Immunodiagnosis of human fascioliasis by counterelectrophoresis.
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Biomedical subjects
Publications and source records attributed to A Capron.
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An antigenic component specific to the bloodstream form of serotype AnTat-1 of Trypanosoma brucei brucei was purified by immunoadsorption technique. Subsequently, a monospecific rabbit antiserum was prepared. Purity and specificity controls of the final product were carried out by immunoelectrophoretic analysis.
The enzyme-linked-immunosorbent-assay (E.L.I.S.A.) using whole and purified hydatid antigens was applied to the diagnosis of human hydatid disease. This sensitive and economical method provides new possibilities for the specific diagnosis of parasitic diseases by automated procedures.
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Specific IgE antibodies from immune rat serum were demonstrated to mediate the immune adherence of normal rat macrophages to Schistosome mansoni schistosomules, which was followed by a cytototoc action. Hence, a new positive role of IgE antibodies in protective immunity is argued.
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The comparative immunoelectrophoretic analysis of the antigenic structures of culture and bloodstream forms with cross absorption tests confirm the existence among Trypanosoma gambiense antigens of: --numerous antigenic components common to culture and bloodstream forms;--several antigenic components specific of either culture forms or bloodstream forms. Among the latter, two components are shown to be specific of the antigenic type and appear to support the immunochemical specificity of each variant.
High serum IgE levels were found in eighty-nine patients with hydatid disease. Specific IgE antibodies to E. granulosus hydatid fluid or to a pure E. granulosus antigen were isolated using immunoabsorbents and quantified by a radioimmunoassay. The procedure made it possible to quantify, for the first time, the concentration of IgE antibodies in units per millilitre. Significant levels of IgE antibodies were demonstrated in twenty-three out of thirty-nine patients, by the use of standardized hydatid fluid antigens. The amount of IgE antibodies to E. granulosus was not related to serological findings. However, a highly significant correlation was obtained between levels of total serum IgE and IgE antibodies. A pure antigenic fraction prepared from hydatid fluid and known to be specific for E. granulosus was shown to be the most potent antigen reactive with IgE antibodies. The relationship of IgE antibodies to the viability of the hydatid cyst and to the serum IgE level is discussed.
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