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Biomedical subjects

A Carolei

Publications and source records attributed to A Carolei.

At least 73 records · Page 4Linked to original sources
PubMed1983

Radioimmunological and immunocytochemical demonstration of Met-enkephalin in planaria.

1. An anti-Met-enkephalin serum has been used for radioimmunological and immunocyto-chemical demonstration of Met-enkephalin in planaria. 2. The serum showed a high degree of sensitivity and specificity. 3. Radioimmunoassay demonstrated in planaria extracts a considerable concentration of Met-enkephalin. 4. Met-enkephalin was localized by immunocytochemical methods in neurons and neuropil.

Animals↗
PubMed1983

Effects of dopaminergic agents on monoamine levels and motor behaviour in planaria.

1. Dopamine, serotonin and, in lesser amounts, norepinephrine were detected in Dugesia gonocephala using electrochemical detection coupled with liquid chromatography (LCED). 2. Treatment with L-dopa induced hyperkinesias, and a rise in dopamine, serotonin and norepinephrine content, whereas reserpine reduced motor activity and the concentrations of all three monoamines. 3. Haloperidol reduced motor activity and dopamine and norepinephrine levels. 4. Apomorphine induced hyperkinesias without altering monoamine levels.

Animals↗
PubMed1983

The cerebrum of Dugesia gonocephala s.1. Platyhelminthes, Turbellaria, Tricladida. Morphological and functional observations.

Investigation of the cerebrum of Dugesia gonocephala s.1. using modern techniques has shown that the neurons of these animals situated at the bottom of the phylogenetic scale are basically the same as those of Vertebrates from the ultrastructural and molecular standpoints (tetanus toxin binding). There is no evidence of any biochemical glio-neuronal relationship, nor do the so-called supporting cells appear similar to the glial cells of the higher Vertebrates from this point of view.

Animals↗
PubMed1981

Photosensitization and the nervous system in the planarian Dugesia gonocephala. A histochemical, ultrastructural and behavioral investigation.

Photosensitization phenomena may be induced in planarias by eosin and hematoporphyrin, and as a result, dopamine agonistic behavior ("screw-like hyperkinesia") is set up in the animal. Histochemical, ultrastructural and pharmacological investigations have shown that this hyperkinesia is of post-synaptic origin in eosin photosensitization, and of pre-synaptic origin in hematoporphyrin photosensitization. The authors suggest an hypothesis to explain the different activity of the two photosensitizers, and discuss the validity of the experiment with regard to human porphyria.

Animals↗
PubMed1980

Dose and sex related effects of aromatic aminoacids decarboxylase inhibitors on serum prolactin in humans.

Single doses of carbidopa and benserazide, two inhibitors of aromatic aminoacids decarboxylase, have been given orally to healthy women and men aged 19 to 32 years. Serum prolactin levels increased in respect to baseline levels when 80, 125 or 250 mg of carbidopa and 10, 20, 30, 50, 80 or 125 mg of benserazide were given. The carbidopa doses of 20 and 50 mg were ineffective. Carbidopa induced the enhancement of serum prolactin at a later time and over a longer time span than benserazide. The effect on serum prolactin is dose dependent for both drugs. The hyperprolactinaemia obtained with 250 mg of carbidopa and with 125 mg of benserazide is significantly larger in women than in age matched men. The observed prolactin increase fits with the hypothesis that a diminution of the inhibitory effect of dopamine at the pituitary and/or at the median eminence levels may occur in connection to the pharmacologically impaired monoamine synthesis. An effect of serotonin cannot, however, be excluded.

Administration, Oral↗
PubMed1979

The pigmentary system of planaria. I. Morphology.

The pigmentary system of the planaria, Dugesia gonocephala s.l. (Platyhelminthes, Turbellaria, Tricladida), has been studied by light and electron microscopy. The system consists of granules contained in chromatophore-like cells embedded in the parenchyma. The cell processes penetrate between the muscle layers and extend to the sub-epidermal basal lamina. The nature of the pigment and the comparative anatomical significance of the chromatophore structure is discussed.

Animals↗
PubMed1979

The pigmentary system of planaria. II. Physiology and functional morphology.

The pigmentary system of the planaria, Dugesia gonocephala s.l. (Platyhelminthes, Turbellaria, Tricladida), consists of granules contained in chromatophore cells distributed in the parenchyma tissue. The administration of MSH release-inhibiting Factor (M.I.F.) leads to an easily observable general decolouration of the animal due to the migration of the pigment granules towards the deeper-lying cell nucleus. In planarians bisected transversely through the pharyngeal region, the decolouration occurs only in the cephalic segment, and the caudal segment remains dark. When, however, the decapitated caudal segment regenerates a head region, a decolouration response occurs when exposed to M.I.F. The significance of these results is discussed, and an hypothesis on the hormonal regulation of the pigmentary system is proposed.

Animals↗
PubMed1979

Effects of the inhibition of aromatic aminoacids decarboxylase on prolactin secretion in humans.

Carbidopa, at the dose of 250 mg. and benserazide at the dose of 125 mg, given orally in a single dose to healthy women aged between 23 - 26 years enhance significantly serum prolactin. The effect is not shared by two other inhibitors of AADC, namely alpha-methyl DOPA (500 mg) and fentiazac (400 mg). The effect of benserazide is suppressed by bromocriptine (2.5 mg) and blunted by 1-DOPA (400 mg) given orally simultaneusly.

Adult↗
PubMed1979

Dose and sex related effects of benserazide on prolactin secretion.

Benserazide, given orally at various doses ranging from 10 to 125 mg. to healthy women aged 21 to 33 years induces a dose related increase of prolactin titres in serum. When 125 mg are administered the percent increase of the hormone is larger in women than in men.

Adult↗
PubMed1979

Lack of counteracting effect of liposomes on benserazide-induced hyperprolactinemia.

Benserazide induces an increase of serum prolactin in man, possibly as the result of an impairment of the dopamine effect on the pituitary and/or on the outer median eminence caused by the inhibition on L-dopa decarboxylase. On the other hand, liposomes obtained from bovine brain cortex phospholipids reduced serum prolactin possibly through an effect of phosphatidylserine on dopamine biosynthesis at the level of tyrosine hydroxylase. Benserazide, given orally (125 mg) to 5 normal subjects, induced an increase of serum prolactin that did not change when 300 mg of phospholipid liposomes were given intravenously 60 min later. An increase of L-dopa synthesis does not seen to be capable to overcome the effects of the decarboxylase inhibition.

Adult↗