic polyelectrolytes, of the group of polysulfated polysaccharides, in a metachromatic process.

Aminoglycosides↗

PubMedDate not supplied