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Biomedical subjects

A Capron

Publications and source records attributed to A Capron.

At least 649 records · Page 36Linked to original sources
PubMed1977

Mother-child relationship in human schistosomiasis mansoni. I. Parasitic antigens and antibodies in milk.

Immunoglobulins, anti-Schistosoma mansoni antibodies, complement components and schistosome antigens were investigated in milk from mothers infected with S. mansoni. No significant differences of immunoglobulins or complement component levels were observed between infected and control mothers. Anti-S. mansoni antibodies were detected in the milk of 8 out of 25 infected mothers. A significant relationship was observed between serum and "4", were demonstrated in milk from infected patients by the double diffusion micromethod. The function of these immunologically active substances transmitted by milk from mother to child is discussed.

Antibodies↗
PubMed1977

Immunological studies in human schistosomiasis. II. Antibodies cytotoxic for Schistosoma mansoni schistosomules.

Complement dependent cytotoxic antibodies for Schistosoma mansoni schistosomules were studied in a Brazilian population. The sera of S. mansoni-infected patients gave a high percentage of cytotoxicity (63.4%) compared with sera from persons not infected (8.7%). The IgG class and the specificity of these cytotoxic antibodies are defined. The index of lethality appeared to be independent of the titers of other specific anti-S. mansoni antibodies. A statistical correlation was found between the index of lethality and both the severity of the disease and delayed hypersensitivity to S. mansoni antigen. The significance of cytotoxic antibodies in human schistosomiasis and their relevance to immunity in man are discussed.

Antibodies↗
PubMed1977

Immunological studies in human schistosomiasis. III. Immunoglobulin levels, antibodies, and delayed hypersensitivity.

Levels of IgG, IgE, IgM, and IgA were determined, specific antibodies were detected by the fluorescent antibody test, hemagglutination test, complement fixation test and immunoelectrophoresis, and intradermal tests for delayed hypersensitivity to Schistosoma mansoni antigens were performed in Brazilian patients with schistosomiasis mansoni. The results were compared according to the clinical forms of the disease. IgG levels and antibody titers increased progressively in the subclinical, hepatomegalic, and hepatosplenic forms and there was a statistical relationship between IgG levels and the intensity of responses to the four serological tests; Delayed hypersensitivity (DHS) was found more frequently in hepatosplenic patients and more particularly in those with splenomegaly. DHS also correlated with age, but not with sex or with skin color. The strongest DHS reactions were observed in patients 20 to 34 years old, and in those having the highest fecal egg output. IgG levels, antibody titers, and DHS responses decreased after splenectomy and portal filtration of the worms. No significant variation was observed between untreated subjects, patients who were splenectomized and a group not subject to reinfection for 4 yearsk0

Antibodies↗
PubMed1977

[Application of CH50 assay to the detection of circulating immune complexes (author's transl)].

Detection and titration of circulating immune complexes appear as an essential tool for the diagnosis and prognostic evaluation of immune complex diseases. Authors propose a simple, rapid, senstive and quantitative method based on the evaluation of complement by soluble immune complexes measured by 50% hemolytic activity. This technique was compared to the I125 C1q binding test and may be recommended for clinical laboratory use.

Antigen-Antibody Complex↗
PubMed1977

Schistosoma haematobium in a Gambian community. IV. Antibody levels and change in egg output.

The indirect fluorescent antibody (FAT) and indirect haemagglutination (IHA) tests were used to study the serological response to Schistosoma haematobium in a Gambian community. The FAT was frequently positive in very young children and also in adults, in whom urine examination did not show ova. This was much less often the case with the IHA although this test sometimes gave negative results in heavily infected children. Examination of paired sera taken at six month intervals apart showed that titres changed in some subjects and that overall there was a slight change in antibody level. Correlation of antibody level with subsequent changes in egg output showed that high titres were associated with a tendency for the egg output to fall. This effect was most obvious over a period of 16 months' follow up and was not explicable solely in terms of a coincidental relationship with age or intensity of infection. It is suggested that serological parameters may have some relation to protective immunity and that the immune response must be considered as a factor in the epidemiology of the infection.

Adolescent↗
PubMed1977

Enzyme immunoassay for the serodiagnosis of parasitic infections.

A review of some applications of the Enzyme-Linked Immunosorbent Assay (ELISA) for the serodiagnosis of parasitic infections is presented. Examples are given of applications with trichinellosis, hydatidosis, schistosomiasis, toxoplasmosis, african trypanosomiasis and amoebiasis. ELISA is believed to be a valuable addition to existing serological tools, especially due to its sensitivity and ease of performance.

Echinococcosis↗
PubMed1977

Circulating immune complexes in schistosomiasis.

Circulating immune complexes (CIC) were investigated by the [125I]Clq binding test, the complement fixation test (CFT) and optical density measurement after redissolving 3% polyethylene glycol precipitates of serum from patients infected by Schistosoma mansoni. A highly significant correlation was obtained among these three techniques. More than 60% of the patients demonstrated significantly higher values than control individuals. The level of CIC was found to be higher in the mild than in the hepatosplenic form of the disease. Parasite antigen, IgG, IgM and IgE were characterized in these CIC. In experimental schistosomiasis in mice, maximum levels of CIC, evaluated by the CFT, were observed between the 40th and the 70th day of infection.

Animals↗
PubMed1977

The use of the radioimmunoprecipitation-PEG assay (RIPEGA) to detect anti-IgG antibodies.

A new procedure, the radioimmunoprecipitation--P.E.G. assay (RIPEGA) is proposed for the detection of anti-IgG antibodies (anti-IgG Ab). This sensitive and reproducible test was performed by incubation of radio-labelled human IgG (125I-IgG) with patient's sera. Separation of free from complexed 125I-IgG was achieved by a 7% P.E.G. precipitation. Preliminary results demonstrated anti-IgG Ab in 84.4% of the cases of sero-positive rheumatoid arthritis (R.A.), 85% of seronegative R.A., 43% of other forms of inflammatory arthritis, 50% of unclassified arthritis and in 10.5% of the control subjects.

Arthritis, Rheumatoid↗
PubMed1977

IgE in experiemental schistosomiasis. I. Serum IgE levels after infection by Schistosoma mansoni in various strains of rats.

Serum IgE levels were measured by radioimmunoassay in four strains of rats, before and after infection by Schistosoma mansoni. Differences were observed in basal IgE levels, depending on the strain considered. Serum IgE rose 10--20 days after infection and the maximum level, between days 30 and 50, ranged from 14 to 60 times the basal level. One of the most characteristic features of IgE production in rat schistosomiasis was the persistence of high IgE levels for a long time after rejection of the worms.

Animals↗
PubMed1976

Detection and characterization of circulating immune complexes in rheumatoid arthritis.

Circulating immune complexes (IC) were detected and quantified in serum of patients with rheumatoid arthritis (RA) by three methods: radiolabeled Clq binding test, anticomplementary activity and optical density of 3% PEG precipitated sera. Rheumatoid factor (RF) is characterized in PEG precipitates by the Waaler-Rose reaction. The relationship between IC, complement and RF levels are analyzed.

Antigen-Antibody Complex↗
PubMed1976

Sensitization to Schistosoma mansoni antigen in uninfected children born to infected mothers.

Sensitization to Schistosoma mansoni antigen in uninfected children born to infected or uninfected mothers was studied by intradermal reaction. Immediate skin reaction, Arthus phenomenon, and delayed skin responses were noted. The skin response at 24 hr was positive in 48.1% of the uninfected children born to infected mothers, and in only 7.5% of uninfected (control) children born to uninfected mothers. Areas of skin reactions were also larger in the group born to infected mothers. Both of these differences were statistically significant. There were no significant variations according to age or sex. Only one immediate skin reaction was noted in each group, and Arthus phenomenon was never observed.

Antigens↗