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Biomedical subjects

A Bergqvist

Publications and source records attributed to A Bergqvist.

At least 73 records · Page 4Linked to original sources
PubMed1987

Endometriosis in the uterosacral ligament giving orthopedic symptoms through compression of the sciatic nerve and surgically treated via an extraperitoneal approach keeping the pelvic organs intact.

Sciatic pain in a young woman was not relieved by orthopedic treatment. The gynecologist found a large hard tumor-like mass in the uterosacral ligament extending to the pelvic wall. Cytologic examination of fine needle aspirate indicated an endometriotic lesion. The large tumor-like mass was extirpated by an extraperitoneal technique and the pain disappeared.

Adult↗
PubMed1986

Characterization and localization of specific oestrogen binding in the human thymus.

Clinical and experimental observations suggest that oestrogens may influence immune reactivity. The oestrogen-binding properties of the human thymus were studied by the use of an aqueous two-phase separation technique. A specific, high-affinity and low-capacity binding was found in 9 out of 10 thymic tissue samples obtained from children and adults of both sexes, their ages ranging from 1 to 42 years. The association constant (+/- SD) for the oestradiol binding in human thymus was 3.7 +/- 1.4 X 10(9) M-1 and was in the same range as the receptor binding in the human uterus. The steroid specificity was found to differ from the classical oestrogen receptor. The concentration of oestrogen-binding sites (+/- SD) was 182 +/- 181 fmol/mg protein and was higher than the receptor concentration of the uterus. According to histochemical analyses the binding is located in the reticulo-epithelial stroma rather than the thymocytes. Data suggest that the human thymus is a target organ for oestrogens which may influence lymphocyte function during therapy.

Adolescent↗
PubMed1986

Late venous function in the leg after deep venous thrombosis occurring in relation to pregnancy.

The frequency of deep venous insufficiency was investigated in 23 patients 3-10 years (mean 7 years) after they had developed an objectively diagnosed deep venous thrombosis (DVT) during pregnancy or the first week after delivery. Investigations included clinical examination, listing of symptoms and plethysmography, venous pressure and Doppler ultrasound. At follow-up there were clinical signs or symptoms of venous insufficiency in 35% of the legs with earlier DVT but in none of the non-thrombosed contralateral legs. Objective evidence of venous insufficiency was present in 15 (65%) of the legs with earlier DVT and in only five (22%) of the non-thrombosed legs. The differences between thrombosed and non-thrombosed legs are statistically significant. There was no correlation between objectively measured venous insufficiency and extent of the DVT. It is concluded that a DVT during pregnancy often leads to venous insufficiency and the risk seems to be higher than after DVT occurring in other groups of patients.

Adolescent↗
PubMed1985

Binding of estrogen and progesterone to human endometrium in the different phases of the menstrual cycle. A histochemical study.

Specific estrogen and progesterone binding in human endometrium was studied histochemically using fluorochrome-labeled steroids (estradiol-17 beta-BSA-FITC and progesterone-BSA-TMRITC), endometrial samples from 36 women being investigated. The binding pattern was similar with both reagents. The relationship between the bindings to glands and to stroma, however, varied with the menstrual phase of the tissue. The specific fluorescence was more intense in the epithelial structures in the proliferative phase. In the secretory phase, the fluorescence from stromal cells was as intense as, or more intense than, that from the glands. The localization of the fluorophores in the glandular epithelial cells also varied by menstrual phase. In the proliferative phase, the fluorescence was most intense in the basal part or the whole cytoplasm of the glandular epithelial cells, while in the secretory phase the fluorescence was most intense in the apical and sometimes also in the basal part of the epithelial cells.

Adult↗
PubMed1985

Histochemical demonstration of estrogen and progesterone binding in endometriotic tissue and in uterine endometrium: A comparative study.

Estrogen and progesterone binding to endometriotic and endometrial tissue was studied histochemically using estradiol and progesterone fluorochrome derivatives (E2-bovine serum albumin-fluorescein isothiocyanate and progesterone-bovine serum albumin-tetramethylrhodamine isothiocyanate). Thirty endometriotic samples from 21 women were studied, together with endometrial specimens obtained simultaneously from 14 of the women. In 77% of the endometriotic samples binding of the estrogen conjugate was indicated by specific fluorescence in more than half of the epithelial cell population, and in 20% in less than half. The corresponding figures for the progesterone conjugate binding were 75 and 18%, respectively. Blocking studies indicated a reasonable degree of ligand specificity. In endometrial tissue the corresponding figures were 64 and 29%, respectively, for binding of the estrogen conjugate and 54 and 38%, respectively, for binding of the progesterone conjugate. In 7 of 13 cases where evaluable samples of both tissues had been obtained, the relative proportion of fluorescent cells, with either reagent, was similar in the two tissue types. Our results suggest that the cytoplasm of epithelial cells in endometriotic tissue and in uterine endometrium contains specific binding sites for both estrogen and progesterone. The binding pattern of the two conjugates in endometriotic tissue was unrelated to the menstrual phase.

Adolescent↗
PubMed1985

Human endometrium transplanted into nude mice. Histologic effects of various steroid hormones.

In a histologic study of human endometrium transplanted into nude mice, eutopic endometrium from each of 8 women was transplanted subcutaneously to 4 nude mice, 1 of which was treated with polyestradiol phosphate, 1 with MPA, 1 with danazol, and 1 of which was left untreated as a control. Grafts were removed after varying intervals of up to 57 days. Residual histologically evaluable endometrial tissue was found in 32 of the 96 grafts (33%). None of the extirpated grafts had the same histologic pattern as the eutopic endometrium. Besides confirming that human endometrium can be transplanted into nude mice, our findings suggest that grafts react histologically in the same way as eutopic human endometrium to hormone treatment. The specific stroma seems to be crucial to a graft's capacity to respond to hormonal stimuli, and its replacement by fibrous tissue was accompanied by glandular epithelial changes very similar to those seen in human endometriotic lesions.

Adult↗
PubMed1985

Human uterine endometrium and endometriotic tissue transplanted into nude mice. Morphologic effects of various steroid hormones.

In a study of the morphologic effects of various steroid hormones on human uterine endometrium and endometriotic tissue, specimens from 6 women were transplanted subcutaneously into 24 nude mice. In each case, specimens collected simultaneously were transplanted to 4 mice, endometrium to one lateral abdominal wall and endometriotic tissue to the other. All mice were given polyestradiol phosphate subcutaneously the first day after operation. After a 2-week interval, of each 4 mice, 1 mouse was given polyestradiol phosphate, 1 medroxyprogesterone acetate, 1 danazol, and 1 no further injections. This treatment continued for 8 weeks, after which the mice were killed and the grafts extirpated. Histologic changes in the grafts varied according to the treatment schedule but were very similar in both types of tissue. Our findings suggest that histologic differences between endometrium and endometriotic tissue seen under natural conditions may, at least partly, be due to variations in environmental factors.

Adult↗
PubMed1984

Human endometrium and endometriotic tissue obtained simultaneously: a comparative histological study.

A comparative study on the histological appearance of endometrium and endometriotic tissue obtained simultaneously has been performed. In all, 47 endometriotic samples from 28 patients were examined. In 15 cases, samples from more than one endometriotic lesion were included. The endometriotic tissue had developed a distinctive menstrual phase pattern in 23 patients (82%). In 16 of these patients (70%), the phase pattern was found to be synchronous (+/- 3 days) in the two tissue types. In seven cases (30%), it varied inconsistently as compared with the endometrial phase. In the remaining five cases (18%), no menstrual phase pattern was found in the endometriotic specimens in spite of a well-defined phase in the endometrium. When biopsy specimens from different endometriotic lesions in the same patient showed a menstrual phase pattern, the phase did not differ significantly among the specimens. Although a menstrual phase pattern could be recognized in the endometriotic tissue samples in most cases, a considerable variability in glandular and epithelial cell morphology was frequently found.

Adult↗
PubMed1984

Histochemical localization of estrogen and progesterone receptors: evaluation of a method.

A histochemical method for the detection of estrogen (ER) and progesterone (PR) receptors in human endometrium, using estrogen and progesterone derivatives linked to fluorochrome-labeled bovine serum albumin (E2-BSA-fluorescein isothiocyanate (FITC) and progesterone-BSA-tetramethylrhodamine isothiocyanate (TMRITC], has been evaluated. The fluorochrome-labeled steroids were bound to the cytoplasm--preferably in glandular epithelial cells but to a lesser extent also to stromal cells. The steroid specificity of the observed binding was studied by preincubating the sections with a series of unlabled steroids and nonsteroidal, hormonally active compounds (estradiol-17 beta, diethylstilbestrol, tamoxifen, 5 alpha-dihydrotestosterone and R 1881 for ER and ORG 2058, R 5020, dexamethasone, cortisol and 5 alpha-dihydrotestosterone for PR). The inhibition studies indicated that E2-BSA-FITC and progesterone-BSA-TMRITC bind to ER and PR in human endometrium with a reasonable degree of specificity. The method was reproducible and various procedural steps were tested, showing satisfactory technical stability. The method is applicable to small tissue samples, and is a valuable complement to quantitative biochemical receptor assays, as it localizes the receptors in tissue slices.

Binding Sites↗
PubMed1984

Histochemical localization of specific estrogen and progesterone binding in human endometrium and endometriotic tissue. A preliminary report.

A histochemical method for the detection of steroid-binding sites in very small tissue specimens was evaluated. Competitive binding experiments proved the method to be sufficiently specific. When applied to endometrium and endometriotic tissue obtained simultaneously from the same patient, a distinct binding was found both in the epithelial structures and in the stroma. The binding pattern in endometriotic glands resembled that in the endometrium. The study seems thus to support the conclusion that endometriotic lesions contain specific binding sites for estrogen and progesterone and that their distribution seems to be similar to that found in the endometrium from the same patient.

Adult↗
PubMed1984

Regression of endometriosis following shorter treatment with, or lower dose of danazol. Comparison of pre- and post-treatment laparoscopic findings in the Scandinavian multi-center study.

One hundred and sixteen patients with laparoscopically confirmed primary or recurrent endometriosis were treated with danazol, either 600 mg daily for 4 months (group A, n = 76) or 600 mg daily for the first 2 months, followed by 400 mg daily for an additional 4 months (group B, n = 40). The only surgery performed before treatment was biopsies, resection of endometriomas greater than or equal to 3 cm and/or adhesiolysis. The extent of endometriosis before and after treatment was established laparoscopically and recorded by means of a modified AFSrecord as mean additive diameter of implants (mean ADI) in millimeters. This provided a uniform and reproducible quantitative registration for each type and location of endometriotic implant. Both treatment schemes resulted in a highly significant (p less than 0.001) reduction of endometriosis, by 79 and 89% in groups A and B, respectively. However, the reduction in mean ADI was significantly greater (p less than 0.025) in group B which had been treated for a longer period. Moreover, the proportion of patients with extensive pre-treatment lesions (mean ADI greater than or equal to 40 mm) was significantly greater in this group. Active residual endometriosis was found in 21 and 17.5% in groups A and B, respectively. These patients had significantly more extensive endometriosis before treatment. The regression of endometriotic implants was independent of type and/or location, i.e. superficial or scarred; peritoneal, ovarian, or tubal. There was no apparent correlation between the quantitative reduction of endometriosis and amenorrhea versus occasional spotting and/or irregular menstruations.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1983

The influence of oral contraceptives on activated factor X inhibitor (XaI)-activity: a prospective study.

A prospective study of activated factor X inhibitor (XaI)-activity has been performed in women randomly allocated to contraceptive pills containing 30 micrograms + 150 micrograms (63 women) or 50 micrograms + 250 micrograms (44 women) ethinyl oestradiol and levonorgestrel, and a reference group of 35 women using other contraceptive methods. The XaI-activity was measured six times during 1 year of observation. There was a significant reduction of XaI-activity after 1 month of oestrogen consumption but there was no significant difference between 30 and 50 micrograms users. The maximal reduction during the observation period was 12% in both groups. There was positive correlation between the pretreatment value and the maximal reduction in the same patient, but the activity did not fall below the normal range (75-125% of normal human plasma).

Adult↗
PubMed1983

Treatment of menorrhagia with intrauterine release of progesterone.

In 12 women with menorrhagia (greater than or equal to 80 ml per menstrual period) a progesterone-releasing device (Progestasert) was inserted. The women were followed by repeated measurements of the blood loss for 12 periods. The menstrual blood loss was significantly reduced after 1 month but the duration of bleeding was somewhat prolonged. Twelve months after insertion the menstrual blood loss amounted to 35% of the blood loss before the insertion. The Progestasert is a valuable treatment for menorrhagia.

Adult↗
PubMed1983

Deep vein thrombosis during pregnancy. A prospective study.

Seventeen women with thrombosis during pregnancy were studied prospectively. The deep venous thrombosis (DVT) was diagnosed objectively with phlebography, plethysmography and thermography. In many cases (13/17) the dominant symptom was diffuse pain in the lower abdomen and/or leg which in several cases caused a delay in the diagnosis. The traditionally typical thrombotic signs were often missing. Proximal thrombi were predominant (13/17) as were left-sided ones (14/17). During pregnancy and lactation the patients were treated with heparin which, after the initial parenteral administration, was given subcutaneously by self-administration. In conclusion, DVT must be suspected and non-invasive diagnostic tests be liberally performed in cases of uncharacteristic lateral and/or declive abdominal pain or leg pain during pregnancy. The DVT must always be objectively verified before treatment. In patients with pregnancy DVT there is no indication for early labor induction and the patients can be delivered by the normal vaginal route. It is recommended that patients with DVT during pregnancy should be treated with heparin subcutaneously which can be self-administered. The post-partum treatment may be continued for 2-3 months with heparin subcutaneously or peroral anticoagulants.

Adolescent↗